{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,29]],"date-time":"2026-08-29T11:16:21Z","timestamp":1788002181839,"version":"build-2784847793"},"reference-count":78,"publisher":"National Academy of Sciences","issue":"8","license":[{"start":{"date-parts":[[2025,2,21]],"date-time":"2025-02-21T00:00:00Z","timestamp":1740096000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001809","name":"MOST | National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["32371067"],"award-info":[{"award-number":["32371067"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"MOST | National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["32222013"],"award-info":[{"award-number":["32222013"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"MOST | National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["32200829"],"award-info":[{"award-number":["32200829"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["www.pnas.org"],"crossmark-restriction":true},"short-container-title":["Proc. Natl. Acad. Sci. U.S.A."],"published-print":{"date-parts":[[2025,2,25]]},"abstract":"<jats:p>\n                    Newborns, typically asexual, undergo a process of sexual transition to reach sexual maturity, but the regulatory mechanism underlying this transition is not clear. Here, we studied how female sexual behavior is modulated during sexual transition by hormones and neuromodulators in\n                    <jats:italic>Drosophila<\/jats:italic>\n                    . We found that neuropeptide Leucokinin (LK) inhibits female receptivity specifically during a sexual transition period in immature females, but not in younger or mature females. Moreover, the steroid hormone ecdysone, which is mainly synthesized in the female ovary during sexual maturation, acts on LK neurons via the ecdysone receptor to suppress sexual receptivity. We further found that LK suppresses female receptivity through its receptor LKR in central pC1 neurons, a decision center for female sexual behavior. These findings reveal a hormone-to-neuropeptide pathway that specifically inhibits sexual behavior during sexual maturation in female\n                    <jats:italic>Drosophila<\/jats:italic>\n                    , shedding light on how hormones and neuromodulators coordinate sexual development and behaviors.\n                  <\/jats:p>","DOI":"10.1073\/pnas.2418481122","type":"journal-article","created":{"date-parts":[[2025,2,21]],"date-time":"2025-02-21T11:36:47Z","timestamp":1740137807000},"update-policy":"https:\/\/doi.org\/10.1073\/pnas.cm10313","source":"Crossref","is-referenced-by-count":16,"title":["A hormone-to-neuropeptide pathway inhibits sexual receptivity in immature\n                    <i>Drosophila<\/i>\n                    females"],"prefix":"10.1073","volume":"122","author":[{"ORCID":"https:\/\/orcid.org\/0009-0005-1120-8805","authenticated-orcid":false,"given":"Jie","family":"Chen","sequence":"first","affiliation":[{"name":"The Key Laboratory of Developmental Genes and Human Disease, Jiangsu Key Laboratory of Brain Science and Medicine, School of Life Science and Technology, Southeast University","place":["Nanjing, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Peiwen","family":"Zhu","sequence":"additional","affiliation":[{"name":"The Key Laboratory of Developmental Genes and Human Disease, Jiangsu Key Laboratory of Brain Science and Medicine, School of Life Science and Technology, Southeast University","place":["Nanjing, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sihui","family":"Jin","sequence":"additional","affiliation":[{"name":"The Key Laboratory of Developmental Genes and Human Disease, Jiangsu Key Laboratory of Brain Science and Medicine, School of Life Science and Technology, Southeast University","place":["Nanjing, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhaokun","family":"Zhang","sequence":"additional","affiliation":[{"name":"The Key Laboratory of Developmental Genes and Human Disease, Jiangsu Key Laboratory of Brain Science and Medicine, School of Life Science and Technology, Southeast University","place":["Nanjing, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0009-1949-0648","authenticated-orcid":false,"given":"Simei","family":"Jiang","sequence":"additional","affiliation":[{"name":"The Key Laboratory of Developmental Genes and Human Disease, Jiangsu Key Laboratory of Brain Science and Medicine, School of Life Science and Technology, Southeast University","place":["Nanjing, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4217-2367","authenticated-orcid":false,"given":"Sheng","family":"Li","sequence":"additional","affiliation":[{"name":"Guangdong Provincial Key Laboratory of Insect Developmental Biology and Applied Technology, Institute of Insect Science and Technology and School of Life Sciences, South