{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,17]],"date-time":"2026-08-17T22:29:30Z","timestamp":1787005770637,"version":"build-2736575974"},"reference-count":43,"publisher":"National Academy of Sciences","issue":"42","license":[{"start":{"date-parts":[[2024,10,7]],"date-time":"2024-10-07T00:00:00Z","timestamp":1728259200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001809","name":"MOST | National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["62125504"],"award-info":[{"award-number":["62125504"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"MOST | National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["62275232"],"award-info":[{"award-number":["62275232"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"MOST | National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["62035011"],"award-info":[{"award-number":["62035011"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["www.pnas.org"],"crossmark-restriction":true},"short-container-title":["Proc. Natl. Acad. Sci. U.S.A."],"published-print":{"date-parts":[[2024,10,15]]},"abstract":"<jats:p>Cytoskeleton remodeling which generates force and orchestrates signaling and trafficking to govern cell migration remains poorly understood, partly due to a lack of an investigation tool with high system flexibility, spatiotemporal resolution, and computational sensitivity. Herein, we developed a multimodal superresolution imaging system\u2013based architecture-driven quantitative (ADQ) framework in spatiotemporal-angular hyperspace to enable both identification of the optimal imaging mode with well-balanced fidelity and phototoxicity and accurate postcharacterization of microtubule remodeling. In the ADQ framework, a pixel\/voxel-wise metric reflecting heterogeneous intertubule alignment was proposed with improved sensitivity over previous efforts and further incorporated with temporal features to map dynamic microtubule rearrangements. The ADQ framework was verified by assessing microtubule remodeling in drug-induced (de)polymerization, lysosome transport, and migration. Different remodeling patterns from two migration modes were successfully revealed by the ADQ framework, with a front\u2013rear polarization for individual directed migration and a contact site\u2013centered polarization for cell\u2013cell interaction-induced migration in an immune response model. Meanwhile, these migration modes were found to have consistent orientation changes, which exhibited the potential of predicting migration trajectory.<\/jats:p>","DOI":"10.1073\/pnas.2410688121","type":"journal-article","created":{"date-parts":[[2024,10,7]],"date-time":"2024-10-07T15:04:33Z","timestamp":1728313473000},"update-policy":"https:\/\/doi.org\/10.1073\/pnas.cm10313","source":"Crossref","is-referenced-by-count":7,"title":["Architecture-driven quantitative nanoscopy maps cytoskeleton remodeling"],"prefix":"10.1073","volume":"121","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-4575-0983","authenticated-orcid":false,"given":"Wenjie","family":"Liu","sequence":"first","affiliation":[{"name":"State Key Laboratory of Extreme Photonics and Instrumentation, College of Optical Science and Engineering, International Research Center for Advanced Photonics, Zhejiang University, Hangzhou, Zhejiang 310027, China"},{"name":"Zhejiang University (ZJU)-Hangzhou Global Scientific and Technological Innovation Center, Hangzhou, Zhejiang 311200, China"},{"name":"The Kavli Institute for Nanoscience Discovery, University of Oxford, Oxford OX1 3QU, United Kingdom"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1715-6805","authenticated-orcid":false,"given":"Yushi","family":"Yao","sequence":"additional","affiliation":[{"name":"Institute of Immunology, Zhejiang University School of Medicine, Hangzhou, Zhejiang 310058, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jia","family":"Meng","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Extreme Photonics and Instrumentation, College of Optical Science and Engineering, International Research Center for Advanced Photonics, Zhejiang University, Hangzhou, Zhejiang 310027, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shuhao","family":"Qian","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Extreme Photonics and Instrumentation, College of Optical Science and Engineering, International Research Center for Advanced Photonics, Zhejiang University, Hangzhou, Zhejiang 310027, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yubing","family":"Han","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Extreme Photonics and Instrumentation, College of Optical Science and Engineering, International Research Center for Advanced Photonics, Zhejiang University, Hangzhou, Zhejiang 310027, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lingxi","family":"Zhou","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Extreme