{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,30]],"date-time":"2026-08-30T05:33:44Z","timestamp":1788068024306,"version":"build-2784847793"},"reference-count":57,"publisher":"National Academy of Sciences","issue":"24","license":[{"start":{"date-parts":[[2025,6,10]],"date-time":"2025-06-10T00:00:00Z","timestamp":1749513600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001691","name":"MEXT | Japan Society for the Promotion of Science","doi-asserted-by":"publisher","award":["17H03686"],"award-info":[{"award-number":["17H03686"]}],"id":[{"id":"10.13039\/501100001691","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001691","name":"MEXT | Japan Society for the Promotion of Science","doi-asserted-by":"publisher","award":["21H02489"],"award-info":[{"award-number":["21H02489"]}],"id":[{"id":"10.13039\/501100001691","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001691","name":"MEXT | Japan Society for the Promotion of Science","doi-asserted-by":"publisher","award":["23H00364"],"award-info":[{"award-number":["23H00364"]}],"id":[{"id":"10.13039\/501100001691","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["www.pnas.org"],"crossmark-restriction":true},"short-container-title":["Proc. Natl. Acad. Sci. U.S.A."],"published-print":{"date-parts":[[2025,6,17]]},"abstract":"<jats:p>\n                    Germ cells transmit genetic information to offspring and maintain the genome of the species. In many animals including\n                    <jats:italic toggle=\"yes\">Drosophila<\/jats:italic>\n                    , germ cell formation relies on maternal determinants in the germ plasm. Several proteins present in the germ plasm of oocytes also localize to the perinuclear nuage in nurse cells, where they contribute to the production of PIWI-interacting RNAs (piRNAs). These piRNAs guide the silencing of transposons, thereby protecting the germline genome from invading mobile elements. Aubergine (Aub) is a germ plasm\/nuage protein and a piRNA-directed endonuclease that inactivates transposons. Aub is also essential for germ plasm assembly. The Aub-bound piRNAs in the germ plasm are inherited by the progeny germline and serve as templates for piRNA biogenesis in the next generation. Thus, piRNA production in the nurse cell nuage is thought to be coordinated with germ plasm assembly in the oocyte. However, the underlying mechanism remains unclear. Here, we report that a maternal factor, named\n                    <jats:italic toggle=\"yes\">tiny pole plasm<\/jats:italic>\n                    (\n                    <jats:italic toggle=\"yes\">tpp<\/jats:italic>\n                    ), mediates this coordination. Tpp is a GTSF1 family PIWI cofactor. In\n                    <jats:italic toggle=\"yes\">\n                      tpp\n                      <jats:sup>\u2212<\/jats:sup>\n                    <\/jats:italic>\n                    ovaries, the production of piRNAs, particularly Aub-bound piRNAs, is defective, resulting in reduced Aub localization to the germ plasm and impaired germ cell formation. Notably, the levels of piRNA production required for proper Aub localization are much higher than those required for transposon silencing. We propose that producing abundant piRNAs beyond what is required for transposon silencing in the ovary promotes germ plasm assembly, thereby enabling the progeny germline to properly silence transposons for species survival.\n                  <\/jats:p>","DOI":"10.1073\/pnas.2419375122","type":"journal-article","created":{"date-parts":[[2025,6,10]],"date-time":"2025-06-10T11:07:53Z","timestamp":1749553673000},"update-policy":"https:\/\/doi.org\/10.1073\/pnas.cm10313","source":"Crossref","is-referenced-by-count":9,"title":["Abundant piRNA production mediated by the\n                    <i>Drosophila<\/i>\n                    GTSF1 homolog Tpp ensures Aubergine localization and germ plasm assembly"],"prefix":"10.1073","volume":"122","author":[{"ORCID":"https:\/\/orcid.org\/0009-0004-7192-9009","authenticated-orcid":false,"given":"Hirono","family":"Kina","sequence":"first","affiliation":[{"name":"Department of Germline Development, Institute of Molecular Embryology and Genetics, Kumamoto University","place":["Kumamoto, Japan"]},{"name":"Department of Germline Development, Graduate School of Pharmaceutical Sciences, Kumamoto University","place":["Kumamoto, Japan"]},{"name":"Laboratory of RNA Function, Institute for Quantitative Biosciences, The University of Tokyo","place":["Tokyo, Japan"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Natsuko","family":"Izumi","sequence":"additional","affiliation":[{"name":"Laboratory of RNA Function, Institute for Quantitative Biosciences, The University of Tokyo","place":["Tokyo, Japan"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kazuko","family":"Hanyu-Nakamura","sequence":"additional","affiliation":[{"name":"Department of Germline Development, Institute of Molecular Embryology and Genetics, Kumamoto University","place":["Kumamoto, Japan"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Takashi","family":"Yoshitani","sequence":"additional","affiliation":[{"name":"Department of Germline Development, Institute of Molecular Embryology and Genetics, Kumamoto University","place":["Kumamoto, Japan"]},{"name":"Department of Germline Development, School of Pharmacy, Kumamoto University","place":["Kumamoto, Japan"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8354-174X","authenticated-orcid":false,"given":"Mariko","family":"Yamane","sequence":"additional","affiliation":[{"name":"Department of Pluripotent Stem Cell Biology, Institute of Molecular Embryology and Genetics, Kumamoto University","place":["Kumamoto, Japan"]},{"name":"Laboratory for Bioinformatics Research, RIKEN Center for Biosystems Dynamic Research","place":["Kobe, Japan"]},{"name":"Department of Functional Genome Informatics, Medical Research Laboratory, Institute of Integrated Research, Institute of Science Tokyo","place":["Tokyo, Japan"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hitoshi","family":"Niwa","sequence":"additional","affiliation":[{"name":"Department of Pluripotent Stem Cell Biology, Institute of Molecular Embryology and Genetics, Kumamoto University","place":["Kumamoto, Japan"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8442-0851","authenticated-orcid":false,"given":"Yukihide","family":"Tomari","sequence":"additional","affiliation":[{"name":"Laboratory of RNA Function, Institute for Quantitative Biosciences, The University of Tokyo","place":["Tokyo, Japan"]},{"name":"Department of Computational Biology and Medical Sciences, Graduate School of Frontier Sciences, The University of Tokyo","place":["Bunkyo-ku, Japan"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6506-9146","authenticated-orcid":false,"given":"Akira","family":"Nakamura","sequence":"additional","affiliation":[{"name":"Department of Germline Development, Institute of Molecular Embryology and Genetics, Kumamoto University","place":["Kumamoto, Japan"]},{"name":"Department of Germline Development, Graduate School of Pharmaceutical Sciences, Kumamoto University","place":["Kumamoto, 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