{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,5]],"date-time":"2026-09-05T14:30:57Z","timestamp":1788618657039,"version":"build-2803163510"},"reference-count":55,"publisher":"National Academy of Sciences","issue":"9","license":[{"start":{"date-parts":[[2026,2,23]],"date-time":"2026-02-23T00:00:00Z","timestamp":1771804800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"funder":[{"DOI":"10.13039\/100009633","name":"HHS | NIH | Eunice Kennedy Shriver National Institute of Child Health and Human Development","doi-asserted-by":"publisher","award":["RO1-HD10863"],"award-info":[{"award-number":["RO1-HD10863"]}],"id":[{"id":"10.13039\/100009633","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["www.pnas.org"],"crossmark-restriction":true},"short-container-title":["Proc. Natl. Acad. Sci. U.S.A."],"published-print":{"date-parts":[[2026,3,3]]},"abstract":"<jats:p>\n                    Nuclear Envelope Membrane Protein 1 (NEMP1) is crucial for metazoan fertility; loss of Nemp1 causes death of primordial oocytes that reside in the mechanically challenging ovarian cortex. Here, we show that softening the ovary rescues oocyte loss and restores fertility in\n                    <jats:italic toggle=\"yes\">N<\/jats:italic>\n                    <jats:italic toggle=\"yes\">emp1<\/jats:italic>\n                    <jats:italic toggle=\"yes\">knockout<\/jats:italic>\n                    <jats:italic toggle=\"yes\">(KO)<\/jats:italic>\n                    mice. In cell culture, NEMP1 depletion on stiff substrates leads to death, while cells remain viable on soft substrates. We further show that NEMP1 regulates YAP nuclear translocation, essential for mechanotransduction. Mechanistically, Nemp1-depleted cells on stiff substrates or subjected to stretching exhibit reduced nuclear YAP localization, and expressing nuclear YAP5SA restores cell viability. Loss of NEMP1 disrupts actin organization. Inducing actin polymerization partially rescues nuclear YAP, indicating a role for F-actin in NEMP1 mediated mechanotransduction. NEMP1 forms a complex with NESPRIN\u2019s Klarsicht, Anchorage (ANC)-1, Syne Homology (KASH) domain, strengthening the actin cytoskeleton to withstand mechanical forces, independent of SUN proteins. Thus, the Nemp1\u2013Nesprin complex supports a mechanosensitive pathway parallel to the LINC complex, enabling cellular response to mechanical stress in vitro and in vivo.\n                  <\/jats:p>","DOI":"10.1073\/pnas.2521253123","type":"journal-article","created":{"date-parts":[[2026,2,23]],"date-time":"2026-02-23T20:00:36Z","timestamp":1771876836000},"update-policy":"https:\/\/doi.org\/10.1073\/pnas.cm10313","source":"Crossref","is-referenced-by-count":4,"title":["The Nemp1\u2013Nesprin complex mediates cellular responses to matrix mechanics"],"prefix":"10.1073","volume":"123","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-1557-3146","authenticated-orcid":false,"given":"Abira","family":"Ganguly","sequence":"first","affiliation":[{"name":"Department of Developmental Biology, Washington University","place":["St. Louis"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hannah","family":"Zmuda","sequence":"additional","affiliation":[{"name":"Department of Biomedical Engineering, Washington University","place":["St. Louis"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Javier","family":"Abello","sequence":"additional","affiliation":[{"name":"Department of Cell Biology and Physiology, Washington University","place":["St. Louis"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Danielle","family":"Illy","sequence":"additional","affiliation":[{"name":"Department of Developmental Biology, Washington University","place":["St. Louis"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3435-5558","authenticated-orcid":false,"given":"Christopher","family":"Walter","sequence":"additional","affiliation":[{"name":"Department of Biomedical Engineering, Washington University","place":["St. Louis"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2580-0728","authenticated-orcid":false,"given":"Yonit","family":"Tsatskis","sequence":"additional","affiliation":[{"name":"The Cell Biology Program, The Hospital for Sick Children","place":["Toronto, Canada"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nattapon","family":"Thanintorn","sequence":"additional","affiliation":[{"name":"Department of Developmental Biology, Washington University","place":["St. Louis"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0007-1808-327X","authenticated-orcid":false,"given":"Ying","family":"Zhang","sequence":"additional","affiliation":[{"name":"Lunenfeld Tanenbaum Research Institute, Sinai Health System, Joseph & Wolf Lebovic Health Complex","place":["Toronto, Canada"]},{"name":"Department of Obstetrics, and Gynecology, University of Toronto","place":["Toronto, Canada"]},{"name":"Department of Physiology, University of Toronto","place":["Toronto, Canada"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0005-2794-3388","authenticated-orcid":false,"given":"Bilal