{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,4]],"date-time":"2026-09-04T17:59:40Z","timestamp":1788544780473,"version":"build-2803163510"},"reference-count":53,"publisher":"National Academy of Sciences","issue":"49","license":[{"start":{"date-parts":[[2025,12,4]],"date-time":"2025-12-04T00:00:00Z","timestamp":1764806400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100000038","name":"Natural Sciences and Engineering Research Council of Canada","doi-asserted-by":"publisher","award":["RGPIN-2023-05135"],"award-info":[{"award-number":["RGPIN-2023-05135"]}],"id":[{"id":"10.13039\/501100000038","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100000854","name":"Human Frontier Science Program","doi-asserted-by":"publisher","award":["RGP0054\/2020"],"award-info":[{"award-number":["RGP0054\/2020"]}],"id":[{"id":"10.13039\/501100000854","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001691","name":"MEXT | Japan Society for the Promotion of Science","doi-asserted-by":"publisher","award":["22J20318"],"award-info":[{"award-number":["22J20318"]}],"id":[{"id":"10.13039\/501100001691","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100000854","name":"Human Frontier Science Program","doi-asserted-by":"publisher","award":["LT0048\/2023-L"],"award-info":[{"award-number":["LT0048\/2023-L"]}],"id":[{"id":"10.13039\/501100000854","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["www.pnas.org"],"crossmark-restriction":true},"short-container-title":["Proc. Natl. Acad. Sci. U.S.A."],"published-print":{"date-parts":[[2025,12,9]]},"abstract":"<jats:p>Phosphate is often a limiting resource, directly affecting the availability of key biomolecules such as nucleotides. To cope with phosphate scarcity, bacteria have evolved enzymes that utilize alternative phosphorus compounds, including phosphite (Pt). Although a few enzymes oxidize Pt to produce phosphate, the enzymes responsible for Pt oxidation in many environmental bacteria remain unidentified, and the role of microbial Pt oxidation in the global phosphorus cycle is not yet fully understood. In this study, we performed bioinformatic analyses of three Pt-oxidizing enzymes: the native Pt oxidase, phosphite dehydrogenase (PtxD), and two promiscuous Pt oxidases, alkaline phosphatase (PhoA) and carbon\u2013phosphorus lyase. Among these, PhoA was found to be widely distributed across bacteria since the early stages of their evolution. In contrast, PtxD emerged later in a limited number of bacterial lineages that had lost PhoA. Our biochemical characterizations revealed that most extant and reconstructed ancestral PhoAs tested exhibited Pt oxidation activity. Moreover, disruption of active-site residues diminished Pt oxidase activity in PhoA, while only partially affecting its native function. This promiscuous function of PhoA reveals an overlooked mechanism in bacterial phosphate metabolism and underscores the role of Pt in the cycling of bioavailable phosphorus in ecosystems.<\/jats:p>","DOI":"10.1073\/pnas.2513042122","type":"journal-article","created":{"date-parts":[[2025,12,4]],"date-time":"2025-12-04T19:11:27Z","timestamp":1764875487000},"update-policy":"https:\/\/doi.org\/10.1073\/pnas.cm10313","source":"Crossref","is-referenced-by-count":3,"title":["Widespread promiscuous alkaline phosphatases underscore ancient microbial phosphite utilization"],"prefix":"10.1073","volume":"122","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-8804-9831","authenticated-orcid":false,"given":"Morito","family":"Sakuma","sequence":"first","affiliation":[{"name":"Michael Smith Laboratories, Faculty of Science, University of British Columbia","place":["Vancouver, Canada"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Naoki","family":"Konno","sequence":"additional","affiliation":[{"name":"Department of Biological Sciences, Graduate School of Science, The University of Tokyo","place":["Tokyo, Japan"]},{"name":"Department of Biological Sciences, Graduate School of Science, Universal Biology Institute, The University of Tokyo","place":["Tokyo, Japan"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sevan","family":"Gholipour","sequence":"additional","affiliation":[{"name":"Michael Smith Laboratories, Faculty of Science, University of British Columbia","place":["Vancouver, Canada"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"John Z.","family":"Chen","sequence":"additional","affiliation":[{"name":"Research School of Chemistry, College of Science, Australian National University","place":["Canberra, Australia"]},{"name":"Australian Research Council Centre of Excellence in Synthetic Biology, Research School of Biology, College of Science, Australian National University","place":["Canberra, Australia"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8235-1829","authenticated-orcid":false,"given":"Nobuhiko","family":"Tokuriki","sequence":"additional","affiliation":[{"name":"Michael Smith Laboratories, Faculty of Science, University of British