China Normal University","place":["Guangzhou, China"]},{"name":"Guangmeiyuan R&D Center, Guangdong Provincial Key Laboratory of Insect Developmental Biology and Applied Technology, South China Normal University","place":["Meizhou, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7738-6218","authenticated-orcid":false,"given":"Suning","family":"Liu","sequence":"additional","affiliation":[{"name":"Guangdong Provincial Key Laboratory of Insect Developmental Biology and Applied Technology, Institute of Insect Science and Technology and School of Life Sciences, South China Normal University","place":["Guangzhou, China"]},{"name":"Guangmeiyuan R&D Center, Guangdong Provincial Key Laboratory of Insect Developmental Biology and Applied Technology, South China Normal University","place":["Meizhou, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2287-1363","authenticated-orcid":false,"given":"Qionglin","family":"Peng","sequence":"additional","affiliation":[{"name":"The Key Laboratory of Developmental Genes and Human Disease, Jiangsu Key Laboratory of Brain Science and Medicine, School of Life Science and Technology, Southeast University","place":["Nanjing, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1535-9716","authenticated-orcid":false,"given":"Yufeng","family":"Pan","sequence":"additional","affiliation":[{"name":"The Key Laboratory of Developmental Genes and Human Disease, Jiangsu Key Laboratory of Brain Science and Medicine, School of Life Science and Technology, Southeast University","place":["Nanjing, China"]},{"name":"Co-innovation Center of Neuroregeneration, Nantong University","place":["Nantong, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"341","published-online":{"date-parts":[[2025,2,21]]},"reference":[{"key":"e_1_3_4_1_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.yfrne.2015.04.002"},{"key":"e_1_3_4_2_2","doi-asserted-by":"publisher","DOI":"10.3389\/fendo.2020.602285"},{"key":"e_1_3_4_3_2","doi-asserted-by":"publisher","DOI":"10.3109\/03014460.2012.693199"},{"key":"e_1_3_4_4_2","doi-asserted-by":"publisher","DOI":"10.1210\/endo-65-3-369"},{"key":"e_1_3_4_5_2","doi-asserted-by":"publisher","DOI":"10.1038\/nn.2834"},{"key":"e_1_3_4_6_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.yhbeh.2009.03.010"},{"key":"e_1_3_4_7_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.yhbeh.2009.03.011"},{"key":"e_1_3_4_8_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.neubiorev.2016.07.036"},{"key":"e_1_3_4_9_2","doi-asserted-by":"publisher","DOI":"10.1016\/0018-506X(77)90006-X"},{"key":"e_1_3_4_10_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1716897115"},{"key":"e_1_3_4_11_2","doi-asserted-by":"publisher","DOI":"10.1126\/sciadv.abg6926"},{"key":"e_1_3_4_12_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1318119110"},{"key":"e_1_3_4_13_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-022-28592-2"},{"key":"e_1_3_4_14_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1620760114"},{"key":"e_1_3_4_15_2","doi-asserted-by":"publisher","DOI":"10.15252\/embr.202356898"},{"key":"e_1_3_4_16_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0055131"},{"key":"e_1_3_4_17_2","doi-asserted-by":"publisher","DOI":"10.1186\/s40851-015-0031-2"},{"key":"e_1_3_4_18_2","doi-asserted-by":"publisher","DOI":"10.1007\/s40778-017-0070-z"},{"key":"e_1_3_4_19_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pgen.1006788"},{"key":"e_1_3_4_20_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pbio.3000145"},{"key":"e_1_3_4_21_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.ydbio.2024.01.004"},{"key":"e_1_3_4_22_2","doi-asserted-by":"publisher","DOI":"10.1266\/ggs.89.27"},{"key":"e_1_3_4_23_2","doi-asserted-by":"publisher","DOI":"10.1242\/bio.029744"},{"key":"e_1_3_4_24_2","doi-asserted-by":"publisher","DOI":"10.59717\/j.xinn-life.2024.100060"},{"key":"e_1_3_4_25_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.cub.2009.06.063"},{"key":"e_1_3_4_26_2","doi-asserted-by":"publisher","DOI":"10.3390\/biology10070614"},{"key":"e_1_3_4_27_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbagrm.2014.05.025"},{"key":"e_1_3_4_28_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.cois.2018.07.003"},{"key":"e_1_3_4_29_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1614383114"},{"key":"e_1_3_4_30_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.stem.2010.10.001"},{"key":"e_1_3_4_31_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.2310841121"},{"key":"e_1_3_4_32_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.neuron.2014.05.038"},{"key":"e_1_3_4_33_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41586-020-2055-9"},{"key":"e_1_3_4_34_2","doi-asserted-by":"publisher","DOI":"10.1007\/s12264-022-00886-x"},{"key":"e_1_3_4_35_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41586-020-