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Natl. Acad. Sci. U.S.A."],"published-print":{"date-parts":[[2025,11,18]]},"abstract":"<jats:p>Epigenetic and transcriptional dysregulation plays a fundamental role in tumor lineage plasticity (LP). However, the underlying mechanisms, especially for the initial events of LP development, are still poorly understood. Here, we report that in progression of prostate cancer from adenocarcinoma to treatment-induced neuroendocrine prostate cancer (t-NEPC), anti-androgen receptor (AR) signaling inhibitors (ARSIs) reprogram the function of circadian regulator\/nuclear receptor REV-ERB\u03b1 by switching its target gene programs from kinase signaling and metabolic programs to programs of LP, which includes neurogenesis, stem cell, and epithelial\u2013mesenchymal transition as well as over fifteen LP drivers including POU3F2\/BRN2, ASCL1, FOXA2, ONECUT2, and MYCN. Unexpectedly, REV-ERB\u03b1 facilitates the chromatin occupancy of BRN2, ASCL1, and FOXA1 in their activation of LP programs, thus functioning as a master regulator of ARSI-induced LP driver network. Mechanistically, REV-ERB\u03b1 induces chromatin accessibility and H3K27ac modification at promoters of LP genes through its recruitment of BRD4 and p300. Overexpression of REV-ERB\u03b1 alone is sufficient to induce LP and neuroendocrine phenotype and confers resistance to ARSI in adenocarcinoma cells. Loss of REV-ERB\u03b1 potently inhibits NEPC cell growth and abolishes the expression of LP drivers and gene programs. Pharmacological inhibition of REV-ERB\u03b1 exhibits high potency in blocking the growth of NEPC tumors including patient-derived xenografts. Our findings reveal that therapy-induced LP development entails a coordinated induction of a network of LP drivers and that REV-ERB\u03b1 is an unexpected master regulator of the network and a promising therapeutic target for treatment of advanced prostate cancer such as NEPC.<\/jats:p>","DOI":"10.1073\/pnas.2513468122","type":"journal-article","created":{"date-parts":[[2025,11,13]],"date-time":"2025-11-13T18:52:58Z","timestamp":1763059978000},"update-policy":"https:\/\/doi.org\/10.1073\/pnas.cm10313","source":"Crossref","is-referenced-by-count":4,"title":["Circadian regulator REV-ERB\u03b1 is a master regulator of tumor lineage plasticity and an effective therapeutic target"],"prefix":"10.1073","volume":"122","author":[{"given":"Xiong","family":"Zhang","sequence":"first","affiliation":[{"name":"Department of Biochemistry and Molecular Medicine, School of Medicine, University of California 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