{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,22]],"date-time":"2026-09-22T09:59:54Z","timestamp":1790071194616,"version":"4.0.1"},"reference-count":66,"publisher":"National Academy of Sciences","issue":"28","license":[{"start":{"date-parts":[[2025,7,7]],"date-time":"2025-07-07T00:00:00Z","timestamp":1751846400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"SITplus, Hort Innovation","award":["FF17000"],"award-info":[{"award-number":["FF17000"]}]},{"DOI":"10.13039\/501100001659","name":"Deutsche Forschungsgemeinschaft","doi-asserted-by":"publisher","award":["SCHE 1833\/7-1"],"award-info":[{"award-number":["SCHE 1833\/7-1"]}],"id":[{"id":"10.13039\/501100001659","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Horizon Europe Research and Innovation Action","award":["101059523"],"award-info":[{"award-number":["101059523"]}]}],"content-domain":{"domain":["www.pnas.org"],"crossmark-restriction":true},"short-container-title":["Proc. Natl. Acad. Sci. U.S.A."],"published-print":{"date-parts":[[2025,7,15]]},"abstract":"<jats:p>\n                    The Sterile Insect Technique (SIT) is a species-specific and environmentally friendly method for effectively controlling pest insect populations based on releasing reared, sterile insects into infested areas. Sex sorting in rearing facilities, enabling male-only releases, is necessary to ensure SIT programs are efficient, cost-effective and, in case of mosquito control, also safe. This can be greatly facilitated by genetic sexing strains (GSS), exhibiting sex-specific phenotypic markers. However, the development of GSS remains challenging. The construction of a\n                    <jats:italic toggle=\"yes\">temperature-sensitive lethal<\/jats:italic>\n                    (\n                    <jats:italic toggle=\"yes\">tsl<\/jats:italic>\n                    )-based GSS in the Mediterranean fruit fly (\n                    <jats:italic toggle=\"yes\">Ceratitis capitata<\/jats:italic>\n                    ) over three decades ago was considered a major breakthrough for SIT programs but was never successfully replicated in other pests. After over 30 y of research, we have pinpointed a specific mutation in the\n                    <jats:italic toggle=\"yes\">C. capitata lysine--tRNA ligase<\/jats:italic>\n                    (\n                    <jats:italic toggle=\"yes\">Lysyl-tRNA synthetase<\/jats:italic>\n                    ,\n                    <jats:italic toggle=\"yes\">LysRS<\/jats:italic>\n                    ) gene responsible for the tsl phenotype. Introducing this specific mutation into a wild-type strain produced full embryonic lethality under heat stress, replicating the original mutant phenotype. The random integration of a\n                    <jats:italic toggle=\"yes\">LysRS<\/jats:italic>\n                    minigene reversed this effect. The high conservation of\n                    <jats:italic toggle=\"yes\">LysRS<\/jats:italic>\n                    among insects suggests that tsl-based GSS could be expanded to multiple pest species and extend applications of SIT programs for disease prevention and the protection of agriculture.\n                  <\/jats:p>","DOI":"10.1073\/pnas.2503604122","type":"journal-article","created":{"date-parts":[[2025,7,7]],"date-time":"2025-07-07T15:01:13Z","timestamp":1751900473000},"update-policy":"https:\/\/doi.org\/10.1073\/pnas.cm10313","source":"Crossref","is-referenced-by-count":9,"title":["Decoding and engineering temperature-sensitive lethality in\n                    <i>Ceratitis capitata<\/i>\n                    for pest control"],"prefix":"10.1073","volume":"122","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-5124-8477","authenticated-orcid":false,"given":"Roswitha A.","family":"Aumann","sequence":"first","affiliation":[{"name":"Department of Insect Biotechnology in Plant Protection, Institute for Insect Biotechnology, Justus-Liebig-University Gie\u00dfen","place":["Gie\u00dfen, Germany"]},{"name":"Liebig Centre for Agroecology and Climate Impact Research, International Atomic Energy Agency Collaborating Centre, Justus-Liebig-University Gie\u00dfen","place":["Gie\u00dfen, Germany"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Georgia","family":"Gouvi","sequence":"additional","affiliation":[{"name":"Insect Pest Control Section, Joint Food and Agriculture Organization of the United Nations\/International Atomic Energy Agency Centre of Nuclear Techniques in Food and Agriculture, Department of Nuclear Sciences and Applications, International Atomic Energy Agency","place":["Vienna, Austria"]},{"name":"Department of Sustainable Agriculture, Laboratory of Systems Microbiology and Applied Genomics, University of Patras","place":["Agrinio, Greece"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9979-2631","authenticated-orcid":false,"given":"Maria-Eleni","family":"Gregoriou","sequence":"additional","affiliation":[{"name":"Insect Pest Control Section, Joint Food and Agriculture Organization of the United Nations\/International Atomic Energy Agency Centre of Nuclear Techniques in Food and Agriculture, Department of Nuclear Sciences and Applications, International Atomic Energy Agency","place":["Vienna, Austria"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4173-9816","authenticated-orcid":false,"given":"Tanja","family":"Rehling","sequence":"additional","affiliation":[{"name":"Department of Insect Biotechnology in Plant Protection, Institute for Insect Biotechnology, Justus-Liebig-University Gie\u00dfen","place":["Gie\u00dfen, Germany"]},{"name":"Liebig Centre for Agroecology and Climate Impact Research, International Atomic Energy Agency Collaborating Centre, Justus-Liebig-University Gie\u00dfen","place":["Gie\u00dfen, Germany"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Germano","family":"Sollazzo","sequence":"additional","affiliation":[{"name":"Department of Insect Biotechnology in Plant Protection, Institute for Insect Biotechnology, Justus-Liebig-University Gie\u00dfen","place":["Gie\u00dfen, Germany"]},{"name":"Insect Pest Control Section, Joint Food and Agriculture Organization of the United Nations\/International Atomic Energy Agency Centre of Nuclear Techniques in Food and Agriculture, Department of Nuclear Sciences and Applications, International Atomic Energy Agency","place":["Vienna, Austria"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3999-4095","authenticated-orcid":false,"given":"Kostas","family":"Bourtzis","sequence":"additional","affiliation":[{"name":"Insect Pest Control Section, Joint Food and Agriculture 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McCombs, S. Saul, Translocation-based genetic sexing system for the Oriental fruit fly (Diptera: Tephritidae) based on pupal color dimorphism. Ann. Entomol. 88, 695\u2013698 (1995).","journal-title":"Ann. Entomol."},{"key":"e_1_3_4_10_2","doi-asserted-by":"publisher","DOI":"10.1186\/1471-2156-15-S2-S2"},{"key":"e_1_3_4_11_2","doi-asserted-by":"publisher","DOI":"10.1186\/s12863-020-00932-5"},{"key":"e_1_3_4_12_2","doi-asserted-by":"publisher","DOI":"10.1093\/jee\/toac166"},{"key":"e_1_3_4_13_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-020-20680-5"},{"key":"e_1_3_4_14_2","doi-asserted-by":"publisher","DOI":"10.1038\/s42003-025-07489-y"},{"key":"e_1_3_4_15_2","first-page":"367","volume-title":"Proceedings of 6th International Symposium on Fruit Flies of Economic Importance","author":"C\u00e1ceres C.","year":"2004","unstructured":"C. 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