{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,27]],"date-time":"2026-10-27T00:16:56Z","timestamp":1793060216903,"version":"4.5.0"},"reference-count":38,"publisher":"National Academy of Sciences","issue":"30","license":[{"start":{"date-parts":[[2025,7,23]],"date-time":"2025-07-23T00:00:00Z","timestamp":1753228800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"funder":[{"DOI":"10.13039\/100010269","name":"Wellcome Trust","doi-asserted-by":"publisher","award":["219666\/Z\/19\/Z"],"award-info":[{"award-number":["219666\/Z\/19\/Z"]}],"id":[{"id":"10.13039\/100010269","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["www.pnas.org"],"crossmark-restriction":true},"short-container-title":["Proc. Natl. Acad. Sci. U.S.A."],"published-print":{"date-parts":[[2025,7,29]]},"abstract":"<jats:p>\n                    Shigellosis is a global public health challenge that mostly affects low- and middle-income countries and causes considerable morbidity and mortality among children under 5 y of age. Multi- and extensively drug-resistant\n                    <jats:italic toggle=\"yes\">Shigella sonnei<\/jats:italic>\n                    strains associated with recent outbreaks in high-income countries exacerbate the problem and have prompted the World Health Organization to include\n                    <jats:italic toggle=\"yes\">Shigella<\/jats:italic>\n                    spp. among the high-risk pathogens for which novel prophylactic and therapeutic tools are urgently needed. Among the most promising and cutting-edge solutions, monoclonal antibodies are gaining considerable attention in the infectious diseases field. Here, we report the discovery of human monoclonal antibodies against\n                    <jats:italic toggle=\"yes\">S. sonnei<\/jats:italic>\n                    , a species whose prevalence is constantly increasing worldwide and is associated with frequent drug-resistant infections. We isolated antibodies generated in response to an experimental\n                    <jats:italic toggle=\"yes\">S. sonnei<\/jats:italic>\n                    vaccine followed by a controlled human infection and screened them by using a panel of high-throughput assays. We identified a molecule which exhibited potent bactericidal activity in vitro, inhibition of invasion of epithelial cells and conferred full protection from\n                    <jats:italic toggle=\"yes\">S. sonnei<\/jats:italic>\n                    infection in vivo. Overall, our study provides a candidate antibody that can rapidly progress to industrial development for application as a prophylactic, therapeutic, and diagnostic tool against shigellosis.\n                  <\/jats:p>","DOI":"10.1073\/pnas.2426211122","type":"journal-article","created":{"date-parts":[[2025,7,23]],"date-time":"2025-07-23T16:09:09Z","timestamp":1753286949000},"update-policy":"https:\/\/doi.org\/10.1073\/pnas.cm10313","source":"Crossref","is-referenced-by-count":6,"title":["A multifunctional anti-O-Antigen human monoclonal antibody protects against\n                    <i>Shigella sonnei<\/i>\n                    infection in vivo"],"prefix":"10.1073","volume":"122","author":[{"given":"Matteo","family":"Ridelfi","sequence":"first","affiliation":[{"name":"Monoclonal Antibody Discovery Laboratory, Fondazione Toscana Life Sciences","place":["Siena, Italy"]},{"name":"Department of Biotechnology, Chemistry and Pharmacy, Universit\u00e0 di Siena","place":["Siena, Italy"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Giacomo","family":"Vezzani","sequence":"additional","affiliation":[{"name":"GSK Vaccines Institute for Global Health","place":["Siena, Italy"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Emanuele","family":"Roscioli","sequence":"additional","affiliation":[{"name":"Monoclonal Antibody Discovery Laboratory, Fondazione Toscana Life Sciences","place":["Siena, Italy"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Giampiero","family":"Batani","sequence":"additional","affiliation":[{"name":"Monoclonal Antibody Discovery Laboratory, Fondazione Toscana Life Sciences","place":["Siena, Italy"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Elena","family":"Boero","sequence":"additional","affiliation":[{"name":"GSK Vaccines Institute for Global Health","place":["Siena, 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