2972-7"},{"key":"e_1_3_4_36_2","doi-asserted-by":"publisher","DOI":"10.1016\/0003-3472(67)90006-1"},{"key":"e_1_3_4_37_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.neuron.2019.01.045"},{"key":"e_1_3_4_38_2","doi-asserted-by":"publisher","DOI":"10.3390\/ijms22041940"},{"key":"e_1_3_4_39_2","doi-asserted-by":"publisher","DOI":"10.1002\/cne.10555"},{"key":"e_1_3_4_40_2","doi-asserted-by":"publisher","DOI":"10.1007\/s00441-009-0890-y"},{"key":"e_1_3_4_41_2","doi-asserted-by":"publisher","DOI":"10.1038\/nature07001"},{"key":"e_1_3_4_42_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2013.11.045"},{"key":"e_1_3_4_43_2","doi-asserted-by":"publisher","DOI":"10.1007\/s00018-017-2682-y"},{"key":"e_1_3_4_44_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41593-020-0668-9"},{"key":"e_1_3_4_45_2","doi-asserted-by":"publisher","DOI":"10.3389\/fphys.2021.785320"},{"key":"e_1_3_4_46_2","doi-asserted-by":"publisher","DOI":"10.1016\/0092-8674(93)90359-X"},{"key":"e_1_3_4_47_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jinsphys.2012.01.013"},{"key":"e_1_3_4_48_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M203694200"},{"key":"e_1_3_4_49_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.neuron.2014.05.017"},{"key":"e_1_3_4_50_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.neuron.2009.01.009"},{"key":"e_1_3_4_51_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.neuron.2008.12.021"},{"key":"e_1_3_4_52_2","doi-asserted-by":"publisher","DOI":"10.1038\/ncomms10024"},{"key":"e_1_3_4_53_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.cub.2021.02.048"},{"key":"e_1_3_4_54_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.ibmb.2003.06.007"},{"key":"e_1_3_4_55_2","doi-asserted-by":"publisher","DOI":"10.1007\/BF01066724"},{"key":"e_1_3_4_56_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.neuron.2016.05.004"},{"key":"e_1_3_4_57_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0151912"},{"key":"e_1_3_4_58_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0083-6729(00)60016-X"},{"key":"e_1_3_4_59_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.2208935119"},{"key":"e_1_3_4_60_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1900343116"},{"key":"e_1_3_4_61_2","doi-asserted-by":"publisher","DOI":"10.1242\/dev.126.20.4581"},{"key":"e_1_3_4_62_2","doi-asserted-by":"publisher","DOI":"10.1093\/genetics\/154.3.1203"},{"key":"e_1_3_4_63_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-020-17771-8"},{"key":"e_1_3_4_64_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41593-019-0515-z"},{"key":"e_1_3_4_65_2","doi-asserted-by":"publisher","DOI":"10.7554\/eLife.19334"},{"key":"e_1_3_4_66_2","doi-asserted-by":"publisher","DOI":"10.1038\/nn.4263"},{"key":"e_1_3_4_67_2","doi-asserted-by":"publisher","DOI":"10.1002\/cne.23768"},{"key":"e_1_3_4_68_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.cub.2010.04.039"},{"key":"e_1_3_4_69_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.2104461118"},{"key":"e_1_3_4_70_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41592-019-0435-6"},{"key":"e_1_3_4_71_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.neuron.2022.04.010"},{"key":"e_1_3_4_72_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1417838112"},{"key":"e_1_3_4_73_2","doi-asserted-by":"publisher","DOI":"10.1534\/genetics.109.103630"},{"key":"e_1_3_4_74_2","doi-asserted-by":"publisher","DOI":"10.7554\/eLife.08477"},{"key":"e_1_3_4_75_2","doi-asserted-by":"publisher","DOI":"10.1038\/nmeth.2836"},{"key":"e_1_3_4_76_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1405500111"},{"key":"e_1_3_4_77_2","doi-asserted-by":"publisher","DOI":"10.7554\/eLife.59224"},{"key":"e_1_3_4_78_2","doi-asserted-by":"publisher","DOI":"10.3390\/cells10061454"}],"container-title":["Proceedings of the National Academy of Sciences"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/pnas.org\/doi\/pdf\/10.1073\/pnas.2418481122","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,2,21]],"date-time":"2025-02-21T11:37:09Z","timestamp":1740137829000},"score":1,"resource":{"primary":{"URL":"https:\/\/pnas.org\/doi\/10.1073\/pnas.2418481122"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,2,21]]},"references-count":78,"journal-issue":{"issue":"8","published-print":{"date-parts":[[2025,2,25]]}},"alternative-id":["10.1073\/pnas.2418481122"],"URL":"https:\/\/doi.org\/10.1073\/pnas.2418481122","relation":{},"ISSN":["0027-8424","1091-6490"],"issn-type":[{"value":"0027-8424","type":"print"},{"value":"1091-6490","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025,2,21]]},"assertion":[{"value":"2024-09-10","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2025-01-21","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2025-02-21","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e2418481122"}}