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Natl. Acad. Sci. U.S.A."],"published-print":{"date-parts":[[2025,9,9]]},"abstract":"<jats:p>\n                    Circadian clocks allow organisms to anticipate daily fluctuations in light and temperature, but how this anticipatory role promotes adaptation to different environments remains poorly understood. Here, we subjected the cyanobacterium\n                    <jats:italic toggle=\"yes\">Synechococcus elongatus<\/jats:italic>\n                    PCC 7942 to a long-term evolution experiment under high light, high temperature, and elevated CO\n                    <jats:sub>2<\/jats:sub>\n                    levels. After 1,200 generations, we obtained a strain exhibiting a 600% increase in growth rate. Whole-genome sequencing revealed three mutations fixed in the evolved population, two of which were sufficient to recapitulate the fast-growing phenotype in the wild type. A mutation in the promoter of the shikimate kinase\n                    <jats:italic toggle=\"yes\">aroK<\/jats:italic>\n                    led to its overexpression, while a mutation in the central circadian regulator\n                    <jats:italic toggle=\"yes\">sasA<\/jats:italic>\n                    disrupted both the phase and amplitude of the circadian rhythm. Changes in circadian control led to widespread perturbations in the transcriptome and metabolome. These included major shifts in the Calvin\u2013Benson\u2013Bassham cycle and glycogen storage dynamics. While these changes increased fitness under the experimental conditions, they caused maladaptation when light or CO\n                    <jats:sub>2<\/jats:sub>\n                    levels were altered, revealing a trade-off between fitness and environmental flexibility. Our results demonstrate that mutations in circadian control can drive fast adaptation by modulating central metabolism, underscoring the circadian cycle as a cornerstone of cellular plasticity. Thus, targeting the circadian cycle could be key to engineering cyanobacterial strains optimized for carbon fixation and biomass production.\n                  <\/jats:p>","DOI":"10.1073\/pnas.2506928122","type":"journal-article","created":{"date-parts":[[2025,9,3]],"date-time":"2025-09-03T17:42:56Z","timestamp":1756921376000},"update-policy":"https:\/\/doi.org\/10.1073\/pnas.cm10313","source":"Crossref","is-referenced-by-count":7,"title":["Mutations in the circadian cycle drive adaptive plasticity in cyanobacteria"],"prefix":"10.1073","volume":"122","author":[{"ORCID":"https:\/\/orcid.org\/0009-0002-0872-4882","authenticated-orcid":false,"given":"Alfonso","family":"Menda\u00f1a","sequence":"first","affiliation":[{"name":"Instituto de Biomedicina y Biotecnolog\u00eda de Cantabria, Universidad de Cantabria\u2014Consejo Superior de Investigaciones Cient\u00edficas","place":["Santander, Spain"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9624-2914","authenticated-orcid":false,"given":"Mar\u00eda","family":"Santos-Merino","sequence":"additional","affiliation":[{"name":"Instituto de Biomedicina y Biotecnolog\u00eda de Cantabria, Universidad de Cantabria\u2014Consejo Superior de Investigaciones Cient\u00edficas","place":["Santander, Spain"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0290-7020","authenticated-orcid":false,"given":"Raquel","family":"Guti\u00e9rrez-Lanza","sequence":"additional","affiliation":[{"name":"Instituto de Biomedicina y Biotecnolog\u00eda de Cantabria, Universidad de Cantabria\u2014Consejo Superior de Investigaciones Cient\u00edficas","place":["Santander, Spain"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marina","family":"Dom\u00ednguez-Quintero","sequence":"additional","affiliation":[{"name":"Instituto de Biomedicina y 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