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Natl. Acad. Sci. U.S.A."],"published-print":{"date-parts":[[2025,7]]},"abstract":"<jats:p>\n                    Methane seeps harbor uncharacterized animal\u2013microbe symbioses with unique nutritional strategies. Three undescribed sea spider species (family Ammotheidae; genus\n                    <jats:italic toggle=\"yes\">Sericosura<\/jats:italic>\n                    ) endemic to methane seeps were found along the eastern Pacific margin, from California to Alaska, hosting diverse methane- and methanol-oxidizing bacteria on their exoskeleton. \u03b4\n                    <jats:sup>13<\/jats:sup>\n                    C tissue isotope values of in situ specimens corroborated methane assimilation (\u221245\u2030, on average). Live animal incubations with\n                    <jats:sup>13<\/jats:sup>\n                    C-labeled methane and methanol, followed by nanoscale secondary ion mass spectrometry, confirmed that carbon derived from both compounds was actively incorporated into the tissues within five days. Methano- and methylotrophs of the bacterial families Methylomonadaceae, Methylophagaceae and Methylophilaceae were abundant, based on environmental metagenomics and 16S rRNA sequencing, and fluorescence and electron microscopy confirmed dense epibiont aggregations on the sea spider exoskeleton. Egg sacs carried by the males hosted identical microbes suggesting vertical transmission. We propose that these sea spiders farm and feed on methanotrophic and methylotrophic bacteria, expanding the realm of animals known to harness C1 compounds as a carbon source. These findings advance our understanding of the biology of an understudied animal lineage, unlocking some of the unique nutritional links between the microbial and faunal food webs in the oceans.\n                  <\/jats:p>","DOI":"10.1073\/pnas.2501422122","type":"journal-article","created":{"date-parts":[[2025,6,16]],"date-time":"2025-06-16T15:50:29Z","timestamp":1750089029000},"update-policy":"https:\/\/doi.org\/10.1073\/pnas.cm10313","source":"Crossref","is-referenced-by-count":14,"title":["Methane-powered sea spiders: Diverse, epibiotic methanotrophs serve as a source of nutrition for deep-sea methane seep\n                    <i>Sericosura<\/i>"],"prefix":"10.1073","volume":"122","author":[{"ORCID":"https:\/\/orcid.org\/0009-0007-1691-8181","authenticated-orcid":false,"given":"Bianca","family":"Dal B\u00f3","sequence":"first","affiliation":[{"name":"Department of Biology, Occidental College","place":["Los Angeles"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0005-3983-8382","authenticated-orcid":false,"given":"Yongzhao","family":"Guo","sequence":"additional","affiliation":[{"name":"Division of Geological and Planetary Sciences, California Institute of Technology","place":["Pasadena"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Magdalena J.","family":"Mayr","sequence":"additional","affiliation":[{"name":"Division of Geological and Planetary Sciences, California Institute of Technology","place":["Pasadena"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Olivia S.","family":"Pereira","sequence":"additional","affiliation":[{"name":"Division of Integrated Oceanography, Scripps Institution of Oceanography, University of California San Diego","place":["San Diego"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2858-8622","authenticated-orcid":false,"given":"Lisa A.","family":"Levin","sequence":"additional","affiliation":[{"name":"Division of Integrated Oceanography, Scripps Institution of Oceanography, University of California San Diego","place":["San Diego"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5374-6178","authenticated-orcid":false,"given":"Victoria J.","family":"Orphan","sequence":"additional","affiliation":[{"name":"Division of Geological and Planetary Sciences, California Institute of Technology","place":["Pasadena"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9110-9591","authenticated-orcid":false,"given":"Shana K.","family":"Goffredi","sequence":"additional","affiliation":[{"name":"Department of Biology, Occidental College","place":["Los Angeles"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"341","published-online":{"date-parts":[[2025,6,16]]},"reference":[{"key":"e_1_3_4_1_2","doi-asserted-by":"publisher","DOI":"10.1038\/325346a0"},{"key":"e_1_3_4_2_2","first-page":"321","article-title":"New aspects of the symbiosis in the provannid snail Ifremeria nautilei from the North Fiji Back Arc Basin","volume":"43","author":"Borowski C.","year":"2002","unstructured":"C. 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