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The vertebrate CarGAP (i.e., Cx-CarGAP), created by genetically fusing connexins with CarH\n                    <jats:sub>C<\/jats:sub>\n                    in mammalian cells, can efficiently block the gap junction channels through AdoB\n                    <jats:sub>12<\/jats:sub>\n                    -induced protein oligomerization and subsequently reinstate them via green light\u2013induced protein disassembly. We further introduced the CarGAP system (i.e., Inx-CarGAP) to the\n                    <jats:italic toggle=\"yes\">Drosophila<\/jats:italic>\n                    ovary, enabling reversible control over the heterotypic gap junctions formed by innexin2 (Inx2) and innexin4 (Inx4, also known as zero population growth, Zpg), thereby uncovering the roles of gap junctions in stem cell\u2013niche interactions. This study illustrates CarGAP as a generalizable chemo-optogenetic tool for interrogating the functions of gap junctions in various biological contexts.\n                  <\/jats:p>","DOI":"10.1073\/pnas.2518037122","type":"journal-article","created":{"date-parts":[[2025,12,23]],"date-time":"2025-12-23T16:48:15Z","timestamp":1766508495000},"update-policy":"https:\/\/doi.org\/10.1073\/pnas.cm10313","source":"Crossref","is-referenced-by-count":4,"title":["Controllable gap junctions by vitamin B\n                    <sub>12<\/sub>\n                    and light"],"prefix":"10.1073","volume":"122","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-5158-6086","authenticated-orcid":false,"given":"Duo","family":"Cui","sequence":"first","affiliation":[{"name":"Department of Chemical and Biological Engineering, The Hong Kong University of Science and Technology","place":["Kowloon, Hong Kong SAR, 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