{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,5]],"date-time":"2026-10-05T21:58:17Z","timestamp":1791237497796,"version":"4.1.0"},"reference-count":72,"publisher":"National Academy of Sciences","issue":"49","license":[{"start":{"date-parts":[[2025,12,1]],"date-time":"2025-12-01T00:00:00Z","timestamp":1764547200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"funder":[{"DOI":"10.13039\/100000060","name":"HHS | NIH | National Institute of Allergy and Infectious Diseases","doi-asserted-by":"publisher","award":["AI178953"],"award-info":[{"award-number":["AI178953"]}],"id":[{"id":"10.13039\/100000060","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000060","name":"HHS | NIH | National Institute of Allergy and Infectious Diseases","doi-asserted-by":"publisher","award":["AI089474"],"award-info":[{"award-number":["AI089474"]}],"id":[{"id":"10.13039\/100000060","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000057","name":"HHS | NIH | National Institute of General Medical Sciences","doi-asserted-by":"publisher","award":["GM115234"],"award-info":[{"award-number":["GM115234"]}],"id":[{"id":"10.13039\/100000057","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["www.pnas.org"],"crossmark-restriction":true},"short-container-title":["Proc. Natl. Acad. Sci. U.S.A."],"published-print":{"date-parts":[[2025,12,9]]},"abstract":"<jats:p>IgG is a key to adaptive immunity and a critical platform for drug design. Sialic acid on the conserved glycan within the Fc domain is believed to promote anti-inflammatory IgG function; however, regulation of sialylation remains poorly defined. We previously showed that IgG sialylation is primarily mediated by B cell\u2013extrinsic processes in mice. Here, we found that IgG sialylation occurs in the subcellular compartments of the FcRn-mediated IgG recycling pathway of endothelial cells. This process is down-regulated by inflammatory signals and up-regulated during gestation, providing mechanistic insight into the epidemiology associating IgG glycosylation, pregnancy and inflammatory disease. These findings demonstrate that plasma-localized IgG glycosylation is dynamically altered by the endothelium, revealing a potential mechanism through which the function of all endogenous and administered IgG and Fc-containing pharmaceuticals could be altered.<\/jats:p>","DOI":"10.1073\/pnas.2511216122","type":"journal-article","created":{"date-parts":[[2025,12,1]],"date-time":"2025-12-01T20:00:36Z","timestamp":1764619236000},"update-policy":"https:\/\/doi.org\/10.1073\/pnas.cm10313","source":"Crossref","is-referenced-by-count":4,"title":["Endothelial cells sialylate IgG within the FcRn-mediated recycling pathway"],"prefix":"10.1073","volume":"122","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-0170-8783","authenticated-orcid":false,"given":"Leandre M.","family":"Glendenning","sequence":"first","affiliation":[{"name":"Department of Pathology, Case Western Reserve University","place":["Cleveland"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0005-3519-0814","authenticated-orcid":false,"given":"Megan D.","family":"Long","sequence":"additional","affiliation":[{"name":"Department of Pathology, Case Western Reserve University","place":["Cleveland"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0007-4065-5932","authenticated-orcid":false,"given":"Gracie C.","family":"Carlson","sequence":"additional","affiliation":[{"name":"Department of Pathology, Case Western Reserve University","place":["Cleveland"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Austin D.","family":"Silva","sequence":"additional","affiliation":[{"name":"Department of Cell, Developmental and Integrative Biology, University of Alabama at Birmingham","place":["Birmingham"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0007-5058-1447","authenticated-orcid":false,"given":"Siyu","family":"Wang","sequence":"additional","affiliation":[{"name":"Department of Pathology, Case Western Reserve University","place":["Cleveland"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kalob M.","family":"Reynero","sequence":"additional","affiliation":[{"name":"Department of Pathology, Case Western Reserve