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Natl. Acad. Sci. U.S.A."],"published-print":{"date-parts":[[2025,3,18]]},"abstract":"<jats:p>Glaucomatous optic neuropathy, or glaucoma, is the world\u2019s primary cause of irreversible blindness. Glaucoma is comorbid with other neurodegenerative diseases, but how it might impact the environment of the full central nervous system to increase neurodegenerative vulnerability is unknown. Two neurodegenerative events occur early in the optic nerve, the structural link between the retina and brain: loss of anterograde transport in retinal ganglion cell (RGC) axons and early alterations in astrocyte structure and function. Here, we used whole-mount tissue clearing of full mouse brains to image RGC anterograde transport function and astrocyte responses across retinorecipient regions early in a unilateral microbead occlusion model of glaucoma. Using light sheet imaging, we found that RGC projections terminating specifically in the accessory optic tract are the first to lose transport function. Although degeneration was induced in one retina, astrocytes in both brain hemispheres responded to transport loss in a retinotopic pattern that mirrored the degenerating RGCs. A subpopulation of these astrocytes in contact with large descending blood vessels were immunopositive for LCN2, a marker associated with astrocyte reactivity. Together, these data suggest that even early stages of unilateral glaucoma have broad impacts on the health of astrocytes across both hemispheres of the brain, implying a glial mechanism behind neurodegenerative comorbidity in glaucoma.<\/jats:p>","DOI":"10.1073\/pnas.2418249122","type":"journal-article","created":{"date-parts":[[2025,3,10]],"date-time":"2025-03-10T15:06:16Z","timestamp":1741619176000},"update-policy":"https:\/\/doi.org\/10.1073\/pnas.cm10313","source":"Crossref","is-referenced-by-count":12,"title":["Astrocytes in the mouse brain respond bilaterally to unilateral retinal neurodegeneration"],"prefix":"10.1073","volume":"122","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-4337-8356","authenticated-orcid":false,"given":"Melissa L.","family":"Cooper","sequence":"first","affiliation":[{"name":"Institute for Translational Neuroscience, New York University Grossman School of Medicine","place":["New 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for Translational Neuroscience, New York University Grossman School of Medicine","place":["New York"]},{"name":"Department of Neuroscience, New York University Grossman School of Medicine","place":["New York"]},{"name":"Department of Ophthalmology, New York University Langone Health","place":["New York"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6969-3744","authenticated-orcid":false,"given":"Moses V.","family":"Chao","sequence":"additional","affiliation":[{"name":"Institute for Translational Neuroscience, New York University Grossman School of Medicine","place":["New York"]},{"name":"Department of Neuroscience, New York University Grossman School of Medicine","place":["New York"]},{"name":"Department of Psychiatry, New York University Langone Health","place":["New 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