{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,21]],"date-time":"2026-10-21T16:20:34Z","timestamp":1792599634647,"version":"4.5.0"},"reference-count":41,"publisher":"National Academy of Sciences","issue":"15","license":[{"start":{"date-parts":[[2025,4,10]],"date-time":"2025-04-10T00:00:00Z","timestamp":1744243200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/100000065","name":"HHS | NIH | National Institute of Neurological Disorders and Stroke","doi-asserted-by":"publisher","award":["R37 NS047484"],"award-info":[{"award-number":["R37 NS047484"]}],"id":[{"id":"10.13039\/100000065","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000065","name":"HHS | NIH | National Institute of Neurological Disorders and Stroke","doi-asserted-by":"publisher","award":["RO1 NS105961"],"award-info":[{"award-number":["RO1 NS105961"]}],"id":[{"id":"10.13039\/100000065","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["www.pnas.org"],"crossmark-restriction":true},"short-container-title":["Proc. Natl. Acad. Sci. U.S.A."],"published-print":{"date-parts":[[2025,4,15]]},"abstract":"<jats:p>\n                    Wound healing after spinal cord injury involves highly coordinated interactions among multiple cell types, which are poorly understood. Astrocytes play a central role in creating a border against the non-neural lesion core. To do so, astrocytes undergo dramatic morphological changes by first thickening and elongating their processes and then overlapping them to form a physical barrier. We show here that the expression of a cell-surface receptor, Ryk, is induced in astrocytes after injury in both rodent and human spinal cords. Astrocyte-specific knockout of\n                    <jats:italic>Ryk<\/jats:italic>\n                    dramatically elongated the reactive astrocytes, accelerated the formation of the border, and reduced the size of the scar. Astrocyte-specific knockout of\n                    <jats:italic>Ryk<\/jats:italic>\n                    also accelerated the injury responses of multiple cell types. Single-cell transcriptomics analyses revealed a broad range of changes in cell signaling among astrocytes, microglia, fibroblasts, and endothelial cells after astrocyte-specific\n                    <jats:italic>Ryk<\/jats:italic>\n                    knockout, suggesting that Ryk not only regulates injury responses of astrocytes but may also regulate signals emanating from astrocytes and coordinate the responses of these cell types. The elongation of astrocyte processes is mediated by NrCAM, a cell adhesion molecule induced by astrocyte-specific conditional knockout of\n                    <jats:italic>Ryk<\/jats:italic>\n                    after spinal cord injury. Our findings suggest that Ryk is a promising therapeutic target to accelerate wound healing, promote neuronal survival, and enhance functional recovery.\n                  <\/jats:p>","DOI":"10.1073\/pnas.2417400122","type":"journal-article","created":{"date-parts":[[2025,4,10]],"date-time":"2025-04-10T14:00:06Z","timestamp":1744293606000},"update-policy":"https:\/\/doi.org\/10.1073\/pnas.cm10313","source":"Crossref","is-referenced-by-count":4,"title":["Astrocytic Ryk signaling coordinates scarring and wound healing after spinal cord injury"],"prefix":"10.1073","volume":"122","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-7740-6725","authenticated-orcid":false,"given":"Zhe","family":"Shen","sequence":"first","affiliation":[{"name":"Department of Neurobiology, School of Biological Sciences, University of California","place":["San Diego, La Jolla"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8494-5359","authenticated-orcid":false,"given":"Bo","family":"Feng","sequence":"additional","affiliation":[{"name":"Department of Neurobiology, School of Biological Sciences, University of California","place":["San Diego, La Jolla"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wei Ling","family":"Lim","sequence":"additional","affiliation":[{"name":"Department of Neurobiology, School of Biological Sciences, University of California","place":["San Diego, La Jolla"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Timothy","family":"Woo","sequence":"additional","affiliation":[{"name":"Department of Neurobiology, School of Biological Sciences, University of California","place":["San Diego, La Jolla"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yanlin","family":"Liu","sequence":"additional","affiliation":[{"name":"Department of Neurobiology, School of Biological Sciences, University of California","place":["San Diego, La Jolla"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5670-7169","authenticated-orcid":false,"given":"Silvia","family":"Vicenzi","sequence":"additional","affiliation":[{"name":"Department of Neurobiology, School of Biological Sciences, University of California","place":["San Diego, La Jolla"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jingyi","family":"Wang","sequence":"additional","affiliation":[{"name":"Department of Neurobiology, School of Biological Sciences, University of California","place":["San Diego, La Jolla"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Brian K.","family":"Kwon","sequence":"additional","affiliation":[{"name":"Department of Orthopaedics, International Collaboration on Repair Discoveries, University of British Columbia","place":["Vancouver, Canada"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1092-5547","authenticated-orcid":false,"given":"Yimin","family":"Zou","sequence":"additional","affiliation":[{"name":"Department of Neurobiology, School of Biological Sciences, University of California","place":["San Diego, La Jolla"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"341","published-online":{"date-parts":[[2025,4,10]]},"reference":[{"key":"e_1_3_4_1_2","doi-asserted-by":"publisher","DOI":"10.1177\/0271678X20961852"},{"key":"e_1_3_4_2_2","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.1939-08.2008"},{"key":"e_1_3_4_3_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1206218109"},{"key":"e_1_3_4_4_2","doi-asserted-by":"publisher","DOI":"10.1038\/ncomms7079"},{"key":"e_1_3_4_5_2","doi-asserted-by":"publisher","DOI":"10.1038\/nn.4282"},{"key":"e_1_3_4_6_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41580-022-00562-y"},{"key":"e_1_3_4_7_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.neuron.2012.05.006"},{"key":"e_1_3_4_8_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41586-018-0068-4"},{"key":"e_1_3_4_9_2","doi-asserted-by":"publisher","DOI":"10.1007\/s00441-021-03477-w"},{"key":"e_1_3_4_10_2","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.2121-13.2013"},{"key":"e_1_3_4_11_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.it.2020.07.004"},{"key":"e_1_3_4_12_2","doi-asserted-by":"publisher","DOI":"10.1089\/neu.2012.2613"},{"key":"e_1_3_4_13_2","doi-asserted-by":"publisher","DOI":"10.1038\/nn1520"},{"key":"e_1_3_4_14_2","doi-asserted-by":"publisher","DOI":"10.3389\/fncel.2018.00173"},{"key":"e_1_3_4_15_2","unstructured":"Z. Shen B. Feng T. Woo Y. Zou Single cell sequencing data of mouse spinal cord with dorsal column lesion and astrocyte-specific Ryk conditional knockout. NIH GEO. https:\/\/www.ncbi.nlm.nih.gov\/geo\/query\/acc.cgi?acc=GSE205029. 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