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Natl. Acad. Sci. U.S.A."],"published-print":{"date-parts":[[2025,11,4]]},"abstract":"<jats:p>\n                    Pregnancy requires a supportive uterine environment facilitated by steroid hormone\u2013regulated differentiation of endometrial stromal fibroblasts into decidual cells and tight control of inflammation. Serum response factor (SRF) is a widely expressed transcription factor essential for mesenchymal cell growth and differentiation with noted roles in hormonal regulation of muscle tissues but little characterization in reproductive organs. Here, we reveal that endometrial SRF is dysregulated in human endometriosis and is critical for female reproductive success in mice through regulation of endometrial stromal and epithelial cells. Immunohistochemical analysis identified decreased endometrial SRF expression in infertile endometriosis patient tissues. RNAi-based\n                    <jats:italic toggle=\"yes\">SRF<\/jats:italic>\n                    knockdown in human endometrial stromal cells resulted in disrupted cytoskeletal structure, viability, and decidual response. Conditional\n                    <jats:italic toggle=\"yes\">Srf<\/jats:italic>\n                    knockout mice (Srf\n                    <jats:sup>d\/d<\/jats:sup>\n                    ) generated using the\n                    <jats:italic toggle=\"yes\">\n                      Pgr\n                      <jats:sup>Cre<\/jats:sup>\n                    <\/jats:italic>\n                    were infertile with implantation failure, lack of decidual response, and development of severe endometrial fibrosis. Single-cell RNA sequencing identified dramatic changes in Srf\n                    <jats:sup>d\/d<\/jats:sup>\n                    endometrial cell populations including large-scale myeloid immune cell infiltration. Srf\n                    <jats:sup>d\/d<\/jats:sup>\n                    stromal fibroblasts displayed aberrant cytoskeletal and extracellular matrix gene expression and downregulation of genes important for decidual growth response.\n                    <jats:italic toggle=\"yes\">Srf<\/jats:italic>\n                    -deficient epithelial cells displayed the most prominent dysregulation, strongly overexpressing estrogenic innate inflammatory genes including\n                    <jats:italic toggle=\"yes\">C3<\/jats:italic>\n                    and\n                    <jats:italic toggle=\"yes\">Lcn2<\/jats:italic>\n                    and fibrogenic genes\n                    <jats:italic toggle=\"yes\">Mmp7<\/jats:italic>\n                    and\n                    <jats:italic toggle=\"yes\">Fbln1<\/jats:italic>\n                    , all of which paralleled patterns in human endometriosis patient data that we identified through comparative analysis using a published single-cell atlas. These results demonstrate the profound impact of SRF on endometrial homeostasis with relevance to human endometriosis\u2013related infertility.\n                  <\/jats:p>","DOI":"10.1073\/pnas.2510060122","type":"journal-article","created":{"date-parts":[[2025,10,28]],"date-time":"2025-10-28T17:39:01Z","timestamp":1761673141000},"update-policy":"https:\/\/doi.org\/10.1073\/pnas.cm10313","source":"Crossref","is-referenced-by-count":4,"title":["Serum response factor is essential for endometrial function and prevention of inflammatory fibrosis"],"prefix":"10.1073","volume":"122","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-6597-4506","authenticated-orcid":false,"given":"Ryan M.","family":"Marquardt","sequence":"first","affiliation":[{"name":"Reproductive and Developmental Biology Laboratory, National Institute of Environmental Health Sciences","place":["Research Triangle 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