{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,28]],"date-time":"2026-10-28T23:34:59Z","timestamp":1793230499978,"version":"4.5.0"},"reference-count":50,"publisher":"National Academy of Sciences","issue":"15","license":[{"start":{"date-parts":[[2025,4,7]],"date-time":"2025-04-07T00:00:00Z","timestamp":1743984000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["www.pnas.org"],"crossmark-restriction":true},"short-container-title":["Proc. Natl. Acad. Sci. U.S.A."],"published-print":{"date-parts":[[2025,4,15]]},"abstract":"<jats:p>\n                    Active regulation of gene expression, orchestrated by complex interactions of activators and repressors at promoters, controls the fate of organisms. In contrast, basal expression at uninduced promoters is considered to be a dynamically inert mode of nonfunctional \u201cpromoter leakiness,\u201d merely a byproduct of transcriptional regulation. Here, we investigate the basal expression mode of the\n                    <jats:italic toggle=\"yes\">mar<\/jats:italic>\n                    operon, the main regulator of intrinsic multiple antibiotic resistance in\n                    <jats:italic toggle=\"yes\">Escherichia coli<\/jats:italic>\n                    , and link its dynamic properties to the noncanonical, yet highly conserved start codon of\n                    <jats:italic toggle=\"yes\">marR<\/jats:italic>\n                    across\n                    <jats:italic toggle=\"yes\">Enterobacteriaceae<\/jats:italic>\n                    . Real-time, single-cell measurements across tens of generations reveal that basal expression consists of rare stochastic gene expression pulses, which maximize variability in wildtype and, surprisingly, transiently accelerate cellular elongation rates. Competition experiments show that basal expression confers fitness advantages to wildtype across several transitions between exponential and stationary growth by shortening lag times. The dynamically rich basal expression of the\n                    <jats:italic toggle=\"yes\">mar<\/jats:italic>\n                    operon has likely been evolutionarily maintained for its role in growth homeostasis of Enterobacteria within the gut environment, thereby allowing other ancillary gene regulatory roles to evolve, e.g., control of costly-to-induce multidrug efflux pumps. Understanding the complex selection forces governing genetic systems involved in intrinsic multidrug resistance is crucial for effective public health measures.\n                  <\/jats:p>","DOI":"10.1073\/pnas.2413709122","type":"journal-article","created":{"date-parts":[[2025,4,7]],"date-time":"2025-04-07T15:01:45Z","timestamp":1744038105000},"update-policy":"https:\/\/doi.org\/10.1073\/pnas.cm10313","source":"Crossref","is-referenced-by-count":6,"title":["Pulsatile basal gene expression as a fitness determinant in bacteria"],"prefix":"10.1073","volume":"122","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-3809-0449","authenticated-orcid":false,"given":"K.","family":"Jain","sequence":"first","affiliation":[{"name":"Institute of Science and Technology Austria","place":["Klosterneuburg, Austria"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9843-3522","authenticated-orcid":false,"given":"R.","family":"Hauschild","sequence":"additional","affiliation":[{"name":"Institute of Science and Technology Austria","place":["Klosterneuburg, Austria"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1006-6639","authenticated-orcid":false,"given":"O. 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