Photonics and Instrumentation, College of Optical Science and Engineering, International Research Center for Advanced Photonics, Zhejiang University, Hangzhou, Zhejiang 310027, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tao","family":"Wang","sequence":"additional","affiliation":[{"name":"Institute of Immunology, Zhejiang University School of Medicine, Hangzhou, Zhejiang 310058, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Youhua","family":"Chen","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Extreme Photonics and Instrumentation, College of Optical Science and Engineering, International Research Center for Advanced Photonics, Zhejiang University, Hangzhou, Zhejiang 310027, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lingmei","family":"Chen","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Extreme Photonics and Instrumentation, College of Optical Science and Engineering, International Research Center for Advanced Photonics, Zhejiang University, Hangzhou, Zhejiang 310027, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zitong","family":"Ye","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Extreme Photonics and Instrumentation, College of Optical Science and Engineering, International Research Center for Advanced Photonics, Zhejiang University, Hangzhou, Zhejiang 310027, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Liang","family":"Xu","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Extreme Photonics and Instrumentation, College of Optical Science and Engineering, International Research Center for Advanced Photonics, Zhejiang University, Hangzhou, Zhejiang 310027, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1677-3393","authenticated-orcid":false,"given":"Meng","family":"Zhang","sequence":"additional","affiliation":[{"name":"Britton Chance Center for Biomedical Photonics, Wuhan National Laboratory for Optoelectronics-Huazhong University of Science and Technology, Wuhan, Hubei 430074, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jianrong","family":"Qiu","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Extreme Photonics and Instrumentation, College of Optical Science and Engineering, International Research Center for Advanced Photonics, Zhejiang University, Hangzhou, Zhejiang 310027, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tao","family":"Han","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Extreme Photonics and Instrumentation, College of Optical Science and Engineering, International Research Center for Advanced Photonics, Zhejiang University, Hangzhou, Zhejiang 310027, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xu","family":"Liu","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Extreme Photonics and Instrumentation, College of Optical Science and Engineering, International Research Center for Advanced Photonics, Zhejiang University, Hangzhou, Zhejiang 310027, China"},{"name":"Zhejiang University (ZJU)-Hangzhou Global Scientific and Technological Innovation Center, Hangzhou, Zhejiang 311200, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Cuifang","family":"Kuang","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Extreme Photonics and Instrumentation, College of Optical Science and Engineering, International Research Center for Advanced Photonics, Zhejiang University, Hangzhou, Zhejiang 310027, China"},{"name":"Zhejiang University (ZJU)-Hangzhou Global Scientific and Technological Innovation Center, Hangzhou, Zhejiang 311200, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2554-3741","authenticated-orcid":false,"given":"Zhihua","family":"Ding","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Extreme Photonics and Instrumentation, College of Optical Science and Engineering, International Research Center for Advanced Photonics, Zhejiang University, Hangzhou, Zhejiang 310027, China"},{"name":"Zhejiang University (ZJU)-Hangzhou Global Scientific and Technological Innovation Center, Hangzhou, Zhejiang 311200, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhiyi","family":"Liu","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Extreme Photonics and Instrumentation, College of Optical Science and Engineering, International Research Center for Advanced Photonics, Zhejiang University, Hangzhou, Zhejiang 310027, China"},{"name":"Jiaxing Key Laboratory of Photonic Sensing &amp; Intelligent Imaging, Intelligent Optics &amp; Photonics Research Center, Jiaxing Research Institute Zhejiang University, Jiaxing 314000, China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"341","published-online":{"date-parts":[[2024,10,7]]},"reference":[{"key":"e_1_3_5_1_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.1092053"},{"key":"e_1_3_5_2_2","doi-asserted-by":"publisher","DOI":"10.1038\/nrg799"},{"key":"e_1_3_5_3_2","article-title":"Signaling networks that regulate cell migration","volume":"7","author":"Devreotes P.","year":"2015","unstructured":"P. Devreotes, A. R. Horwitz, Signaling networks that regulate cell migration. Csh Perspect. Biol. 7, a005959 (2015).","journal-title":"Csh Perspect. Biol."