{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,11,3]],"date-time":"2026-11-03T23:12:41Z","timestamp":1793747561465,"version":"4.6.1"},"reference-count":46,"publisher":"National Academy of Sciences","issue":"1","license":[{"start":{"date-parts":[[2024,12,30]],"date-time":"2024-12-30T00:00:00Z","timestamp":1735516800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"funder":[{"DOI":"10.13039\/100009633","name":"HHS | NIH | Eunice Kennedy Shriver National Institute of Child Health and Human Development","doi-asserted-by":"publisher","award":["R01HD103064"],"award-info":[{"award-number":["R01HD103064"]}],"id":[{"id":"10.13039\/100009633","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100009633","name":"HHS | NIH | Eunice Kennedy Shriver National Institute of Child Health and Human Development","doi-asserted-by":"publisher","award":["F32HD113220"],"award-info":[{"award-number":["F32HD113220"]}],"id":[{"id":"10.13039\/100009633","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100019180","name":"EC | ERC | HORIZON EUROPE European Research Council","doi-asserted-by":"publisher","award":["101045439"],"award-info":[{"award-number":["101045439"]}],"id":[{"id":"10.13039\/100019180","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100011033","name":"MEC | Agencia Estatal de Investigaci\u00f3n","doi-asserted-by":"publisher","award":["PID2022-143253NB-I00\/ AEI\/10.13039\/501100011033\/ FEDER UE"],"award-info":[{"award-number":["PID2022-143253NB-I00\/ AEI\/10.13039\/501100011033\/ FEDER UE"]}],"id":[{"id":"10.13039\/501100011033","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100004410","name":"European Molecular Biology Organization","doi-asserted-by":"publisher","award":["EMBO ALTF 537-2020"],"award-info":[{"award-number":["EMBO ALTF 537-2020"]}],"id":[{"id":"10.13039\/100004410","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000001","name":"NSF","doi-asserted-by":"publisher","award":["1854642"],"award-info":[{"award-number":["1854642"]}],"id":[{"id":"10.13039\/100000001","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["www.pnas.org"],"crossmark-restriction":true},"short-container-title":["Proc. Natl. Acad. Sci. U.S.A."],"published-print":{"date-parts":[[2025,1,7]]},"abstract":"<jats:p>\n                    In species with genetic sex determination (GSD), the sex identity of the soma determines germ cell fate. For example, in mice, XY germ cells that enter an ovary differentiate as oogonia, whereas XX germ cells that enter a testis initiate differentiation as spermatogonia. However, numerous species lack a GSD system and instead display temperature-dependent sex determination (TSD). In the red-eared slider turtle,\n                    <jats:italic>Trachemys scripta<\/jats:italic>\n                    , a TSD model species with a warm female promoting temperature (FPT) and cool male promoting temperature (MPT) system, temperature directly affects germ cell number. In this study, we examined whether temperature directly affects other aspects of germ cell differentiation\/sex identity. We uncoupled temperature and the sexual fate of the gonad by incubating eggs at MPT and treating with 17\u03b2-estradiol, a scheme that invariably produces ovaries. Through analysis of meiotic spreads, we showed that germ cells in FPT ovaries follow the typical pattern of initiating meiosis and progress through prophase I. However, in E2-induced ovaries that incubated at MPT, germ cells entered prophase I yet fail to exhibit synapsis. These results, combined with our single-cell transcriptome analysis, reveal a direct effect of temperature on germ cell sexual differentiation independent of its effect on the gonadal soma. These results imply that not all events of meiosis are under somatic control, at least not in this TSD species.