{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,23]],"date-time":"2026-10-23T21:43:29Z","timestamp":1792791809843,"version":"4.5.0"},"reference-count":44,"publisher":"National Academy of Sciences","issue":"41","license":[{"start":{"date-parts":[[2025,10,9]],"date-time":"2025-10-09T00:00:00Z","timestamp":1759968000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100003323","name":"Agence Nationale de Recherches sur le Sida et les H\u00e9patites Virales","doi-asserted-by":"publisher","award":["AO-2021-1 ECTZ162940"],"award-info":[{"award-number":["AO-2021-1 ECTZ162940"]}],"id":[{"id":"10.13039\/501100003323","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003323","name":"Agence Nationale de Recherches sur le Sida et les H\u00e9patites Virales","doi-asserted-by":"publisher","award":["AO-2021-1 ECTZ160109 and ANRS0644"],"award-info":[{"award-number":["AO-2021-1 ECTZ160109 and ANRS0644"]}],"id":[{"id":"10.13039\/501100003323","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100009060","name":"Sidaction","doi-asserted-by":"publisher","award":["AO2024"],"award-info":[{"award-number":["AO2024"]}],"id":[{"id":"10.13039\/100009060","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001665","name":"Agence Nationale de la Recherche","doi-asserted-by":"publisher","award":["ANR-24-CE15-2986"],"award-info":[{"award-number":["ANR-24-CE15-2986"]}],"id":[{"id":"10.13039\/501100001665","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100000781","name":"EC | European Research Council","doi-asserted-by":"publisher","award":["TubulinCode"],"award-info":[{"award-number":["TubulinCode"]}],"id":[{"id":"10.13039\/501100000781","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["www.pnas.org"],"crossmark-restriction":true},"short-container-title":["Proc. Natl. Acad. Sci. U.S.A."],"published-print":{"date-parts":[[2025,10,14]]},"abstract":"<jats:p>Like many viruses, HIV relies on the microtubule (MT) cytoskeleton for successful infection. MT-associated proteins (MAPs) regulate MT functions and thus bear the potential to modulate viral infection. However, while several MAPs are known to exert proviral effects on HIV, little is known about antiviral ones. We previously described the Tripartite motif protein 69 (Trim69) as an innate immune factor that remodels MTs, leading to inhibition of a broad spectrum of viruses, including HIV. Through in silico modeling, TIRF microscopy, and cell-based assays, we determine that Trim69 binding to MTs is determined by a basic surface in its SPRY domain that interacts with the C-terminal tails of tubulins. This surface is conserved among mammalian Trim69s and is critical for its functions. We demonstrate that by binding and remodeling MTs, Trim69 inhibits the docking and the migration of virion cores on MTs by promoting the stalling of the dynein\/dynactin motor complexes. Altogether, these findings shed light on a mechanism of viral defense that involves an innate immune regulation of the MT cytoskeleton.<\/jats:p>","DOI":"10.1073\/pnas.2505128122","type":"journal-article","created":{"date-parts":[[2025,10,9]],"date-time":"2025-10-09T15:31:13Z","timestamp":1760023873000},"update-policy":"https:\/\/doi.org\/10.1073\/pnas.cm10313","source":"Crossref","is-referenced-by-count":1,"title":["Microtubule remodeling by the innate immune factor Trim69 compromises dynein-dependent migration of HIV virion cores toward the nucleus"],"prefix":"10.1073","volume":"122","author":[{"given":"Charlotte","family":"Vadon","sequence":"first","affiliation":[{"name":"Centre International de Recherche en Infectiologie, Universit\u00e9 de Lyon, INSERM, U1111, Universit\u00e9 Claude Bernard Lyon 1, CNRS, UMR5308, \u00c9cole Nationale Sup\u00e9rieure de Lyon","place":["Lyon, France"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xuan-Nhi","family":"Nguyen","sequence":"additional","affiliation":[{"name":"Centre International de Recherche en Infectiologie, Universit\u00e9 de Lyon, INSERM, U1111, Universit\u00e9 Claude Bernard Lyon 1, CNRS, UMR5308, \u00c9cole Nationale Sup\u00e9rieure de Lyon","place":["Lyon, France"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Valerie","family":"Siahaan","sequence":"additional","affiliation":[{"name":"Institut Curie, Universit\u00e9 Paris Sciences et lettres, CNRS UMR3348","place":["Orsay, France"]},{"name":"Universit\u00e9 Paris-Saclay, CNRS UMR3348","place":["Orsay, France"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yuxin","family":"Song","sequence":"additional","affiliation":[{"name":"Centre International de