},{"key":"e_1_3_5_4_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.cub.2003.09.014"},{"key":"e_1_3_5_5_2","doi-asserted-by":"publisher","DOI":"10.1146\/annurev-cellbio-101011-155711"},{"key":"e_1_3_5_6_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0710311105"},{"key":"e_1_3_5_7_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41556-018-0193-1"},{"key":"e_1_3_5_8_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41592-020-0816-x"},{"key":"e_1_3_5_9_2","doi-asserted-by":"publisher","DOI":"10.1364\/OL.445744"},{"key":"e_1_3_5_10_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41592-019-0622-5"},{"key":"e_1_3_5_11_2","doi-asserted-by":"publisher","DOI":"10.1038\/nmeth.1734"},{"key":"e_1_3_5_12_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-018-07244-4"},{"key":"e_1_3_5_13_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41592-019-0611-8"},{"key":"e_1_3_5_14_2","doi-asserted-by":"publisher","DOI":"10.1126\/sciadv.abm2696"},{"key":"e_1_3_5_15_2","doi-asserted-by":"publisher","DOI":"10.1364\/BOE.6.002294"},{"key":"e_1_3_5_16_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.biomaterials.2016.11.041"},{"key":"e_1_3_5_17_2","doi-asserted-by":"publisher","DOI":"10.1038\/nphys1613"},{"key":"e_1_3_5_18_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0960-9822(95)00180-1"},{"key":"e_1_3_5_19_2","doi-asserted-by":"publisher","DOI":"10.1091\/mbc.8.6.973"},{"key":"e_1_3_5_20_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.cels.2016.08.007"},{"key":"e_1_3_5_21_2","doi-asserted-by":"publisher","DOI":"10.1007\/s00281-010-0211-2"},{"key":"e_1_3_5_22_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.coi.2019.02.002"},{"key":"e_1_3_5_23_2","doi-asserted-by":"publisher","DOI":"10.1038\/nrm2713"},{"key":"e_1_3_5_24_2","doi-asserted-by":"publisher","DOI":"10.1242\/jcs.115.17.3527"},{"key":"e_1_3_5_25_2","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.139.2.417"},{"key":"e_1_3_5_26_2","doi-asserted-by":"publisher","DOI":"10.1038\/9018"},{"key":"e_1_3_5_27_2","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.159.3.1063"},{"key":"e_1_3_5_28_2","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.142.1.181"},{"key":"e_1_3_5_29_2","doi-asserted-by":"publisher","DOI":"10.3389\/fimmu.2011.00024"},{"key":"e_1_3_5_30_2","doi-asserted-by":"publisher","DOI":"10.1038\/nature06887"},{"key":"e_1_3_5_31_2","doi-asserted-by":"publisher","DOI":"10.1101\/cshperspect.a006098"},{"key":"e_1_3_5_32_2","doi-asserted-by":"publisher","DOI":"10.15252\/embj.20105114"},{"key":"e_1_3_5_33_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41592-022-01589-x"},{"key":"e_1_3_5_34_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2021.12.045"},{"key":"e_1_3_5_35_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-020-20309-7"},{"key":"e_1_3_5_36_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41592-024-02236-3"},{"key":"e_1_3_5_37_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-021-23449-6"},{"key":"e_1_3_5_38_2","doi-asserted-by":"publisher","DOI":"10.1126\/sciadv.aba4542"},{"key":"e_1_3_5_39_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.aau8302"},{"key":"e_1_3_5_40_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.aaq1392"},{"key":"e_1_3_5_41_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41592-020-00988-2"},{"key":"e_1_3_5_42_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41592-023-01912-0"},{"key":"e_1_3_5_43_2","unstructured":"Z. Liu Order-index. GitHub. https:\/\/github.com\/liuzhiyi07\/Order-index. Deposited 13 August 2023."}],"container-title":["Proceedings of the National Academy of Sciences"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/pnas.org\/doi\/pdf\/10.1073\/pnas.2410688121","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,10,14]],"date-time":"2024-10-14T13:49:20Z","timestamp":1728913760000},"score":1,"resource":{"primary":{"URL":"https:\/\/pnas.org\/doi\/10.1073\/pnas.2410688121"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2024,10,7]]},"references-count":43,"journal-issue":{"issue":"42","published-print":{"date-parts":[[2024,10,15]]}},"alternative-id":["10.1073\/pnas.2410688121"],"URL":"https:\/\/doi.org\/10.1073\/pnas.2410688121","relation":{},"ISSN":["0027-8424","1091-6490"],"issn-type":[{"value":"0027-8424","type":"print"},{"value":"1091-6490","type":"electronic"}],"subject":[],"published":{"date-parts":[[2024,10,7]]},"assertion":[{"value":"2024-05-29","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2024-09-01","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2024-10-07","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e2410688121"}}