Columbia","place":["Vancouver, Canada"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"341","published-online":{"date-parts":[[2025,12,4]]},"reference":[{"key":"e_1_3_4_1_2","doi-asserted-by":"publisher","DOI":"10.1016\/bs.aambs.2016.09.004"},{"key":"e_1_3_4_2_2","doi-asserted-by":"publisher","DOI":"10.3389\/fmicb.2023.1239189"},{"key":"e_1_3_4_3_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41579-024-01119-w"},{"key":"e_1_3_4_4_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-017-01418-8"},{"key":"e_1_3_4_5_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41396-021-01013-w"},{"key":"e_1_3_4_6_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.2118122119"},{"key":"e_1_3_4_7_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.2203604119"},{"key":"e_1_3_4_8_2","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.micro.61.080706.093357"},{"key":"e_1_3_4_9_2","doi-asserted-by":"publisher","DOI":"10.1111\/1462-2920.14628"},{"key":"e_1_3_4_10_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.tim.2018.01.002"},{"key":"e_1_3_4_11_2","doi-asserted-by":"publisher","DOI":"10.1098\/rstb.2006.1901"},{"key":"e_1_3_4_12_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0708205105"},{"key":"e_1_3_4_13_2","doi-asserted-by":"publisher","DOI":"10.1038\/ngeo580"},{"key":"e_1_3_4_14_2","doi-asserted-by":"publisher","DOI":"10.1021\/es901469t"},{"key":"e_1_3_4_15_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M011764200"},{"key":"e_1_3_4_16_2","doi-asserted-by":"publisher","DOI":"10.1021\/bi2016926"},{"key":"e_1_3_4_17_2","doi-asserted-by":"publisher","DOI":"10.1128\/JB.180.21.5547-5558.1998"},{"key":"e_1_3_4_18_2","doi-asserted-by":"publisher","DOI":"10.1186\/1471-2164-14-753"},{"key":"e_1_3_4_19_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1715549114"},{"key":"e_1_3_4_20_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.2020024118"},{"key":"e_1_3_4_21_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-024-47914-0"},{"key":"e_1_3_4_22_2","doi-asserted-by":"publisher","DOI":"10.1007\/s00203-011-0775-9"},{"key":"e_1_3_4_23_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-017-01226-8"},{"key":"e_1_3_4_24_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-019-46557-2"},{"key":"e_1_3_4_25_2","doi-asserted-by":"crossref","unstructured":"S. Gholipour J. Chen D. Lee N. Tokuriki The global \u03b2-lactam resistome revealed by comprehensive sequence analysis. bioRxiv [Preprint] (2024). https:\/\/doi.org\/10.1101\/2024.03.01.583042 (Accessed 4 March 2024).","DOI":"10.1101\/2024.03.01.583042"},{"key":"e_1_3_4_26_2","doi-asserted-by":"publisher","DOI":"10.1128\/jb.173.2.587-600.1991"},{"key":"e_1_3_4_27_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0400664101"},{"key":"e_1_3_4_28_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbapap.2012.07.015"},{"key":"e_1_3_4_29_2","doi-asserted-by":"publisher","DOI":"10.1021\/jacs.6b06186"},{"key":"e_1_3_4_30_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M117.788240"},{"key":"e_1_3_4_31_2","doi-asserted-by":"publisher","DOI":"10.1021\/jacs.2c06693"},{"key":"e_1_3_4_32_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.1254237"},{"key":"e_1_3_4_33_2","doi-asserted-by":"publisher","DOI":"10.1038\/ismej.2009.10"},{"key":"e_1_3_4_34_2","doi-asserted-by":"publisher","DOI":"10.1128\/JB.186.14.4730-4739.2004"},{"key":"e_1_3_4_35_2","doi-asserted-by":"publisher","DOI":"10.1126\/sciadv.adc9130"},{"key":"e_1_3_4_36_2","doi-asserted-by":"publisher","DOI":"10.1006\/jtbi.1997.0524"},{"key":"e_1_3_4_37_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41559-024-02461-1"},{"key":"e_1_3_4_38_2","doi-asserted-by":"publisher","DOI":"10.1093\/sysbio\/syac052"},{"key":"e_1_3_4_39_2","doi-asserted-by":"publisher","DOI":"10.1093\/molbev\/msz131"},{"key":"e_1_3_4_40_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbapap.2008.09.020"},{"key":"e_1_3_4_41_2","doi-asserted-by":"publisher","DOI":"10.1016\/0022-2836(91)90724-K"},{"key":"e_1_3_4_42_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pcbi.1010633"},{"key":"e_1_3_4_43_2","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.67.10.4504-4511.2001"},{"key":"e_1_3_4_44_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jmb.2010.04.057"},{"key":"e_1_3_4_45_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0014-5793(99)01448-9"},{"key":"e_1_3_4_46_2","doi-asserted-by":"publisher","DOI":"10.1038\/s42003-021-01918-4"},{"key":"e_1_3_4_47_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M605449200"},{"key":"e_1_3_4_48_2","volume-title":"Origins of Life: The Primal Self-Organization","author":"Pasek M. A.","year":"2011","unstructured":"M. A. Pasek, T. P. Kee, Origins of Life: The Primal Self-Organization (Springer, 2011)."},{"key":"e_1_3_4_49_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1408134111"},{"key":"e_1_3_4_50_2","doi-asserted-by":"publisher","DOI":"10.1039\/p29910000263"},{"key":"e_1_3_4_51_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.aaa8181"},{"key":"e_1_3_4_52_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.cbpa.2018.09.018"},{"key":"e_1_3_4_53_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.scitotenv.2023.163463"}],"container-title":["Proceedings of the National Academy of Sciences"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/pnas.org\/doi\/pdf\/10.1073\/pnas.2513042122","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,12,4]],"date-time":"2025-12-04T19:12:08Z","timestamp":1764875528000},"score":1,"resource":{"primary":{"URL":"https:\/\/pnas.org\/doi\/10.1073\/pnas.2513042122"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,12,4]]},"references-count":53,"journal-issue":{"issue":"49","published-print":{"date-parts":[[2025,12,9]]}},"alternative-id":["10.1073\/pnas.2513042122"],"URL":"https:\/\/doi.org\/10.1073\/pnas.2513042122","relation":{},"ISSN":["0027-8424","1091-6490"],"issn-type":[{"value":"0027-8424","type":"print"},{"value":"1091-6490","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025,12,4]]},"assertion":[{"value":"2025-05-21","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2025-10-10","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2025-12-04","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e2513042122"}}