{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,10]],"date-time":"2026-10-10T04:19:17Z","timestamp":1791605957654,"version":"4.3.4"},"reference-count":61,"publisher":"National Academy of Sciences","issue":"3","license":[{"start":{"date-parts":[[2025,1,17]],"date-time":"2025-01-17T00:00:00Z","timestamp":1737072000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"funder":[{"DOI":"10.13039\/100000057","name":"HHS | NIH | National Institute of General Medical Sciences","doi-asserted-by":"publisher","award":["GM124908"],"award-info":[{"award-number":["GM124908"]}],"id":[{"id":"10.13039\/100000057","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000001","name":"NSF","doi-asserted-by":"publisher","award":["CHD-1740656"],"award-info":[{"award-number":["CHD-1740656"]}],"id":[{"id":"10.13039\/100000001","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000015","name":"U.S. Department of Energy","doi-asserted-by":"publisher","award":["DE-AC02-06CH11357"],"award-info":[{"award-number":["DE-AC02-06CH11357"]}],"id":[{"id":"10.13039\/100000015","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000057","name":"HHS | NIH | National Institute of General Medical Sciences","doi-asserted-by":"publisher","award":["GM146107"],"award-info":[{"award-number":["GM146107"]}],"id":[{"id":"10.13039\/100000057","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000057","name":"HHS | NIH | National Institute of General Medical Sciences","doi-asserted-by":"publisher","award":["GM124165"],"award-info":[{"award-number":["GM124165"]}],"id":[{"id":"10.13039\/100000057","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["www.pnas.org"],"crossmark-restriction":true},"short-container-title":["Proc. Natl. Acad. Sci. U.S.A."],"published-print":{"date-parts":[[2025,1,21]]},"abstract":"<jats:p>\n                    Ammonia oxidizing archaea (AOA) are among the most abundant microorganisms on earth and are known to be a major source of nitrous oxide (N\n                    <jats:sub>2<\/jats:sub>\n                    O) emissions, although biochemical origins of this N\n                    <jats:sub>2<\/jats:sub>\n                    O remain unknown. Enzymological details of AOA nitrogen metabolism are broadly unavailable. We report the recombinant expression, purification, and characterization of a multicopper oxidase, Nmar_1354, from the AOA\n                    <jats:italic>Nitrosopumilus maritimus<\/jats:italic>\n                    . We show that Nmar_1354 selectively produces nitroxyl (HNO) by coupling the oxidation of the obligate nitrification intermediate hydroxylamine (NH\n                    <jats:sub>2<\/jats:sub>\n                    OH) to dioxygen (O\n                    <jats:sub>2<\/jats:sub>\n                    ) reduction. This HNO undergoes several downstream reactions, although the major fates are production of N\n                    <jats:sub>2<\/jats:sub>\n                    via reaction with NH\n                    <jats:sub>2<\/jats:sub>\n                    OH and dimerization with itself to yield N\n                    <jats:sub>2<\/jats:sub>\n                    O. These results afford one plausible enzymatic origin for N\n                    <jats:sub>2<\/jats:sub>\n                    O release by AOA. Moreover, these results reveal a physiologically relevant enzymatic reaction for producing HNO, an enigmatic nitrogen oxide speculated to be operative in cellular signaling and in energy transduction.\n                  <\/jats:p>","DOI":"10.1073\/pnas.2416971122","type":"journal-article","created":{"date-parts":[[2025,1,17]],"date-time":"2025-01-17T13:35:51Z","timestamp":1737120951000},"update-policy":"https:\/\/doi.org\/10.1073\/pnas.cm10313","source":"Crossref","is-referenced-by-count":27,"title":["Nitrous oxide production via enzymatic nitroxyl from the nitrifying archaeon\n                    <i>Nitrosopumilus maritimus<\/i>"],"prefix":"10.1073","volume":"122","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-0106-1257","authenticated-orcid":false,"given":"Robert W.","family":"Voland","sequence":"first","affiliation":[{"name":"Department of Chemistry and Chemical Biology, Baker Laboratory, Cornell 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