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Natl. Acad. Sci. U.S.A."],"published-print":{"date-parts":[[2025,11,11]]},"abstract":"<jats:p>\n                    The cyclic GMP-AMP synthase-Stimulator of Interferon Genes (cGAS\u2013STING) pathway is an important DNA-sensing mechanism that increases T cell trafficking and activation in tumors and reverses the immunosuppressive phenotype of myeloid cells. Therefore, direct STING targeting using synthetic cyclic dinucleotides (CDNs) is an attractive strategy for treating lymphocyte-depleted and myeloid cell\u2013enriched tumors, such as glioblastoma (GBM). However, inadequate bioavailability and poor cellular accumulation limit the clinical development of CDNs, particularly for noninvasive administration strategies. Spherical nucleic acids (SNAs) have emerged as promising modular constructs for creating therapeutic lead compounds for many diseases, including different forms of cancer. Here, we report the development of cGAS-activating SNAs that consist of gold nanoparticle cores functionalized with a shell of densely packed interferon-stimulatory DNA oligonucleotides (ISD\n                    <jats:sup>45<\/jats:sup>\n                    -SNAs). These nanostructures bind to cGAS, the sensor of cytosolic dsDNA upstream of STING, promoting the catalytic production of endogenous CDNs and downstream STING activation more potently than clinically tested CDNs. When administered intranasally or intratumorally to poorly immunogenic syngeneic GBM mouse models, ISD\n                    <jats:sup>45<\/jats:sup>\n                    -SNAs inhibit tumor growth more effectively than CDNs and promote long-term animal subject survival through specific cGAS\u2013STING pathway activation. ISD\n                    <jats:sup>45<\/jats:sup>\n                    -SNAs induce a proinflammatory immune microenvironment enriched with effector T cells and proinflammatory macrophages. When coadministered with immune checkpoint inhibitors (ICI), they abolish GBM tumor development and induce long-term antiglioma immunity. These studies establish ISD\n                    <jats:sup>45<\/jats:sup>\n                    -SNAs as an immune-stimulatory modality for triggering innate and adaptive immune responses and increasing ICI efficacy for GBM treatment.\n                  <\/jats:p>","DOI":"10.1073\/pnas.2409557122","type":"journal-article","created":{"date-parts":[[2025,11,3]],"date-time":"2025-11-03T20:03:55Z","timestamp":1762200235000},"update-policy":"https:\/\/doi.org\/10.1073\/pnas.cm10313","source":"Crossref","is-referenced-by-count":14,"title":["cGAS-agonistic spherical nucleic acids reprogram the glioblastoma immune microenvironment and promote antitumor immunity"],"prefix":"10.1073","volume":"122","author":[{"ORCID":"https:\/\/orcid.org\/0009-0000-0085-555X","authenticated-orcid":false,"given":"Akanksha S.","family":"Mahajan","sequence":"first","affiliation":[{"name":"Department of Neurological Surgery, The Brain Tumor Center, Washington University School of Medicine, Alvin J. Siteman Comprehensive Cancer Center","place":["St. Louis"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Corey","family":"Dussold","sequence":"additional","affiliation":[{"name":"Department of Neurological Surgery, Feinberg School of Medicine, Northwestern University","place":["Chicago"]},{"name":"Malnati Brain Tumor Institute of the Lurie Comprehensive Cancer Center, Feinberg School of Medicine, Northwestern University","place":["Chicago"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Seunghyun","family":"Kim","sequence":"additional","affiliation":[{"name":"Department of Neurological Surgery, The Brain Tumor Center, Washington University School of Medicine, Alvin J. 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Lapointe, A. Bellemare-Pelletier, F. Housseau, J. Thibodeau, P. Hwu, CD40-stimulated B lymphocytes pulsed with tumor antigens are effective antigen-presenting cells that can generate specific T cells. 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