University","place":["Cleveland"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4892-0212","authenticated-orcid":false,"given":"Emily N.","family":"Kukan","sequence":"additional","affiliation":[{"name":"Department of Pathology, Case Western Reserve University","place":["Cleveland"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5071-527X","authenticated-orcid":false,"given":"Susan L.","family":"Bellis","sequence":"additional","affiliation":[{"name":"Department of Cell, Developmental and Integrative Biology, University of Alabama at Birmingham","place":["Birmingham"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6864-0384","authenticated-orcid":false,"given":"Wendy A.","family":"Goodman","sequence":"additional","affiliation":[{"name":"Department of Pathology, Case Western Reserve University","place":["Cleveland"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1055-2530","authenticated-orcid":false,"given":"Brian A.","family":"Cobb","sequence":"additional","affiliation":[{"name":"Department of Pathology, Case Western Reserve University","place":["Cleveland"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"341","published-online":{"date-parts":[[2025,12]]},"reference":[{"key":"e_1_3_4_1_2","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.134.6.4018"},{"key":"e_1_3_4_2_2","doi-asserted-by":"publisher","DOI":"10.1002\/eji.1830181113"},{"key":"e_1_3_4_3_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.1129594"},{"key":"e_1_3_4_4_2","doi-asserted-by":"publisher","DOI":"10.3389\/fimmu.2022.818736"},{"key":"e_1_3_4_5_2","doi-asserted-by":"publisher","DOI":"10.3390\/cells12172130"},{"key":"e_1_3_4_6_2","doi-asserted-by":"publisher","DOI":"10.1002\/eji.201344230"},{"key":"e_1_3_4_7_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41420-024-02180-3"},{"key":"e_1_3_4_8_2","doi-asserted-by":"publisher","DOI":"10.1038\/nri3401"},{"key":"e_1_3_4_9_2","doi-asserted-by":"publisher","DOI":"10.1586\/eci.10.9"},{"key":"e_1_3_4_10_2","doi-asserted-by":"publisher","DOI":"10.1111\/imr.13407"},{"key":"e_1_3_4_11_2","doi-asserted-by":"publisher","DOI":"10.1136\/annrheumdis-2011-201234.7"},{"key":"e_1_3_4_12_2","doi-asserted-by":"publisher","DOI":"10.1186\/ar2892"},{"key":"e_1_3_4_13_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.isci.2022.104088"},{"key":"e_1_3_4_14_2","doi-asserted-by":"publisher","DOI":"10.3389\/fimmu.2022.818382"},{"key":"e_1_3_4_15_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.coi.2022.102231"},{"key":"e_1_3_4_16_2","doi-asserted-by":"publisher","DOI":"10.1093\/glycob\/cwz065"},{"key":"e_1_3_4_17_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.ebiom.2022.103957"},{"key":"e_1_3_4_18_2","doi-asserted-by":"publisher","DOI":"10.3390\/biomedicines10081774"},{"key":"e_1_3_4_19_2","doi-asserted-by":"publisher","DOI":"10.3389\/fimmu.2022.790895"},{"key":"e_1_3_4_20_2","doi-asserted-by":"publisher","DOI":"10.1007\/s11481-021-09996-1"},{"key":"e_1_3_4_21_2","doi-asserted-by":"publisher","DOI":"10.3934\/genet.2016.4.280"},{"key":"e_1_3_4_22_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.ebiom.2022.104101"},{"key":"e_1_3_4_23_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-021-85200-x"},{"key":"e_1_3_4_24_2","doi-asserted-by":"publisher","DOI":"10.3390\/ijms23158473"},{"key":"e_1_3_4_25_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1523968113"},{"key":"e_1_3_4_26_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M114.595058"},{"key":"e_1_3_4_27_2","doi-asserted-by":"publisher","DOI":"10.1002\/JLB.3MA0522-731R"},{"key":"e_1_3_4_28_2","doi-asserted-by":"publisher","DOI":"10.1093\/glycob\/cwac039"},{"key":"e_1_3_4_29_2","doi-asserted-by":"publisher","DOI":"10.1093\/glycob\/cwad052"},{"key":"e_1_3_4_30_2","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.179.7.4580"},{"key":"e_1_3_4_31_2","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.1403014"},{"key":"e_1_3_4_32_2","doi-asserted-by":"publisher","DOI":"10.3389\/fimmu.2019.01540"},{"key":"e_1_3_4_33_2","doi-asserted-by":"publisher","DOI":"10.1093\/intimm\/dxg018"},{"key":"e_1_3_4_34_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.93.11.5512"},{"key":"e_1_3_4_35_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.sbi.2010.01.010"},{"key":"e_1_3_4_36_2","doi-asserted-by":"publisher","DOI":"10.1093\/intimm\/10.9.1289