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To clarify the underlying biology of\n                    <jats:italic toggle=\"yes\">JAG1<\/jats:italic>\n                    overexpression in dystrophic muscles, we generated a transgenic mouse (mdx\n                    <jats:sup>5cv<\/jats:sup>\n                    -\n                    <jats:italic toggle=\"yes\">JAG1<\/jats:italic>\n                    ) model that lacks dystrophin and overexpresses human\n                    <jats:italic toggle=\"yes\">JAG1<\/jats:italic>\n                    in striated muscles. Skeletal muscles from mdx\n                    <jats:sup>5cv<\/jats:sup>\n                    -\n                    <jats:italic toggle=\"yes\">JAG1<\/jats:italic>\n                    and mdx\n                    <jats:sup>5cv<\/jats:sup>\n                    mice were studied at 1-, 4-, and 12-mo time points.\n                    <jats:italic toggle=\"yes\">JAG1<\/jats:italic>\n                    expression in mdx\n                    <jats:sup>5cv<\/jats:sup>\n                    -\n                    <jats:italic toggle=\"yes\">JAG1<\/jats:italic>\n                    increased by 3 to 5 times compared to mdx\n                    <jats:sup>5cv<\/jats:sup>\n                    . Consequently, mdx\n                    <jats:sup>5cv<\/jats:sup>\n                    -\n                    <jats:italic toggle=\"yes\">JAG1<\/jats:italic>\n                    muscles were significantly bigger and stronger than dystrophic controls, along with an increased number of myofibers. Proteomics data show increased dysferlin in mdx\n                    <jats:sup>5cv<\/jats:sup>\n                    -\n                    <jats:italic toggle=\"yes\">JAG1<\/jats:italic>\n                    muscles and an association of the histone methyltransferase Nsd1 with the phenotype. Our data support the positive effect of\n                    <jats:italic toggle=\"yes\">JAG1<\/jats:italic>\n                    overexpression in dystrophic muscles.\n                  <\/jats:p>","DOI":"10.1073\/pnas.2506437122","type":"journal-article","created":{"date-parts":[[2025,9,23]],"date-time":"2025-09-23T15:49:17Z","timestamp":1758642557000},"update-policy":"https:\/\/doi.org\/10.1073\/pnas.cm10313","source":"Crossref","is-referenced-by-count":1,"title":["Muscle-specific increased expression of\n                    <i>JAG1<\/i>\n                    improves the skeletal muscle phenotype in dystrophin-deficient mice"],"prefix":"10.1073","volume":"122","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-2493-8872","authenticated-orcid":false,"given":"Felipe","family":"de Souza Leite","sequence":"first","affiliation":[{"name":"Division of Genetics and Genomics, Boston Children\u2019s 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