{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,21]],"date-time":"2026-08-21T13:40:55Z","timestamp":1787319655876,"version":"build-2736575974"},"reference-count":81,"publisher":"National Academy of Sciences","issue":"4","license":[{"start":{"date-parts":[[2026,1,23]],"date-time":"2026-01-23T00:00:00Z","timestamp":1769126400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"funder":[{"DOI":"10.13039\/100000057","name":"HHS | NIH | National Institute of General Medical Sciences","doi-asserted-by":"publisher","award":["1FI2GM150430-01"],"award-info":[{"award-number":["1FI2GM150430-01"]}],"id":[{"id":"10.13039\/100000057","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000057","name":"HHS | NIH | National Institute of General Medical Sciences","doi-asserted-by":"publisher","award":["1FI2GM137843-01"],"award-info":[{"award-number":["1FI2GM137843-01"]}],"id":[{"id":"10.13039\/100000057","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["www.pnas.org"],"crossmark-restriction":true},"short-container-title":["Proc. Natl. Acad. Sci. U.S.A."],"published-print":{"date-parts":[[2026,1,27]]},"abstract":"<jats:p>\n                    Microorganisms commonly exist in polymicrobial communities, where they can respond to interspecies secreted molecules by altering behaviors and physiology; however, the underlying mechanisms remain underexplored. Here, we investigated interactions between\n                    <jats:italic toggle=\"yes\">Stenotrophomonas maltophilia<\/jats:italic>\n                    and\n                    <jats:italic toggle=\"yes\">\n                      <jats:italic toggle=\"yes\">Pseudomonas aeruginosa<\/jats:italic>\n                    <\/jats:italic>\n                    , coinfecting opportunistic pathogens found in pneumonia and chronic lung infections, such as in cystic fibrosis. We found that\n                    <jats:italic toggle=\"yes\">S. maltophilia<\/jats:italic>\n                    forms large protective multicellular aggregates upon exposure to\n                    <jats:italic toggle=\"yes\">\n                      <jats:italic toggle=\"yes\">P. aeruginosa<\/jats:italic>\n                    <\/jats:italic>\n                    secreted factors. Experimental evolution for lack of aggregation selected for fimbrial mutations and we found that fimbriae are required on both interacting\n                    <jats:italic toggle=\"yes\">S. maltophilia<\/jats:italic>\n                    cells for aggregation. Untargeted metabolomics and targeted validations revealed that the quorum-sensing molecule\n                    <jats:italic toggle=\"yes\">\n                      <jats:italic toggle=\"yes\">Pseudomonas<\/jats:italic>\n                    <\/jats:italic>\n                    quinolone signal (PQS) directly induced\n                    <jats:italic toggle=\"yes\">S. maltophilia<\/jats:italic>\n                    aggregation, and colocalized with the aggregates. Further, in coculture with\n                    <jats:italic toggle=\"yes\">\n                      <jats:italic toggle=\"yes\">P. aeruginosa<\/jats:italic>\n                    <\/jats:italic>\n                    , wild-type\n                    <jats:italic toggle=\"yes\">S. maltophilia<\/jats:italic>\n                    formed aggregates, resulting in up to 75-fold increased survival from\n                    <jats:italic toggle=\"yes\">\n                      <jats:italic toggle=\"yes\">P. aeruginosa<\/jats:italic>\n                    <\/jats:italic>\n                    competition compared to fimbrial mutants. Finally, multiple other bacterial species similarly aggregated upon exposure to\n                    <jats:italic toggle=\"yes\">\n                      <jats:italic toggle=\"yes\">P. aeruginosa<\/jats:italic>\n                    <\/jats:italic>\n                    PQS, indicating a more general response. Collectively, our work identifies a multispecies interaction where a quorum-sensing molecule from a coinfecting pathogen is sensed as a \u201cdanger\u201d signal, thereby inducing a protective multicellular response.\n                  <\/jats:p>","DOI":"10.1073\/pnas.2513122123","type":"journal-article","created":{"date-parts":[[2026,1,23]],"date-time":"2026-01-23T18:50:12Z","timestamp":1769194212000},"update-policy":"https:\/\/doi.org\/10.1073\/pnas.cm10313","source":"Crossref","is-referenced-by-count":3,"title":["A quorum-sensing molecule from\n                    <i>Pseudomonas aeruginosa<\/i>\n                    induces defensive multicellularity in a coinfecting pathogen"],"prefix":"10.1073","volume":"123","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-8710-2058","authenticated-orcid":false,"given":"Stefan","family":"Katharios-Lanwermeyer","sequence":"first","affiliation":[{"name":"Laboratory of Molecular Biology, Center for Cancer Research, National Cancer Institute, 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Health","place":["Bethesda"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0000-2680-0020","authenticated-orcid":false,"given":"Stefanie","family":"Severin","sequence":"additional","affiliation":[{"name":"Laboratory of Molecular Biology, Center for Cancer Research, National Cancer Institute, National Institutes of Health","place":["Bethesda"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alejandro E.","family":"Casiano","sequence":"additional","affiliation":[{"name":"Laboratory of Molecular Biology, Center for Cancer Research, National Cancer Institute, National Institutes of Health","place":["Bethesda"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chin-Hsien","family":"Tai","sequence":"additional","affiliation":[{"name":"Laboratory of Molecular Biology, Center for Cancer Research, National Cancer Institute, National Institutes of 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