{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,17]],"date-time":"2026-09-17T17:11:51Z","timestamp":1789665111224,"version":"4.0.1"},"reference-count":48,"publisher":"National Academy of Sciences","issue":"28","license":[{"start":{"date-parts":[[2025,7,8]],"date-time":"2025-07-08T00:00:00Z","timestamp":1751932800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"crossref","award":["82288102"],"award-info":[{"award-number":["82288102"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"crossref","award":["82222026"],"award-info":[{"award-number":["82222026"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"crossref","award":["82301886"],"award-info":[{"award-number":["82301886"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"crossref"}]},{"name":"National Key Research and Development Program of China","award":["2022YFC2702500"],"award-info":[{"award-number":["2022YFC2702500"]}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"crossref","award":["82271695"],"award-info":[{"award-number":["82271695"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":["www.pnas.org"],"crossmark-restriction":true},"short-container-title":["Proc. Natl. Acad. Sci. U.S.A."],"published-print":{"date-parts":[[2025,7,15]]},"abstract":"<jats:p>\n                    Progesterone receptor (PR)-mediated progesterone (P4) signaling plays a crucial role in the establishment of uterine receptivity which is the prerequisite for successful embryo implantation in mammals. However, detailed molecular mechanisms underlying PR\u2013chromatin binding and transcriptional activity in the uterus remain largely elusive. Here, combining the P4-administrated ovariectomized mouse model and PR\u2013chromatin immunoprecipitation sequencing, we identified transcription factor WT1 as a potential cooperator of PR in the uterus. WT1 was specifically expressed in uterine stromal cells. Uterine deletion of\n                    <jats:italic toggle=\"yes\">Wt1<\/jats:italic>\n                    resulted in implantation failure due to attenuated P4 responsiveness in stromal cells and aberrant uterine receptivity. Mechanistically, WT1 physically interacted with PR in stromal cells, and exhibited genome-wide co-occupancy with PR. Furthermore, WT1 was indispensable for directing PR onto the chromatin via corecruitment and tethering manners, and facilitated PR to activate the transcription of target genes that were critical for uterine receptivity. Collectively, our study provided substantial evidence that WT1 served as a functional partner of PR in uterine stromal cells, thus guaranteeing P4 responsiveness conducive to uterine receptivity.\n                  <\/jats:p>","DOI":"10.1073\/pnas.2504361122","type":"journal-article","created":{"date-parts":[[2025,7,8]],"date-time":"2025-07-08T11:39:52Z","timestamp":1751974792000},"update-policy":"https:\/\/doi.org\/10.1073\/pnas.cm10313","source":"Crossref","is-referenced-by-count":3,"title":["WT1 directs normal progesterone receptor\u2013chromatin binding essential for uterine receptivity at peri-implantation"],"prefix":"10.1073","volume":"122","author":[{"given":"Zhangli","family":"Ni","sequence":"first","affiliation":[{"name":"Fujian Provincial Key Laboratory of Reproductive Health Research, Department of Obstetrics and Gynecology, The First Affiliated Hospital of Xiamen University, School of Medicine, Xiamen University","place":["Xiamen, China"]},{"name":"State Key Laboratory of Farm Animal Biotech Breeding, College of Biological Sciences, China Agricultural University","place":["Beijing, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chuanhui","family":"Guo","sequence":"additional","affiliation":[{"name":"Fujian Provincial Key Laboratory of Reproductive Health Research, Department of Obstetrics and Gynecology, The First Affiliated Hospital of Xiamen University, School of Medicine, Xiamen University","place":["Xiamen, China"]},{"name":"State Key Laboratory of Farm Animal Biotech Breeding, College of Biological Sciences, China Agricultural University","place":["Beijing, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiaojing","family":"Liu","sequence":"additional","affiliation":[{"name":"Fujian Provincial Key Laboratory of Reproductive Health Research, Department of Obstetrics and Gynecology, The First Affiliated Hospital of Xiamen University, School of Medicine, Xiamen University","place":["Xiamen, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gaizhen","family":"Li","sequence":"additional","affiliation":[{"name":"Fujian Provincial Key Laboratory of Reproductive Health Research, Department of