{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,23]],"date-time":"2026-09-23T01:58:07Z","timestamp":1790128687451,"version":"4.0.1"},"reference-count":15,"publisher":"National Academy of Sciences","issue":"26","license":[{"start":{"date-parts":[[2025,6,23]],"date-time":"2025-06-23T00:00:00Z","timestamp":1750636800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100000925","name":"Federal Government | DHAC | National Health and Medical Research Council","doi-asserted-by":"publisher","award":["SRF-B"],"award-info":[{"award-number":["SRF-B"]}],"id":[{"id":"10.13039\/501100000925","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["www.pnas.org"],"crossmark-restriction":true},"short-container-title":["Proc. Natl. Acad. Sci. U.S.A."],"published-print":{"date-parts":[[2025,7]]},"abstract":"<jats:p>\n                    Gastrointestinal (GI) neuroimmune interactions are crucial sensors and regulators of tissue homeostasis. Most enteric neurons reside within the myenteric plexus of the enteric nervous system in the muscular region, forming a structure called the\n                    <jats:italic toggle=\"yes\">muscularis externa<\/jats:italic>\n                    . Despite established interactions between muscularis macrophages and neurons, the presence and function of other immune cell types remains poorly characterized. Here, we mapped the muscularis immune cell landscape, revealing that diverse cell types are present within distinct locations of the GI tract, and they lie in proximity to neuronal cell bodies and their axons. Using a hypothesis-free computational approach, we identify putative ligand\u2013receptor interactions from publicly available single-cell RNA datasets and further validate one of these (App-CD74). This study provides a valuable reference to encourage new avenues of research underpinning enteric neuroimmune interactions as key contributors to GI homeostasis and diseases.\n                  <\/jats:p>","DOI":"10.1073\/pnas.2413692122","type":"journal-article","created":{"date-parts":[[2025,6,23]],"date-time":"2025-06-23T15:00:38Z","timestamp":1750690838000},"update-policy":"https:\/\/doi.org\/10.1073\/pnas.cm10313","source":"Crossref","is-referenced-by-count":9,"title":["Immune cell profiling reveals diverse niches of immune residents of the enteric nervous system and potential neuroimmune interactions"],"prefix":"10.1073","volume":"122","author":[{"given":"Haozhe","family":"Wang","sequence":"first","affiliation":[{"name":"Department of Immunology, School of Translational Medicine, Monash University","place":["Melbourne, Australia"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Aidil","family":"Zaini","sequence":"additional","affiliation":[{"name":"Department of Immunology, School of Translational Medicine, Monash University","place":["Melbourne, Australia"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4686-4210","authenticated-orcid":false,"given":"Bailey","family":"Cardwell","sequence":"additional","affiliation":[{"name":"Department of Immunology, School of Translational Medicine, Monash University","place":["Melbourne, Australia"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0006-6357-2972","authenticated-orcid":false,"given":"Matthew C.","family":"Rowe","sequence":"additional","affiliation":[{"name":"Drug Discovery Biology Theme, Monash Institute of Pharmaceutical Sciences, Monash University","place":["Parkville, Australia"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alana","family":"Butler","sequence":"additional","affiliation":[{"name":"Department of Immunology, School of Translational Medicine, Monash University","place":["Melbourne, Australia"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9020-1847","authenticated-orcid":false,"given":"Connie H. Y.","family":"Wong","sequence":"additional","affiliation":[{"name":"Centre for Inflammatory Diseases, Department of Medicine, School of Clinical Sciences at Monash Health, Monash Medical Centre Monash University","place":["Clayton, Australia"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6168-8422","authenticated-orcid":false,"given":"Daniel P.","family":"Poole","sequence":"additional","affiliation":[{"name":"Drug Discovery Biology Theme, Monash Institute of Pharmaceutical Sciences, Monash University","place":["Parkville, Australia"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Benjamin","family":"Marsland","sequence":"additional","affiliation":[{"name":"Department of Immunology, School of Translational Medicine, Monash University","place":["Melbourne, Australia"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7518-7717","authenticated-orcid":false,"given":"Joel C.","family":"Bornstein","sequence":"additional","affiliation":[{"name":"Department of Anatomy and Physiology, University of Melbourne","place":["Melbourne, Australia"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2922-0210","authenticated-orcid":false,"given":"Nicola L.","family":"Harris","sequence":"additional","affiliation":[{"name":"Department of Immunology, School of Translational Medicine, Monash University","place":["Melbourne, Australia"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"341","published-online":{"date-parts":[[2025,6,23]]},"reference":[{"key":"e_1_3_3_1_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41385-021-00443-1"},{"key":"e_1_3_3_2_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2014.04.050"},{"key":"e_1_3_3_3_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41593-020-00736-x"},{"key":"e_1_3_3_4_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2020.08.003"},{"key":"e_1_3_3_5_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41385-022-00563-2"},{"key":"e_1_3_3_6_2","doi-asserted-by":"publisher","DOI":"10.1093\/ibd\/izae155"},{"key":"e_1_3_3_7_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1114931109"},{"key":"e_1_3_3_8_2","doi-asserted-by":"publisher","DOI":"10.1007\/s00401-010-0774-y"},{"key":"e_1_3_3_9_2","first-page":"559","article-title":"Bacterial growth, flow, and mixing shape human gut microbiota density and composition","volume":"9","author":"Arnoldini M.","year":"2018","unstructured":"M. Arnoldini, J. Cremer, T. Hwa, Bacterial growth, flow, and mixing shape human gut microbiota density and composition. Gut Microbes 9, 559\u2013566 (2018).","journal-title":"Gut Microbes"},{"key":"e_1_3_3_10_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.ade4177"},{"key":"e_1_3_3_11_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-021-21246-9"},{"key":"e_1_3_3_12_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.isci.2023.106277"},{"key":"e_1_3_3_13_2","doi-asserted-by":"publisher","DOI":"10.1186\/1471-2202-10-130"},{"key":"e_1_3_3_14_2","doi-asserted-by":"publisher","DOI":"10.1186\/1750-1326-4-41"},{"key":"e_1_3_3_15_2","doi-asserted-by":"publisher","DOI":"10.1007\/s11481-011-9327-y"}],"container-title":["Proceedings of the National Academy of Sciences"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/pnas.org\/doi\/pdf\/10.1073\/pnas.2413692122","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,6,23]],"date-time":"2025-06-23T15:00:44Z","timestamp":1750690844000},"score":1,"resource":{"primary":{"URL":"https:\/\/pnas.org\/doi\/10.1073\/pnas.2413692122"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,6,23]]},"references-count":15,"journal-issue":{"issue":"26","published-print":{"date-parts":[[2025,7]]}},"alternative-id":["10.1073\/pnas.2413692122"],"URL":"https:\/\/doi.org\/10.1073\/pnas.2413692122","relation":{},"ISSN":["0027-8424","1091-6490"],"issn-type":[{"value":"0027-8424","type":"print"},{"value":"1091-6490","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025,6,23]]},"assertion":[{"value":"2024-07-09","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2025-05-29","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2025-06-23","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e2413692122"}}