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This localization is regulated by the small GTPase RAP1, independently of known lateral (G\u03b1i, GPSM2) or medial (aPKC\u03b6, PARD6B) proteins. Hair cell-specific ablation of\n                    <jats:italic toggle=\"yes\">Shank2<\/jats:italic>\n                    or\n                    <jats:italic toggle=\"yes\">Rap1<\/jats:italic>\n                    disrupts bundle architecture while preserving key features essential for mechanotransduction. In particular, OHCs lose their unique bundle geometry and show impaired amplification, especially at high frequencies. Longitudinal studies further reveal that this architectural disruption leads to progressive bundle degeneration and hearing loss. These findings suggest that the characteristic bundle architecture, particularly the V-shaped geometry of OHCs, is essential for high-frequency hearing and long-term bundle integrity in the mammalian cochlea.\n                  <\/jats:p>","DOI":"10.1073\/pnas.2426646122","type":"journal-article","created":{"date-parts":[[2025,7,8]],"date-time":"2025-07-08T11:39:52Z","timestamp":1751974792000},"update-policy":"https:\/\/doi.org\/10.1073\/pnas.cm10313","source":"Crossref","is-referenced-by-count":2,"title":["SHANK2 establishes auditory hair bundle architecture essential for mammalian hearing"],"prefix":"10.1073","volume":"122","author":[{"ORCID":"https:\/\/orcid.org\/0009-0008-0980-9865","authenticated-orcid":false,"given":"Han Seul","family":"Choi","sequence":"first","affiliation":[{"name":"Department of Anatomy, Yonsei University College of Medicine","place":["Seoul, Korea"]},{"name":"Brain Korea 21 project for Medical Science, Yonsei University College of Medicine","place":["Seoul, 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