{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,12]],"date-time":"2026-10-12T23:06:05Z","timestamp":1791846365716,"version":"4.3.4"},"reference-count":53,"publisher":"National Academy of Sciences","issue":"12","license":[{"start":{"date-parts":[[2025,3,17]],"date-time":"2025-03-17T00:00:00Z","timestamp":1742169600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"funder":[{"DOI":"10.13039\/100000060","name":"HHS | NIH | National Institute of Allergy and Infectious Diseases","doi-asserted-by":"publisher","award":["R01AI145862"],"award-info":[{"award-number":["R01AI145862"]}],"id":[{"id":"10.13039\/100000060","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["www.pnas.org"],"crossmark-restriction":true},"short-container-title":["Proc. Natl. Acad. Sci. U.S.A."],"published-print":{"date-parts":[[2025,3,25]]},"abstract":"<jats:p>\n                    Maternal\u2013fetal immune tolerance guarantees a successful pregnancy throughout gestation. HLA-G, a nonclassical human leukocyte antigen (HLA) molecule exclusively expressed in extravillous trophoblasts (EVT), is a crucial factor in establishing maternal\u2013fetal immune tolerance by interacting with inhibitory receptors on various maternal immune cells residing in the uterus. While trophoblast-specific cis-regulatory elements impacting\n                    <jats:italic>HLA-G<\/jats:italic>\n                    transcription have been described, the identity of trans-acting factors controlling\n                    <jats:italic>HLA-G<\/jats:italic>\n                    expression in EVT remains poorly understood. Utilizing a genome-wide CRISPR-Cas9 knockout screen, we find that the WNT signaling pathway negatively regulates\n                    <jats:italic>HLA-G<\/jats:italic>\n                    expression in EVT. In addition, we identified two trophoblast-specific transcription factors, TEAD1 and TEAD3, required for\n                    <jats:italic>HLA-G<\/jats:italic>\n                    transcription in EVT in a Yes-associated protein-independent manner. Altogether, we systematically elucidated essential genes and pathways underlying HLA-G expression in EVT, shedding light on the mechanisms of maternal\u2013fetal tolerance and potentially providing insights into controlling HLA-G expression beyond EVT to protect allogeneic cells from immune rejection.\n                  <\/jats:p>","DOI":"10.1073\/pnas.2425339122","type":"journal-article","created":{"date-parts":[[2025,3,17]],"date-time":"2025-03-17T15:32:56Z","timestamp":1742225576000},"update-policy":"https:\/\/doi.org\/10.1073\/pnas.cm10313","source":"Crossref","is-referenced-by-count":13,"title":["The TEA domain transcription factors TEAD1 and TEAD3 and WNT signaling determine HLA-G expression in human extravillous trophoblasts"],"prefix":"10.1073","volume":"122","author":[{"given":"Bowen","family":"Gu","sequence":"first","affiliation":[{"name":"Department of Stem Cell and Regenerative Biology, Harvard University","place":["Cambridge"]},{"name":"Program in Cellular and Molecular Medicine Boston Children\u2019s Hospital","place":["Boston"]},{"name":"Department of Pediatrics, Harvard Medical School","place":["Boston"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Leonardo M. 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