{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,31]],"date-time":"2026-10-31T05:20:24Z","timestamp":1793424024544,"version":"4.5.0"},"reference-count":55,"publisher":"National Academy of Sciences","issue":"33","license":[{"start":{"date-parts":[[2024,8,8]],"date-time":"2024-08-08T00:00:00Z","timestamp":1723075200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/100000001","name":"NSF","doi-asserted-by":"publisher","award":["NSF (IOS-1645589 and IOS-2041378)"],"award-info":[{"award-number":["NSF (IOS-1645589 and IOS-2041378)"]}],"id":[{"id":"10.13039\/100000001","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000002","name":"HHS | NIH","doi-asserted-by":"publisher","award":["R35-GM118036-06"],"award-info":[{"award-number":["R35-GM118036-06"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000011","name":"HHMI","doi-asserted-by":"publisher","award":["NA"],"award-info":[{"award-number":["NA"]}],"id":[{"id":"10.13039\/100000011","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000011","name":"HHMI","doi-asserted-by":"publisher","award":["NA"],"award-info":[{"award-number":["NA"]}],"id":[{"id":"10.13039\/100000011","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["www.pnas.org"],"crossmark-restriction":true},"short-container-title":["Proc. Natl. Acad. Sci. U.S.A."],"published-print":{"date-parts":[[2024,8,13]]},"abstract":"<jats:p>\n                    Plants employ distinct mechanisms to respond to environmental changes. Modification of mRNA by\n                    <jats:italic>N<\/jats:italic>\n                    <jats:sup>\u20096<\/jats:sup>\n                    -methyladenosine (m\n                    <jats:sup>6<\/jats:sup>\n                    A), known to affect the fate of mRNA, may be one such mechanism to reprogram mRNA processing and translatability upon stress. However, it is difficult to distinguish a direct role from a pleiotropic effect for this modification due to its prevalence in RNA. Through characterization of the transient knockdown-mutants of m\n                    <jats:sup>6<\/jats:sup>\n                    A writer components and mutants of specific m\n                    <jats:sup>6<\/jats:sup>\n                    A readers, we demonstrate the essential role that m\n                    <jats:sup>6<\/jats:sup>\n                    A plays in basal resistance and pattern-triggered immunity (PTI). A global m\n                    <jats:sup>6<\/jats:sup>\n                    A profiling of mock and PTI-induced\n                    <jats:italic>Arabidopsis<\/jats:italic>\n                    plants as well as formaldehyde fixation and cross-linking immunoprecipitation-sequencing of the m\n                    <jats:sup>6<\/jats:sup>\n                    A reader, EVOLUTIONARILY CONSERVED C-TERMINAL REGION2 (ECT2) showed that while dynamic changes in m\n                    <jats:sup>6<\/jats:sup>\n                    A modification and binding by ECT2 were detected upon PTI induction, most of the m\n                    <jats:sup>6<\/jats:sup>\n                    A sites and their association with ECT2 remained static. Interestingly, RNA degradation assay identified a dual role of m\n                    <jats:sup>6<\/jats:sup>\n                    A in stabilizing the overall transcriptome while facilitating rapid turnover of immune-induced mRNAs during PTI. Moreover, polysome profiling showed that m\n                    <jats:sup>6<\/jats:sup>\n                    A enhances immune-associated translation by binding to the ECT2\/3\/4 readers. We propose that m\n                    <jats:sup>6<\/jats:sup>\n                    A plays a positive role in plant immunity by destabilizing defense mRNAs while enhancing their translation efficiency to create a transient surge in the production of defense proteins.\n                  <\/jats:p>","DOI":"10.1073\/pnas.2411100121","type":"journal-article","created":{"date-parts":[[2024,8,8]],"date-time":"2024-08-08T13:41:12Z","timestamp":1723124472000},"update-policy":"https:\/\/doi.org\/10.1073\/pnas.cm10313","source":"Crossref","is-referenced-by-count":38,"title":["m\n                    <sup>6<\/sup>\n                    A modification plays an integral role in mRNA stability and translation during pattern-triggered immunity"],"prefix":"10.1073","volume":"121","author":[{"given":"Tianyuan","family":"Chen","sequence":"first","affiliation":[{"name":"HHMI, Duke University, Durham, NC 27708"},{"name":"Department of Biology, Duke University, Durham, NC 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Love G. H. Greene J. Motley X. Dong The roles of m(6)A modification during plant pattern-triggered immunity. NCBI BioProject. https:\/\/www.ncbi.nlm.nih.gov\/bioproject\/PRJNA1118749. Deposited 31 May 2024."}],"container-title":["Proceedings of the National Academy of Sciences"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/pnas.org\/doi\/pdf\/10.1073\/pnas.2411100121","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,8,8]],"date-time":"2024-08-08T13:42:46Z","timestamp":1723124566000},"score":1,"resource":{"primary":{"URL":"https:\/\/pnas.org\/doi\/10.1073\/pnas.2411100121"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2024,8,8]]},"references-count":55,"journal-issue":{"issue":"33","published-print":{"date-parts":[[2024,8,13]]}},"alternative-id":["10.1073\/pnas.2411100121"],"URL":"https:\/\/doi.org\/10.1073\/pnas.2411100121","relation":{},"ISSN":["0027-8424","1091-6490"],"issn-type":[{"value":"0027-8424","type":"print"},{"value":"1091-6490","type":"electronic"}],"subject":[],"published":{"date-parts":[[2024,8,8]]},"assertion":[{"value":"2024-06-03","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2024-07-12","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2024-08-08","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e2411100121"}}