Recherche en Infectiologie, Universit\u00e9 de Lyon, INSERM, U1111, Universit\u00e9 Claude Bernard Lyon 1, CNRS, UMR5308, \u00c9cole Nationale Sup\u00e9rieure de Lyon","place":["Lyon, France"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Arya","family":"Krishnan","sequence":"additional","affiliation":[{"name":"Institut Curie, Universit\u00e9 Paris Sciences et lettres, CNRS UMR3348","place":["Orsay, France"]},{"name":"Universit\u00e9 Paris-Saclay, CNRS UMR3348","place":["Orsay, France"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Veronique","family":"Henriot","sequence":"additional","affiliation":[{"name":"Institut Curie, Universit\u00e9 Paris Sciences et lettres, CNRS UMR3348","place":["Orsay, France"]},{"name":"Universit\u00e9 Paris-Saclay, CNRS UMR3348","place":["Orsay, France"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Amandine","family":"Chantharath","sequence":"additional","affiliation":[{"name":"Centre International de Recherche en Infectiologie, Universit\u00e9 de Lyon, INSERM, U1111, Universit\u00e9 Claude Bernard Lyon 1, CNRS, UMR5308, \u00c9cole Nationale Sup\u00e9rieure de Lyon","place":["Lyon, France"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Julien","family":"Burlaud-Gaillard","sequence":"additional","affiliation":[{"name":"Plateforme Infrastructures en Biologie Sante et Agronomie de Microscopie Electronique, Universit\u00e9 de Tours et Centre Hospitalier Universitaire de Tours","place":["Tours, France"]},{"name":"INSERM U1259, Universit\u00e9 de Tours et CHU de Tours","place":["Tours, France"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9131-3341","authenticated-orcid":false,"given":"Philippe","family":"Roingeard","sequence":"additional","affiliation":[{"name":"Plateforme Infrastructures en Biologie Sante et Agronomie de Microscopie Electronique, Universit\u00e9 de Tours et Centre Hospitalier Universitaire de Tours","place":["Tours, France"]},{"name":"INSERM U1259, Universit\u00e9 de Tours et CHU de Tours","place":["Tours, France"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7053-2000","authenticated-orcid":false,"given":"Carsten","family":"Janke","sequence":"additional","affiliation":[{"name":"Institut Curie, Universit\u00e9 Paris Sciences et lettres, CNRS UMR3348","place":["Orsay, France"]},{"name":"Universit\u00e9 Paris-Saclay, CNRS UMR3348","place":["Orsay, France"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8585-7534","authenticated-orcid":false,"given":"Lucie","family":"Etienne","sequence":"additional","affiliation":[{"name":"Centre International de Recherche en Infectiologie, Universit\u00e9 de Lyon, INSERM, U1111, Universit\u00e9 Claude Bernard Lyon 1, CNRS, UMR5308, \u00c9cole Nationale Sup\u00e9rieure de Lyon","place":["Lyon, France"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8388-1576","authenticated-orcid":false,"given":"Francesca","family":"Fiorini","sequence":"additional","affiliation":[{"name":"Molecular Microbiology and Structural Biochemistry, Microbiologie Mol\u00e9culaire et Biochimie Structurale-Institut de Biologie et de Chimie des Prot\u00e9ines, UMR 5086 CNRS University of Lyon","place":["Lyon, France"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maria M.","family":"Magiera","sequence":"additional","affiliation":[{"name":"Institut Curie, Universit\u00e9 Paris Sciences et lettres, CNRS UMR3348","place":["Orsay, France"]},{"name":"Universit\u00e9 Paris-Saclay, CNRS UMR3348","place":["Orsay, France"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0892-0488","authenticated-orcid":false,"given":"Andrea","family":"Cimarelli","sequence":"additional","affiliation":[{"name":"Centre International de Recherche en Infectiologie, Universit\u00e9 de Lyon, INSERM, U1111, Universit\u00e9 Claude Bernard Lyon 1, CNRS, UMR5308, \u00c9cole Nationale Sup\u00e9rieure de Lyon","place":["Lyon, France"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"341","published-online":{"date-parts":[[2025,10,9]]},"reference":[{"key":"e_1_3_4_1_2","doi-asserted-by":"publisher","DOI":"10.1146\/annurev-virology-020922-110929"},{"key":"e_1_3_4_2_2","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.200203150"},{"key":"e_1_3_4_3_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41418-020-0573-5"},{"key":"e_1_3_4_4_2","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.00358-18"},{"key":"e_1_3_4_5_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1712033114"},{"key":"e_1_3_4_6_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M114.613133"},{"key":"e_1_3_4_7_2","doi-asserted-by":"publisher","DOI":"10