{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,20]],"date-time":"2026-10-20T07:01:08Z","timestamp":1792479668348,"version":"4.5.0"},"reference-count":69,"publisher":"National Academy of Sciences","issue":"8","license":[{"start":{"date-parts":[[2025,2,18]],"date-time":"2025-02-18T00:00:00Z","timestamp":1739836800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"funder":[{"DOI":"10.13039\/100000057","name":"HHS | NIH | National Institute of General Medical Sciences","doi-asserted-by":"publisher","award":["R35 GM122471"],"award-info":[{"award-number":["R35 GM122471"]}],"id":[{"id":"10.13039\/100000057","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["www.pnas.org"],"crossmark-restriction":true},"short-container-title":["Proc. Natl. Acad. Sci. U.S.A."],"published-print":{"date-parts":[[2025,2,25]]},"abstract":"<jats:p>\n                    The often-distinctive pigment patterns of vertebrates are varied in form and function and depend on several types of pigment cells derived from embryonic neural crest or latent stem cells of neural crest origin. These cells and the patterns they produce have been useful for uncovering features of differentiation and morphogenesis that underlie adult phenotypes, and they offer opportunities to discover how patterns and the cell types themselves have diversified. In zebrafish, a body pattern of stripes arises by self-organizing interactions among three types of pigment cells. Yet these fish also exhibit white ornamentation on their fins that depends on the transdifferentiation of black melanophores to white cells, \u201cmelanoleucophores.\u201d To identify mechanisms underlying this conversion we used ultrastructural, transcriptomic, mutational, and other approaches. We show that melanophore\u2013melanoleucophore transition depends on regional BMP signals transduced through noncanonical receptors (Rgmb-Neo1a-Lrig2) as well as BMP-dependent signaling by Agouti genes,\n                    <jats:italic>asip1<\/jats:italic>\n                    and\n                    <jats:italic>asip2b<\/jats:italic>\n                    . These signals lead to expression of transcription factor genes including\n                    <jats:italic>foxd3<\/jats:italic>\n                    and\n                    <jats:italic>runx3<\/jats:italic>\n                    that are necessary to induce loss of melanin, curtail new melanin production, and deploy a pathway for accumulating guanine crystals that, together, confer a white phenotype. These analyses uncover an important role for positional information in specifying ornamentation in zebrafish and show how tissue environmental cues and an altered gene regulatory program have allowed terminal addition of a distinct phenotype to a preexisting cell type.\n                  <\/jats:p>","DOI":"10.1073\/pnas.2424180122","type":"journal-article","created":{"date-parts":[[2025,2,18]],"date-time":"2025-02-18T11:40:41Z","timestamp":1739878841000},"update-policy":"https:\/\/doi.org\/10.1073\/pnas.cm10313","source":"Crossref","is-referenced-by-count":15,"title":["Agouti and BMP signaling drive a naturally occurring fate conversion of melanophores to leucophores in zebrafish"],"prefix":"10.1073","volume":"122","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-3966-6462","authenticated-orcid":false,"given":"Delai","family":"Huang","sequence":"first","affiliation":[{"name":"Department of Biology, University of Virginia","place":["Charlottesville"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Emaan H.","family":"Kapadia","sequence":"additional","affiliation":[{"name":"Department of Biology, University of Virginia","place":["Charlottesville"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1664-7864","authenticated-orcid":false,"given":"Yipeng","family":"Liang","sequence":"additional","affiliation":[{"name":"Department of Biology, University of Virginia","place":["Charlottesville"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Leah P.","family":"Shriver","sequence":"additional","affiliation":[{"name":"Department of Chemistry, Washington University","place":["St. Louis"]},{"name":"The Center for Mass Spectrometry and Isotope Tracing, Washington University","place":["St. Louis"]},{"name":"Department of Medicine, Washington University School of Medicine","place":["St. Louis"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5345-7053","authenticated-orcid":false,"given":"Shengkun","family":"Dai","sequence":"additional","affiliation":[{"name":"Department of Chemistry, Washington University","place":["St. Louis"]},{"name":"The Center for Mass Spectrometry and Isotope Tracing, Washington University","place":["St. Louis"]},{"name":"Department of Medicine, Washington University School of Medicine","place":["St. Louis"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gary J.","family":"Patti","sequence":"additional","affiliation":[{"name":"Department of Chemistry, Washington University","place":["St. Louis"]},{"name":"The Center for Mass Spectrometry and Isotope Tracing, Washington University","place":["St. Louis"]},{"name":"Department of Medicine, Washington University School of Medicine","place":["St. Louis"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bruno M.","family":"Humbel","sequence":"additional","affiliation":[{"name":"Marine Eco-Evo-Devo Unit, Okinawa Institute of Science and Technology Graduate University","place":["Okinawa, Japan"]},{"name":"Provost Office, Okinawa Institute of Science and Technology Graduate University","place":["Okinawa, Japan"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Vincent","family":"Laudet","sequence":"additional","affiliation":[{"name":"Marine Eco-Evo-Devo Unit, Okinawa Institute of Science and Technology Graduate University","place":["Okinawa, Japan"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2771-6095","authenticated-orcid":false,"given":"David M.","family":"Parichy","sequence":"additional","affiliation":[{"name":"Department of Biology, University of Virginia","place":["Charlottesville"]},{"name":"Department of Cell Biology, University of 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Huang D. M. Parichy Data from \u201cAgouti and BMP signaling drive a naturally occurring fate conversion of melanophores to leucophores in zebrafish.\u201d NCBI GEO. https:\/\/www.ncbi.nlm.nih.gov\/geo\/query\/acc.cgi?acc=GSE282061. Deposited 15 November 2024.","DOI":"10.1101\/2024.11.20.624586"}],"container-title":["Proceedings of the National Academy of Sciences"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/pnas.org\/doi\/pdf\/10.1073\/pnas.2424180122","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,2,18]],"date-time":"2025-02-18T11:41:42Z","timestamp":1739878902000},"score":1,"resource":{"primary":{"URL":"https:\/\/pnas.org\/doi\/10.1073\/pnas.2424180122"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,2,18]]},"references-count":69,"journal-issue":{"issue":"8","published-print":{"date-parts":[[2025,2,25]]}},"alternative-id":["10.1073\/pnas.2424180122"],"URL":"https:\/\/doi.org\/10.1073\/pnas.2424180122","relation":{"has-preprint":[{"id-type":"doi","id":"10.1101\/2024.11.20.624586","asserted-by":"object"}]},"ISSN":["0027-8424","1091-6490"],"issn-type":[{"value":"0027-8424","type":"print"},{"value":"1091-6490","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025,2,18]]},"assertion":[{"value":"2024-11-22","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2025-01-17","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2025-02-18","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e2424180122"}}