{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,20]],"date-time":"2026-08-20T23:17:46Z","timestamp":1787267866663,"version":"build-2736575974"},"reference-count":50,"publisher":"National Academy of Sciences","issue":"33","license":[{"start":{"date-parts":[[2025,8,11]],"date-time":"2025-08-11T00:00:00Z","timestamp":1754870400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"content-domain":{"domain":["www.pnas.org"],"crossmark-restriction":true},"short-container-title":["Proc. Natl. Acad. Sci. U.S.A."],"published-print":{"date-parts":[[2025,8,19]]},"abstract":"<jats:p>\n                    The mechanically activated PIEZO1 ion channel is genetically linked to numerous physiological and pathophysiological processes. For example, deleting\n                    <jats:italic toggle=\"yes\">PIEZO1<\/jats:italic>\n                    in mice leads to defective lymphatic vessel development, while nonsense mutations in humans are associated with autosomal recessive generalized lymphatic dysplasia (GLD) and nonimmune hydrops fetalis. However, it remains unclear whether PIEZO1-dependent biological processes are directly mediated by its intrinsic mechanosensitivity. Here, we identified a human fetal hydrops\u2013associated single-residue mutation, L322P (corresponding to L329P in mouse PIEZO1). The mutant failed to show mechanically activated currents in response to poking or stretch of the cell membrane, but preserved normal plasma membrane expression and responsiveness to its chemical activators such as Yoda1 and Jedi1. Remarkably, the mechanical response of the mutant can be restored by Yoda1. These findings demonstrate a direct link between the loss of PIEZO1\u2019s mechanosensitivity and the pathophysiological phenotype of fetal hydrops and raise the therapeutic potential of using PIEZO1 chemical activators to restore the mechanosensitivity of PIEZO1 missense mutants that are associated with genetic diseases such as GLD and hydrops fetalis.\n                  <\/jats:p>","DOI":"10.1073\/pnas.2503793122","type":"journal-article","created":{"date-parts":[[2025,8,11]],"date-time":"2025-08-11T19:07:02Z","timestamp":1754939222000},"update-policy":"https:\/\/doi.org\/10.1073\/pnas.cm10313","source":"Crossref","is-referenced-by-count":7,"title":["The fetal hydrops\u2013associated single-residue mutation L322P disrupts mechanical but not chemical activation of the PIEZO1 ion channel"],"prefix":"10.1073","volume":"122","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-8154-7435","authenticated-orcid":false,"given":"Jinghui","family":"Jiang","sequence":"first","affiliation":[{"name":"State Key Laboratory of Membrane Biology, New Cornerstone Science Laboratory, Tsinghua-Peking Center for Life Sciences, Beijing Frontier Research Center of Biological Structure, IDG\/McGovern Institute for Brain Research, School of Pharmaceutical Sciences, Tsinghua University","place":["Beijing, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5586-2407","authenticated-orcid":false,"given":"Wei","family":"Guo","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Female Fertility Promotion, Center for Reproductive Medicine, Department of Obstetrics and Gynecology, Peking University Third Hospital","place":["Beijing, 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