"},{"key":"e_1_3_4_37_2","doi-asserted-by":"publisher","DOI":"10.1080\/19420862.2019.1602459"},{"key":"e_1_3_4_38_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.cellimm.2018.07.009"},{"key":"e_1_3_4_39_2","doi-asserted-by":"publisher","DOI":"10.4161\/mabs.27765"},{"key":"e_1_3_4_40_2","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.1800896"},{"key":"e_1_3_4_41_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.immuni.2022.11.014"},{"key":"e_1_3_4_42_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M117.794248"},{"key":"e_1_3_4_43_2","doi-asserted-by":"publisher","DOI":"10.3390\/ijms22063048"},{"key":"e_1_3_4_44_2","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2006-05-024539"},{"key":"e_1_3_4_45_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.RA119.009539"},{"key":"e_1_3_4_46_2","doi-asserted-by":"publisher","DOI":"10.1158\/1078-0432.CCR-17-3533"},{"key":"e_1_3_4_47_2","doi-asserted-by":"publisher","DOI":"10.1093\/glycob\/cwr028"},{"key":"e_1_3_4_48_2","doi-asserted-by":"publisher","DOI":"10.1093\/glycob\/4.6.917"},{"key":"e_1_3_4_49_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jbc.2021.100354"},{"key":"e_1_3_4_50_2","doi-asserted-by":"publisher","DOI":"10.15252\/embj.201488856"},{"key":"e_1_3_4_51_2","doi-asserted-by":"publisher","DOI":"10.1002\/iid3.22"},{"key":"e_1_3_4_52_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-018-23481-5"},{"key":"e_1_3_4_53_2","doi-asserted-by":"publisher","DOI":"10.1186\/s13048-019-0574-5"},{"key":"e_1_3_4_54_2","doi-asserted-by":"publisher","DOI":"10.1152\/physiolgenomics.00125.2015"},{"key":"e_1_3_4_55_2","doi-asserted-by":"publisher","DOI":"10.3389\/fimmu.2017.01294"},{"key":"e_1_3_4_56_2","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.1502136"},{"key":"e_1_3_4_57_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jim.2012.05.022"},{"key":"e_1_3_4_58_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jim.2007.07.014"},{"key":"e_1_3_4_59_2","doi-asserted-by":"publisher","DOI":"10.1074\/mcp.M114.039537"},{"key":"e_1_3_4_60_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jaci.2022.10.035"},{"key":"e_1_3_4_61_2","doi-asserted-by":"publisher","DOI":"10.1002\/j.1460-2075.1991.tb07819.x"},{"key":"e_1_3_4_62_2","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.162.4.2162"},{"key":"e_1_3_4_63_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.ppat.1005456"},{"key":"e_1_3_4_64_2","doi-asserted-by":"publisher","DOI":"10.3389\/fimmu.2019.03024"},{"key":"e_1_3_4_65_2","doi-asserted-by":"publisher","DOI":"10.3389\/fimmu.2024.1402000"},{"key":"e_1_3_4_66_2","doi-asserted-by":"publisher","DOI":"10.1093\/glycob\/cwac056"},{"key":"e_1_3_4_67_2","doi-asserted-by":"publisher","DOI":"10.1038\/nature04478"},{"key":"e_1_3_4_68_2","doi-asserted-by":"publisher","DOI":"10.1097\/01.moh.0000219664.26528.da"},{"key":"e_1_3_4_69_2","doi-asserted-by":"publisher","DOI":"10.1093\/glycob\/cwae023"},{"key":"e_1_3_4_70_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41586-022-04816-9"},{"key":"e_1_3_4_71_2","doi-asserted-by":"publisher","DOI":"10.3389\/fimmu.2018.03166"},{"key":"e_1_3_4_72_2","doi-asserted-by":"publisher","DOI":"10.1021\/acs.jproteome.6b00038"}],"container-title":["Proceedings of the National Academy of Sciences"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/pnas.org\/doi\/pdf\/10.1073\/pnas.2511216122","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,12,1]],"date-time":"2025-12-01T20:00:47Z","timestamp":1764619247000},"score":1,"resource":{"primary":{"URL":"https:\/\/pnas.org\/doi\/10.1073\/pnas.2511216122"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,12]]},"references-count":72,"journal-issue":{"issue":"49","published-print":{"date-parts":[[2025,12,9]]}},"alternative-id":["10.1073\/pnas.2511216122"],"URL":"https:\/\/doi.org\/10.1073\/pnas.2511216122","relation":{},"ISSN":["0027-8424","1091-6490"],"issn-type":[{"value":"0027-8424","type":"print"},{"value":"1091-6490","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025,12]]},"assertion":[{"value":"2025-05-05","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2025-10-08","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2025-12-01","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e2511216122"}}