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Natl. Acad. Sci. U.S.A."],"published-print":{"date-parts":[[2024,10]]},"abstract":"<jats:p>\n                    Organ architecture is established during development through intricate cell\u2013cell communication mechanisms, yet the specific signals mediating these communications often remain elusive. Here, we used the anterior pituitary gland that harbors different interdigitated hormone-secreting homotypic cell networks to dissect cell\u2013cell communication mechanisms operating during late development. We show that blocking differentiation of corticotrope cells leads to pituitary hypoplasia with a major effect on somatotrope cells that directly contact corticotropes. Gene knockout of the corticotrope-restricted transcription factor Tpit results in fewer somatotropes, with less secretory granules and a loss of cell polarity, resulting in systemic growth retardation. Single-cell transcriptomic analyses identified\n                    <jats:italic>FGF1<\/jats:italic>\n                    as a corticotrope-specific\n                    <jats:italic>Tpit<\/jats:italic>\n                    dosage-dependent target gene responsible for these phenotypes. Consistently, genetic ablation of\n                    <jats:italic>FGF1<\/jats:italic>\n                    in mice phenocopies pituitary hypoplasia and growth impairment observed in\n                    <jats:italic>Tpit<\/jats:italic>\n                    -deficient mice. These findings reveal FGF1 produced by the corticotrope cell network as an essential paracrine signaling molecule participating in pituitary architecture and size.\n                  <\/jats:p>","DOI":"10.1073\/pnas.2410269121","type":"journal-article","created":{"date-parts":[[2024,9,25]],"date-time":"2024-09-25T13:55:45Z","timestamp":1727272545000},"update-policy":"https:\/\/doi.org\/10.1073\/pnas.cm10313","source":"Crossref","is-referenced-by-count":11,"title":["Paracrine FGF1 signaling directs pituitary architecture and size"],"prefix":"10.1073","volume":"121","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-6049-2356","authenticated-orcid":false,"given":"Konstantin","family":"Khetchoumian","sequence":"first","affiliation":[{"name":"Laboratoire de g\u00e9n\u00e9tique mol\u00e9culaire, Institut de recherches cliniques de Montr\u00e9al, Montr\u00e9al, QC H2W 1R7, Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0002-4891-5819","authenticated-orcid":false,"given":"Kevin","family":"Sochodolsky","sequence":"additional","affiliation":[{"name":"Laboratoire de g\u00e9n\u00e9tique mol\u00e9culaire, Institut de recherches cliniques de Montr\u00e9al, Montr\u00e9al, QC H2W 1R7, Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chrystel","family":"Lafont","sequence":"additional","affiliation":[{"name":"Institute of Functional Genomics, University of Montpellier, CNRS, INSERM, Montpellier F-34094, France"},{"name":"BioCampus Montpellier, University of Montpellier, CNRS, INSERM, Montpellier F-34094, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4224-5770","authenticated-orcid":false,"given":"Arthur","family":"Gouhier","sequence":"additional","affiliation":[{"name":"Laboratoire de g\u00e9n\u00e9tique mol\u00e9culaire, Institut de recherches cliniques de Montr\u00e9al, Montr\u00e9al, QC H2W 1R7, Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Amandine","family":"Bemmo","sequence":"additional","affiliation":[{"name":"Laboratoire de g\u00e9n\u00e9tique mol\u00e9culaire, Institut de recherches cliniques de Montr\u00e9al, Montr\u00e9al, QC H2W 1R7, Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yacine","family":"Kherdjemil","sequence":"additional","affiliation":[{"name":"Disease Modeling and Genome Editing platform, Institut de recherches cliniques de Montr\u00e9al, Montr\u00e9al, QC H2W 1R7, Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marie","family":"Kmita","sequence":"additional","affiliation":[{"name":"Laboratoire de recherche en g\u00e9n\u00e9tique et d\u00e9veloppement, Institut de recherches cliniques de Montr\u00e9al, Montr\u00e9al, QC H2W 1R7, Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Paul","family":"Le Tissier","sequence":"additional","affiliation":[{"name":"Centre for Integrative Physiology, University of Edinburgh, Edinburgh EH8 9XD, United Kingdom"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2324-7589","authenticated-orcid":false,"given":"Patrice","family":"Mollard","sequence":"additional","affiliation":[{"name":"Institute of Functional Genomics, University of Montpellier, CNRS, INSERM, Montpellier F-34094, France"},{"name":"BioCampus Montpellier, University of Montpellier, CNRS, INSERM, Montpellier F-34094, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Helen","family":"Christian","sequence":"additional","affiliation":[{"name":"Department of Physiology, Anatomy and Genetics, Oxford University, Oxford OX1 3QX, United Kingdom"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8226-5691","authenticated-orcid":false,"given":"Jacques","family":"Drouin","sequence":"additional","affiliation":[{"name":"Laboratoire de g\u00e9n\u00e9tique mol\u00e9culaire, Institut de recherches cliniques de Montr\u00e9al, Montr\u00e9al, QC H2W 1R7, Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"341","published-online":{"date-parts":[[2024,9,25]]},"reference":[{"key":"e_1_3_4_1_2","first-page":"1","author":"Drouin J.","year":"2022","unstructured":"J. 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