.1038\/ncomms7660"},{"key":"e_1_3_4_8_2","doi-asserted-by":"publisher","DOI":"10.1128\/jvi.00499-23"},{"key":"e_1_3_4_9_2","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.00819-08"},{"key":"e_1_3_4_10_2","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.00404-20"},{"key":"e_1_3_4_11_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.chom.2013.10.012"},{"key":"e_1_3_4_12_2","doi-asserted-by":"publisher","DOI":"10.15252\/embj.2020104870"},{"key":"e_1_3_4_13_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.ppat.1005700"},{"key":"e_1_3_4_14_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.tcb.2019.07.004"},{"key":"e_1_3_4_15_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.devcel.2020.01.029"},{"key":"e_1_3_4_16_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.cub.2021.02.035"},{"key":"e_1_3_4_17_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41580-020-0214-3"},{"key":"e_1_3_4_18_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.2211467119"},{"key":"e_1_3_4_19_2","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.01372-19"},{"key":"e_1_3_4_20_2","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.00951-19"},{"key":"e_1_3_4_21_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.ppat.1007287"},{"key":"e_1_3_4_22_2","doi-asserted-by":"publisher","DOI":"10.1128\/jvi.01026-24"},{"key":"e_1_3_4_23_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41586-021-03819-2"},{"key":"e_1_3_4_24_2","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gku316"},{"key":"e_1_3_4_25_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41586-024-07487-w"},{"key":"e_1_3_4_26_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.coviro.2012.02.003"},{"key":"e_1_3_4_27_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41556-021-00825-4"},{"key":"e_1_3_4_28_2","doi-asserted-by":"publisher","DOI":"10.1002\/cpz1.1070"},{"key":"e_1_3_4_29_2","doi-asserted-by":"publisher","DOI":"10.1021\/bi961356j"},{"key":"e_1_3_4_30_2","doi-asserted-by":"publisher","DOI":"10.1089\/aid.2014.0026"},{"key":"e_1_3_4_31_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.chom.2018.03.009"},{"key":"e_1_3_4_32_2","doi-asserted-by":"publisher","DOI":"10.1242\/jcs.259689"},{"key":"e_1_3_4_33_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41580-022-00491-w"},{"key":"e_1_3_4_34_2","doi-asserted-by":"publisher","DOI":"10.1107\/S2059798320010499"},{"key":"e_1_3_4_35_2","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.201902088"},{"key":"e_1_3_4_36_2","doi-asserted-by":"publisher","DOI":"10.1093\/hmg\/8.8.1387"},{"key":"e_1_3_4_37_2","doi-asserted-by":"publisher","DOI":"10.1006\/geno.1999.6043"},{"key":"e_1_3_4_38_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.96.6.2794"},{"key":"e_1_3_4_39_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.neuron.2015.11.012"},{"key":"e_1_3_4_40_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M512755200"},{"key":"e_1_3_4_41_2","doi-asserted-by":"publisher","DOI":"10.3390\/v16081328"},{"key":"e_1_3_4_42_2","doi-asserted-by":"publisher","DOI":"10.1093\/hmg\/8.8.1397"},{"key":"e_1_3_4_43_2","unstructured":"L. Etienne A. Chantharath TRIM69_mammals_prank.fasta. Figshare. https:\/\/doi.org\/10.6084\/m9.figshare.28330211.v1. Deposited 2 February 2025."},{"key":"e_1_3_4_44_2","unstructured":"L. Etienne A. Chantharath espript_trim69_mammals_prank.pdf. Figshare. https:\/\/figshare.com\/articles\/figure\/espript_trim69_mammals_prank_pdf\/28330208\/1?file=52091282. Deposited 2 February 2025."}],"container-title":["Proceedings of the National Academy of Sciences"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/pnas.org\/doi\/pdf\/10.1073\/pnas.2505128122","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,9]],"date-time":"2025-10-09T15:31:35Z","timestamp":1760023895000},"score":1,"resource":{"primary":{"URL":"https:\/\/pnas.org\/doi\/10.1073\/pnas.2505128122"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,10,9]]},"references-count":44,"journal-issue":{"issue":"41","published-print":{"date-parts":[[2025,10,14]]}},"alternative-id":["10.1073\/pnas.2505128122"],"URL":"https:\/\/doi.org\/10.1073\/pnas.2505128122","relation":{},"ISSN":["0027-8424","1091-6490"],"issn-type":[{"value":"0027-8424","type":"print"},{"value":"1091-6490","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025,10,9]]},"assertion":[{"value":"2025-03-06","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2025-09-03","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2025-10-09","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e2505128122"}}