\n                  <\/jats:p>","DOI":"10.1073\/pnas.2413191121","type":"journal-article","created":{"date-parts":[[2024,12,30]],"date-time":"2024-12-30T15:00:46Z","timestamp":1735570846000},"update-policy":"https:\/\/doi.org\/10.1073\/pnas.cm10313","source":"Crossref","is-referenced-by-count":11,"title":["Gonadal sex and temperature independently influence germ cell differentiation and meiotic progression in\n                    <i>Trachemys scripta<\/i>"],"prefix":"10.1073","volume":"122","author":[{"given":"Talia","family":"Hatkevich","sequence":"first","affiliation":[{"name":"Department of Cell Biology, Duke University Medical Center","place":["Durham"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Boris M.","family":"Tezak","sequence":"additional","affiliation":[{"name":"Department of Cell Biology, Duke University Medical Center","place":["Durham"]},{"name":"Biology Department, Wesleyan University","place":["Middletown"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4120-0140","authenticated-orcid":false,"given":"Rafael D.","family":"Acemel","sequence":"additional","affiliation":[{"name":"Max Delbr\u00fcck Center for Molecular Medicine in the Helmholtz Association, Berlin Institute for Medical Systems Biology, Epigenetics and Sex Development Group","place":["Berlin, Germany"]},{"name":"Centro Andaluz de Biolog\u00eda del Desarrollo, Consejo Superior de Investigaciones Cient\u00edficas, Universidad Pablo de Olavide, Junta de Andaluc\u00eda","place":["Seville, Spain"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Vicky Wai","family":"Yee Chung","sequence":"additional","affiliation":[{"name":"Max Delbr\u00fcck Center for Molecular Medicine in the Helmholtz Association, Berlin Institute for Medical Systems Biology, Epigenetics and Sex Development Group","place":["Berlin, Germany"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3165-036X","authenticated-orcid":false,"given":"Dario G.","family":"Lupi\u00e1\u00f1ez","sequence":"additional","affiliation":[{"name":"Max Delbr\u00fcck Center for Molecular Medicine in the Helmholtz Association, Berlin Institute for Medical Systems Biology, Epigenetics and Sex Development Group","place":["Berlin, Germany"]},{"name":"Centro Andaluz de Biolog\u00eda del Desarrollo, Consejo Superior de Investigaciones Cient\u00edficas, Universidad Pablo de Olavide, Junta de Andaluc\u00eda","place":["Seville, Spain"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6587-0969","authenticated-orcid":false,"given":"Blanche","family":"Capel","sequence":"additional","affiliation":[{"name":"Department of Cell Biology, Duke University Medical Center","place":["Durham"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"341","published-online":{"date-parts":[[2024,12,30]]},"reference":[{"key":"e_1_3_4_1_2","doi-asserted-by":"publisher","DOI":"10.1101\/cshperspect.a016626"},{"key":"e_1_3_4_2_2","doi-asserted-by":"publisher","DOI":"10.1038\/348448a0"},{"key":"e_1_3_4_3_2","doi-asserted-by":"publisher","DOI":"10.1038\/348450a0"},{"key":"e_1_3_4_4_2","doi-asserted-by":"publisher","DOI":"10.1038\/nrg.2017.60"},{"key":"e_1_3_4_5_2","doi-asserted-by":"publisher","DOI":"10.1098\/rstb.1995.0156"},{"key":"e_1_3_4_6_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0012-1606(03)00214-8"},{"key":"e_1_3_4_7_2","doi-asserted-by":"publisher","DOI":"10.1530\/jrf.0.0610461"},{"key":"e_1_3_4_8_2","first-page":"443","article-title":"Kinetics of gametogenesis. I. Comparative histological and autoradiographic studies of oocytes and transitional prospermatogonia during oogenesis and prespermatogenesis","volume":"154","author":"Hilscher B.","year":"1974","unstructured":"B. Hilscher , Kinetics of gametogenesis. I. Comparative histological and autoradiographic studies of oocytes and transitional prospermatogonia during oogenesis and prespermatogenesis. Cell Tissue Res. 154, 443\u2013470 (1974).","journal-title":"Cell Tissue Res."},{"key":"e_1_3_4_9_2","volume-title":"Temperature-Dependent Sex Determination in Vertebrates","author":"Valenzuela N.","year":"2004","unstructured":"N. Valenzuela, V. Lance, Temperature-Dependent Sex Determination in Vertebrates (Smithsonian Books Washington, DC, 2004)."