{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,24]],"date-time":"2026-07-24T01:17:45Z","timestamp":1784855865272,"version":"3.55.0"},"reference-count":47,"publisher":"National Academy of Sciences","issue":"48","license":[{"start":{"date-parts":[[2024,11,20]],"date-time":"2024-11-20T00:00:00Z","timestamp":1732060800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"funder":[{"DOI":"10.13039\/100000002","name":"HHS | National Institutes of Health","doi-asserted-by":"publisher","award":["Z01AI000947"],"award-info":[{"award-number":["Z01AI000947"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000002","name":"HHS | National Institutes of Health","doi-asserted-by":"publisher","award":["R01AI134661"],"award-info":[{"award-number":["R01AI134661"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["www.pnas.org"],"crossmark-restriction":true},"short-container-title":["Proc. Natl. Acad. Sci. U.S.A."],"published-print":{"date-parts":[[2024,11,26]]},"abstract":"<jats:p>\n                    The establishment of a productive dengue virus (DENV) infection in the midgut epithelial cells of\n                    <jats:italic>Aedes aegypti<\/jats:italic>\n                    is critical for the viral transmission cycle. The hypothesis that DENV virions interact directly with specific mosquito midgut proteins was explored. We found that DENV serotype 2 (DENV2) pretreated with trypsin interacted with a single 31 kDa protein, identified as AAEL011180 by protein mass spectrometry. This putative receptor is a highly conserved protein and has orthologs in culicine and anopheline mosquitoes. We confirmed that impairing the expression of AAEL011180 in the midgut of\n                    <jats:italic>Ae. aegypti<\/jats:italic>\n                    females abolished the interaction with DENV2, and the virus also bound to immobilized recombinant purified receptor. Furthermore, recombinant DENV2 surface E glycoprotein bound to recombinant AAEL011180 with high affinity (38.2 nM) in binding kinetic analysis using surface plasmon resonance. The gene for this DENV2 E protein receptor (EPrRec) was disrupted, but since the gene is essential in\n                    <jats:italic>Ae. aegypti,<\/jats:italic>\n                    only heterozygote knockout (\u0394EPrRec\n                    <jats:sup>+\/\u2212<\/jats:sup>\n                    ) females could be recovered. Further reducing EPrRec mRNA expression in the midgut of \u0394EPrRec\n                    <jats:sup>+\/\u2212<\/jats:sup>\n                    females by systemic dsRNA injection significantly reduced the prevalence of DENV2 midgut infection. EPrRec also interacts with heat shock protein 70 cognate 3 (Hsc70-3), and silencing Hsc70-3 expression in \u0394EPrRec females also reduced the prevalence of DENV2 midgut infection.\n                  <\/jats:p>","DOI":"10.1073\/pnas.2417750121","type":"journal-article","created":{"date-parts":[[2024,11,20]],"date-time":"2024-11-20T10:30:52Z","timestamp":1732098652000},"update-policy":"https:\/\/doi.org\/10.1073\/pnas.cm10313","source":"Crossref","is-referenced-by-count":6,"title":["Identification of a dengue 2 virus envelope protein receptor in\n                    <i>Aedes aegypti<\/i>\n                    critical for viral midgut infection"],"prefix":"10.1073","volume":"121","author":[{"given":"Asher M.","family":"Kantor","sequence":"first","affiliation":[{"name":"Laboratory of Malaria and Vector Research, National Institutes of Allergy and Infectious Diseases, NIH, Rockville, MD 20852"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Octavio A. 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