Obstetrics and Gynecology, The First Affiliated Hospital of Xiamen University, School of Medicine, Xiamen University","place":["Xiamen, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yedong","family":"Tang","sequence":"additional","affiliation":[{"name":"Fujian Provincial Key Laboratory of Reproductive Health Research, Department of Obstetrics and Gynecology, The First Affiliated Hospital of Xiamen University, School of Medicine, Xiamen University","place":["Xiamen, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Han","family":"Cai","sequence":"additional","affiliation":[{"name":"Fujian Provincial Key Laboratory of Reproductive Health Research, Department of Obstetrics and Gynecology, The First Affiliated Hospital of Xiamen University, School of Medicine, Xiamen University","place":["Xiamen, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Weiyu","family":"Huang","sequence":"additional","affiliation":[{"name":"Fujian Provincial Key Laboratory of Reproductive Health Research, Department of Obstetrics and Gynecology, The First Affiliated Hospital of Xiamen University, School of Medicine, Xiamen University","place":["Xiamen, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4029-6411","authenticated-orcid":false,"given":"Fei","family":"Gao","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Stem Cell and Reproductive Biology, Institute of Zoology, Chinese Academy of Sciences","place":["Beijing, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhaowei","family":"Tu","sequence":"additional","affiliation":[{"name":"Department of Obstetrics and Gynecology, The Third Affiliated Hospital of Guangzhou Medical University","place":["Guangzhou, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0415-503X","authenticated-orcid":false,"given":"Chao","family":"Wang","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Farm Animal Biotech Breeding, College of Biological Sciences, China Agricultural University","place":["Beijing, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9865-324X","authenticated-orcid":false,"given":"Haibin","family":"Wang","sequence":"additional","affiliation":[{"name":"Fujian Provincial Key Laboratory of Reproductive Health Research, Department of Obstetrics and Gynecology, The First Affiliated Hospital of Xiamen University, School of Medicine, Xiamen University","place":["Xiamen, China"]},{"name":"State Key Laboratory of Vaccines for Infectious Diseases, Xiang An Biomedicine Laboratory, School of Medicine, Xiamen University","place":["Xiamen, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7513-4041","authenticated-orcid":false,"given":"Shuangbo","family":"Kong","sequence":"additional","affiliation":[{"name":"Fujian Provincial Key Laboratory of Reproductive Health Research, Department of Obstetrics and Gynecology, The First Affiliated Hospital of Xiamen University, School of Medicine, Xiamen University","place":["Xiamen, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2101-3922","authenticated-orcid":false,"given":"Haili","family":"Bao","sequence":"additional","affiliation":[{"name":"Fujian Provincial Key Laboratory of Reproductive Health Research, Department of Obstetrics and Gynecology, The First Affiliated Hospital of Xiamen University, School of Medicine, Xiamen University","place":["Xiamen, 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R.","year":"1993","unstructured":"R. R. Rackley , Expression of the Wilms\u2019 tumor suppressor gene WT1 during mouse embryogenesis. Cell Growth Differ. 4, 1023\u20131031 (1993).","journal-title":"Cell Growth Differ."},{"key":"e_1_3_4_29_2","first-page":"5964","article-title":"Expression of Wilms\u2019 tumor suppressor gene (WT1) in human endometrium: Regulation through decidual differentiation","volume":"86","author":"Makrigiannakis A.","year":"2001","unstructured":"A. Makrigiannakis , Expression of Wilms\u2019 tumor suppressor gene (WT1) in human endometrium: Regulation through decidual differentiation. J. Clin. Endocrinol. Metab. 86, 5964\u20135972 (2001), 10.1210\/jcem.86.12.8074.","journal-title":"J. Clin. Endocrinol. 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Ni H. Bao PR ChIP-seq in OVX model WT1 ChIP-seq in the D4 uterus PR ChIP-seq in the D4 uterus PR ChIP-seq in Wt1f\/f and Wt1d\/d uteri on D4 RNA-seq in Wt1f\/f and Wt1d\/d uteri on D4. NCBI SRA. https:\/\/www.ncbi.nlm.nih.gov\/bioproject\/PRJNA1214385. Deposited 22 January 2025."},{"key":"e_1_3_4_48_2","unstructured":"Z. Ni H. Bao RNA-seq in OVX model. NCBI SRA. https:\/\/www.ncbi.nlm.nih.gov\/bioproject\/PRJNA1214981. 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