},{"key":"e_1_3_4_10_2","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1097-010X(19980801)281:5<409::AID-JEZ6>3.0.CO;2-S"},{"key":"e_1_3_4_11_2","doi-asserted-by":"publisher","DOI":"10.1139\/z02-111"},{"key":"e_1_3_4_12_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pgen.1004541"},{"key":"e_1_3_4_13_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.1125691"},{"key":"e_1_3_4_14_2","doi-asserted-by":"publisher","DOI":"10.1007\/s00441-023-03814-1"},{"key":"e_1_3_4_15_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.cub.2023.06.008"},{"key":"e_1_3_4_16_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1104501108"},{"key":"e_1_3_4_17_2","doi-asserted-by":"publisher","DOI":"10.1006\/dbio.1994.1093"},{"key":"e_1_3_4_18_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0012-1606(03)00391-9"},{"key":"e_1_3_4_19_2","doi-asserted-by":"publisher","DOI":"10.1101\/gad.9.14.1728"},{"key":"e_1_3_4_20_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0092-8674(00)81312-4"},{"key":"e_1_3_4_21_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0092-8674(00)81876-0"},{"key":"e_1_3_4_22_2","doi-asserted-by":"publisher","DOI":"10.1101\/gad.1612708"},{"key":"e_1_3_4_23_2","doi-asserted-by":"publisher","DOI":"10.1242\/dev.078972"},{"key":"e_1_3_4_24_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0025641"},{"key":"e_1_3_4_25_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1525-142X.2009.00366.x"},{"key":"e_1_3_4_26_2","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.cellbio.19.111301.155141"},{"key":"e_1_3_4_27_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pgen.1007730"},{"key":"e_1_3_4_28_2","doi-asserted-by":"publisher","DOI":"10.1242\/jcs.107.10.2749"},{"key":"e_1_3_4_29_2","doi-asserted-by":"publisher","DOI":"10.3389\/fcell.2022.1009776"},{"key":"e_1_3_4_30_2","doi-asserted-by":"publisher","DOI":"10.3389\/fcell.2023.1147610"},{"key":"e_1_3_4_31_2","doi-asserted-by":"publisher","DOI":"10.1007\/s004120050256"},{"key":"e_1_3_4_32_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.ydbio.2015.05.022"},{"key":"e_1_3_4_33_2","doi-asserted-by":"publisher","DOI":"10.2108\/zsj.13.1"},{"key":"e_1_3_4_34_2","doi-asserted-by":"publisher","DOI":"10.1016\/0016-6480(91)90162-Y"},{"key":"e_1_3_4_35_2","doi-asserted-by":"publisher","DOI":"10.1042\/BC20090021"},{"key":"e_1_3_4_36_2","doi-asserted-by":"publisher","DOI":"10.1242\/dev.194571"},{"key":"e_1_3_4_37_2","doi-asserted-by":"publisher","DOI":"10.1634\/stemcells.2007-0622"},{"key":"e_1_3_4_38_2","doi-asserted-by":"publisher","DOI":"10.1006\/dbio.1999.9600"},{"key":"e_1_3_4_39_2","doi-asserted-by":"publisher","DOI":"10.1095\/biolreprod.103.017541"},{"key":"e_1_3_4_40_2","doi-asserted-by":"publisher","DOI":"10.1242\/dev.107.1.95"},{"key":"e_1_3_4_41_2","doi-asserted-by":"publisher","DOI":"10.1093\/biolre\/ioy214"},{"key":"e_1_3_4_42_2","doi-asserted-by":"publisher","DOI":"10.1002\/jez.1402260218"},{"key":"e_1_3_4_43_2","doi-asserted-by":"publisher","DOI":"10.1242\/dev.001107"},{"key":"e_1_3_4_44_2","doi-asserted-by":"publisher","DOI":"10.1038\/nmeth.2019"},{"key":"e_1_3_4_45_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pgen.1008412"},{"key":"e_1_3_4_46_2","unstructured":"R. D. Acemel Data from \u201cTemperature dependent sex determination in the turtle Trachemys scripta.\u201d Gene Expression Omnibus (GEO). https:\/\/www.ncbi.nlm.nih.gov\/geo\/query\/acc.cgi?acc=GSE271230. Deposited 1 July 2024."}],"container-title":["Proceedings of the National Academy of Sciences"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/pnas.org\/doi\/pdf\/10.1073\/pnas.2413191121","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,12,30]],"date-time":"2024-12-30T15:03:47Z","timestamp":1735571027000},"score":1,"resource":{"primary":{"URL":"https:\/\/pnas.org\/doi\/10.1073\/pnas.2413191121"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2024,12,30]]},"references-count":46,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2025,1,7]]}},"alternative-id":["10.1073\/pnas.2413191121"],"URL":"https:\/\/doi.org\/10.1073\/pnas.2413191121","relation":{},"ISSN":["0027-8424","1091-6490"],"issn-type":[{"value":"0027-8424","type":"print"},{"value":"1091-6490","type":"electronic"}],"subject":[],"published":{"date-parts":[[2024,12,30]]},"assertion":[{"value":"2024-07-05","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2024-10-30","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2024-12-30","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e2413191121"}}