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Natl. Acad. Sci. U.S.A."],"published-print":{"date-parts":[[2025,3,11]]},"abstract":"<jats:p>Sperm IZUMO1 binds to egg JUNO, and this interaction is essential for mammalian fertilization. Isolated from a female mouse immunized with syngeneic sperm, the antisperm antibody OBF13 recognizes IZUMO1 and inhibits murine fertilization. How OBF13 interferes with sperm\u2013egg interactions was unknown. Here, we present the X-ray crystal structure of IZUMO1 in complex with OBF13. OBF13 binds to the apex of the four-helix domain of IZUMO1, distant from the JUNO-binding site. Our crystal structure of OBF13-bound IZUMO1 resembles apo-IZUMO1 and differs from the structure of IZUMO1 in complex with JUNO. We identify that OBF13 carries a low level of somatic hypermutation, and through deep mutational scanning, we engineer an affinity-enhanced OBF13 variant. This OBF13 variant single-chain fragment variable decreases the apparent affinity of IZUMO1 for membrane-bound murine JUNO and blocks the binding of acrosome-reacted sperm to eggs, thereby preventing fertilization. We propose allostery between the OBF13 epitope and the JUNO-binding site. OBF13 inhibits a conformational change in IZUMO1, preventing fusion-competent sperm from adhering to murine eggs during fertilization. Surprisingly, murine IZUMO1 binds to hamster JUNO with an affinity ~20-fold higher than to murine JUNO. The decreased affinity caused by OBF13 of murine IZUMO1 for hamster JUNO is sufficient for murine sperm to bind to and fuse with hamster eggs. Our studies provide a structural and mechanistic framework for species-specific, allosteric inhibition of IZUMO1 by a naturally occurring antisperm antibody and offer insights into the development of immunocontraceptives.<\/jats:p>","DOI":"10.1073\/pnas.2425952122","type":"journal-article","created":{"date-parts":[[2025,3,5]],"date-time":"2025-03-05T11:45:21Z","timestamp":1741175121000},"update-policy":"https:\/\/doi.org\/10.1073\/pnas.cm10313","source":"Crossref","is-referenced-by-count":5,"title":["Allosteric inhibition of the IZUMO1\u2013JUNO fertilization complex by the naturally occurring antisperm antibody OBF13"],"prefix":"10.1073","volume":"122","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-0198-8906","authenticated-orcid":false,"given":"Yonggang","family":"Lu","sequence":"first","affiliation":[{"name":"Premium Research Institute for Human Metaverse Medicine, Osaka University","place":["Suita,, Japan"]},{"name":"Research Institute for Microbial Diseases, Osaka University","place":["Suita,, Japan"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9859-6217","authenticated-orcid":false,"given":"Masahito","family":"Ikawa","sequence":"additional","affiliation":[{"name":"Research Institute for Microbial Diseases, Osaka University","place":["Suita,, Japan"]},{"name":"The Institute of Medical Science, The University of Tokyo","place":["Minato-ku,, Japan"]},{"name":"Center for Infectious Disease Education and Research, Osaka University","place":["Suita,, Japan"]},{"name":"Center for Advanced Modalities and Drug Delivery System, Osaka University","place":["Suita,, Japan"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3904-492X","authenticated-orcid":false,"given":"Shaogeng","family":"Tang","sequence":"additional","affiliation":[{"name":"Department of Molecular Biophysics and Biochemistry, Yale University","place":["New Haven"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"341","published-online":{"date-parts":[[2025,3,5]]},"reference":[{"key":"e_1_3_4_1_2","doi-asserted-by":"publisher","DOI":"10.1038\/nature03362"},{"key":"e_1_3_4_2_2","doi-asserted-by":"publisher","DOI":"10.1038\/nature13203"},{"key":"e_1_3_4_3_2","doi-asserted-by":"publisher","DOI":"10.1007\/s00335-013-9491-x"},{"key":"e_1_3_4_4_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1922650117"},{"key":"e_1_3_4_5_2","doi-asserted-by":"publisher","DOI":"10.7554\/eLife.53913"},{"key":"e_1_3_4_6_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-020-62091-y"},{"key":"e_1_3_4_7_2","doi-asserted-by":"publisher","DOI":"10.1038\/s42003-022-03289-w"},{"key":"e_1_3_4_8_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pgen.1004044"},{"key":"e_1_3_4_9_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.2207805119"},{"key":"e_1_3_4_10_2","doi-asserted-by":"publisher","DOI":"10.7554\/eLife.66313"},{"key":"e_1_3_4_11_2","doi-asserted-by":"publisher","DOI":"10.7554\/eLife.93131"},{"key":"e_1_3_4_12_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2024.09.035"},{"key":"e_1_3_4_13_2","doi-asserted-by":"publisher","DOI":"10.1016\/0165-0378(86)90026-4"},{"key":"e_1_3_4_14_2","doi-asserted-by":"publisher","DOI":"10.1016\/0165-0378(87)90014-3"},{"key":"e_1_3_4_15_2","doi-asserted-by":"publisher","DOI":"10.1016\/0165-0378(89)90007-7"},{"key":"e_1_3_4_16_2","doi-asserted-by":"publisher","DOI":"10.1016\/0165-0378(88)90002-2"},{"key":"e_1_3_4_17_2","first-page":"473","article-title":"An anti-mouse sperm monoclonal antibody (OBF13) and its inhibitory activity on fertilization","volume":"33","author":"Okabe M.","year":"1988","unstructured":"M. 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