{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,10]],"date-time":"2026-06-10T15:30:41Z","timestamp":1781105441740,"version":"3.54.1"},"reference-count":52,"publisher":"National Academy of Sciences","issue":"26","content-domain":{"domain":["www.pnas.org"],"crossmark-restriction":true},"short-container-title":["Proc. Natl. Acad. Sci. U.S.A."],"published-print":{"date-parts":[[2003,12,23]]},"abstract":"<jats:p>\n                    Neurological symptoms are common manifestations of Lyme disease; however, the paucibacillary nature of the spirochete in this environment has precluded a molecular analysis of the spirochete in the CNS. We have now adapted differential expression analysis by using a custom-amplified library (DECAL) in conjunction with\n                    <jats:italic>Borrelia burgdorferi<\/jats:italic>\n                    whole-genome and subgenome arrays to examine\n                    <jats:italic>in vivo<\/jats:italic>\n                    gene expression by\n                    <jats:italic>B. burgdorferi<\/jats:italic>\n                    in a non-human primate (NHP) model of neuroborreliosis. The expression profile of\n                    <jats:italic>B. burgdorferi<\/jats:italic>\n                    was examined in the CNS and heart of steroid-treated and immunocompetent NHPs. Eighty-six chromosomal genes and 80 plasmid-encoded genes were expressed at similar levels in the CNS and heart tissue of both immunocompetent and steroid-treated NHPs. The expression of 66 chromosomal genes and 32 plasmid-encoded genes was increased in the CNS of both immunocompetent and steroid-treated NHPs. It is likely that the expression of these genes is governed by physiological factors specific to the CNS milieu. However, 83 chromosomal and 114 plasmid-encoded genes showed contrasting expression profiles in steroid-treated and immunocompetent NHPs. The effect of dexamethasone on the immune status of the host as well as on the host metabolic pathways could contribute to these differences in the\n                    <jats:italic>B. burgdorferi<\/jats:italic>\n                    transcriptome. Results obtained herein underscore the complex interplay of host factors on\n                    <jats:italic>B. burgdorferi<\/jats:italic>\n                    gene expression\n                    <jats:italic>in vivo<\/jats:italic>\n                    . The results provide a global snapshot of the spirochetal transcriptome in the CNS and should spur the design of experiments aimed at understanding the molecular basis of neuroborreliosis.\n                  <\/jats:p>","DOI":"10.1073\/pnas.2432412100","type":"journal-article","created":{"date-parts":[[2003,12,23]],"date-time":"2003-12-23T12:58:11Z","timestamp":1072184291000},"page":"15953-15958","update-policy":"https:\/\/doi.org\/10.1073\/pnas.cm10313","source":"Crossref","is-referenced-by-count":45,"title":["<i>Borrelia burgdorferi<\/i>\n                    transcriptome in the central nervous system of non-human primates"],"prefix":"10.1073","volume":"100","author":[{"given":"Sukanya","family":"Narasimhan","sequence":"first","affiliation":[{"name":"Section of Rheumatology, Department of Internal Medicine, Yale University School of Medicine, New Haven, CT 06520; Department of Pathology and Center for Microbial Pathogenesis, and Departments of Medicine and Genetics and Developmental Biology, University of Connecticut Health Center, Farmington, CT 06030; Department of Bacteriology and Parasitology, Tulane National Primate Research Center, Tulane University Health Sciences Center, Covington, LA 70433; and Department of Neurosciences, University of..."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Melissa J.","family":"Camaino","sequence":"additional","affiliation":[{"name":"Section of Rheumatology, Department of Internal Medicine, Yale University School of Medicine, New Haven, CT 06520; Department of Pathology and Center for Microbial Pathogenesis, and Departments of Medicine and Genetics and Developmental Biology, University of Connecticut Health Center, Farmington, CT 06030; Department of Bacteriology and Parasitology, Tulane National Primate Research Center, Tulane University Health Sciences Center, Covington, LA 70433; and Department of Neurosciences, University of..."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fang Ting","family":"Liang","sequence":"additional","affiliation":[{"name":"Section of Rheumatology, Department of Internal Medicine, Yale University School of Medicine, New Haven, CT 06520; Department of Pathology and Center for Microbial Pathogenesis, and Departments of Medicine and Genetics and Developmental Biology, University of Connecticut Health Center, Farmington, CT 06030; Department of Bacteriology and Parasitology, Tulane National Primate Research Center, Tulane University Health Sciences Center, Covington, LA 70433; and Department of Neurosciences, University of..."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Felix","family":"Santiago","sequence":"additional","affiliation":[{"name":"Section of Rheumatology, Department of Internal Medicine, Yale University School of Medicine, New Haven, CT 06520; Department of Pathology and Center for Microbial Pathogenesis, and Departments of Medicine and Genetics and Developmental Biology, University of Connecticut Health Center, Farmington, CT 06030; Department of Bacteriology and Parasitology, Tulane National Primate Research Center, Tulane University Health Sciences Center, Covington, LA 70433; and Department of Neurosciences, University of..."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Michelle","family":"Laskowski","sequence":"additional","affiliation":[{"name":"Section of Rheumatology, Department of Internal Medicine, Yale University School of Medicine, New Haven, CT 06520; Department of Pathology and Center for Microbial Pathogenesis, and Departments of Medicine and Genetics and Developmental Biology, University of Connecticut Health Center, Farmington, CT 06030; Department of Bacteriology and Parasitology, Tulane National Primate Research Center, Tulane University Health Sciences Center, Covington, LA 70433; and Department of Neurosciences, University of..."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mario T.","family":"Philipp","sequence":"additional","affiliation":[{"name":"Section of Rheumatology, Department of Internal Medicine, Yale University School of Medicine, New Haven, CT 06520; Department of Pathology and Center for Microbial Pathogenesis, and Departments of Medicine and Genetics and Developmental Biology, University of Connecticut Health Center, Farmington, CT 06030; Department of Bacteriology and Parasitology, Tulane National Primate Research Center, Tulane University Health Sciences Center, Covington, LA 70433; and Department of Neurosciences, University of..."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Andrew R.","family":"Pachner","sequence":"additional","affiliation":[{"name":"Section of Rheumatology, Department of Internal Medicine, Yale University School of Medicine, New Haven, CT 06520; Department of Pathology and Center for Microbial Pathogenesis, and Departments of Medicine and Genetics and Developmental Biology, University of Connecticut Health Center, Farmington, CT 06030; Department of Bacteriology and Parasitology, Tulane National Primate Research Center, Tulane University Health Sciences Center, Covington, LA 70433; and Department of Neurosciences, University of..."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Justin D.","family":"Radolf","sequence":"additional","affiliation":[{"name":"Section of Rheumatology, Department of Internal Medicine, Yale University School of Medicine, New Haven, CT 06520; Department of Pathology and Center for Microbial Pathogenesis, and Departments of Medicine and Genetics and Developmental Biology, University of Connecticut Health Center, Farmington, CT 06030; Department of Bacteriology and Parasitology, Tulane National Primate Research Center, Tulane University Health Sciences Center, Covington, LA 70433; and Department of Neurosciences, University of..."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Erol","family":"Fikrig","sequence":"additional","affiliation":[{"name":"Section of Rheumatology, Department of Internal Medicine, Yale University School of Medicine, New Haven, CT 06520; 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