Ahmad","family":"Hakim","sequence":"additional","affiliation":[{"name":"Department of Developmental Biology, Washington University","place":["St. Louis"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Didier","family":"Hodzic","sequence":"additional","affiliation":[{"name":"Department of Developmental Biology, Washington University","place":["St. Louis"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Amber N.","family":"Stratman","sequence":"additional","affiliation":[{"name":"Department of Cell Biology and Physiology, Washington University","place":["St. Louis"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Andrea","family":"Jurisicova","sequence":"additional","affiliation":[{"name":"Lunenfeld Tanenbaum Research Institute, Sinai Health System, Joseph & Wolf Lebovic Health Complex","place":["Toronto, Canada"]},{"name":"Department of Obstetrics, and Gynecology, University of Toronto","place":["Toronto, Canada"]},{"name":"Department of Physiology, University of Toronto","place":["Toronto, Canada"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4006-5119","authenticated-orcid":false,"given":"Amit","family":"Pathak","sequence":"additional","affiliation":[{"name":"Department of Biomedical Engineering, Washington University","place":["St. Louis"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1126-5154","authenticated-orcid":false,"given":"Helen","family":"McNeill","sequence":"additional","affiliation":[{"name":"Department of Developmental Biology, Washington University","place":["St. Louis"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"341","published-online":{"date-parts":[[2026,2,23]]},"reference":[{"key":"e_1_3_5_1_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.ceb.2020.03.001"},{"key":"e_1_3_5_2_2","doi-asserted-by":"publisher","DOI":"10.1038\/nature10137"},{"key":"e_1_3_5_3_2","doi-asserted-by":"publisher","DOI":"10.1158\/1078-0432.CCR-12-3172"},{"key":"e_1_3_5_4_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.yexcr.2015.10.034"},{"key":"e_1_3_5_5_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41556-018-0142-z"},{"key":"e_1_3_5_6_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.celrep.2018.10.024"},{"key":"e_1_3_5_7_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.bpj.2015.05.010"},{"key":"e_1_3_5_8_2","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.200509124"},{"key":"e_1_3_5_9_2","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.201409047"},{"key":"e_1_3_5_10_2","doi-asserted-by":"publisher","DOI":"10.1042\/BST20110686"},{"key":"e_1_3_5_11_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.cub.2013.11.009"},{"key":"e_1_3_5_12_2","doi-asserted-by":"publisher","DOI":"10.1186\/s13036-019-0197-9"},{"key":"e_1_3_5_13_2","doi-asserted-by":"publisher","DOI":"10.1038\/ncb2927"},{"key":"e_1_3_5_14_2","doi-asserted-by":"publisher","DOI":"10.1091\/mbc.E19-01-0027"},{"key":"e_1_3_5_15_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2017.10.008"},{"key":"e_1_3_5_16_2","doi-asserted-by":"publisher","DOI":"10.1080\/15476278.2015.1019687"},{"key":"e_1_3_5_17_2","doi-asserted-by":"publisher","DOI":"10.1093\/humrep\/der468"},{"key":"e_1_3_5_18_2","doi-asserted-by":"publisher","DOI":"10.1177\/1933719114542023"},{"key":"e_1_3_5_19_2","doi-asserted-by":"publisher","DOI":"10.1126\/sciadv.abb4591"},{"key":"e_1_3_5_20_2","doi-asserted-by":"publisher","DOI":"10.1007\/s10815-018-1180-y"},{"key":"e_1_3_5_21_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M115.641670"},{"key":"e_1_3_5_22_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M210144200"},{"key":"e_1_3_5_23_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1118073109"},{"key":"e_1_3_5_24_2","doi-asserted-by":"publisher","DOI":"10.1096\/fj.202300120RR"},{"key":"e_1_3_5_25_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2005.06.007"},{"key":"e_1_3_5_26_2","doi-asserted-by":"publisher","DOI":"10.1038\/ncomms7333"},{"key":"e_1_3_5_27_2","doi-asserted-by":"publisher","DOI":"10.1038\/nmeth.1401"},{"key":"e_1_3_5_28_2","doi-asserted-by":"publisher","DOI":"10.1242\/dev.070987"},{"key":"e_1_3_5_29_2","doi-asserted-by":"publisher","DOI":"10.3389\/fcell.2021.673599"},{"key":"e_1_3_5_30_2","doi-asserted-by":"publisher","DOI":"10.1161\/STROKEAHA.108.528091"},{"key":"e_1_3_5_31_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0073763"},{"key":"e_1_3_5_32_2","doi-asserted-by":"publisher","DOI":"10.1242\/jcs.209627"},{"key":"e_1_3_5_33_2","doi-asserted-by":"publisher","DOI":"10.3389\/fphys.2021.710809"},{"key":"e_1_3_5_34_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-023-37021-x"},{"key":"e_1_3_5_35_2","doi-asserted-by":"publisher","DOI":"10.1242\/jcs.057075"},{"key":"e_1_3_5_36_2","doi-asserted-by":"publisher","DOI":"10.1038\/cr.2012.126"},{"key":"e_1_3_5_37_2","doi-asserted-by":"publisher","DOI":"10.1242\/jcs.02471"},{"key":"e_1_3_5_38_2","doi-asserted-by":"publisher","DOI":"10.3389\/fcell.2022.1038107"},{"key":"e_1_3_5_39_2","doi-asserted-by":"publisher","DOI":"10.1111\/acel.13259"},{"key":"e_1_3_5_40_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.1240104"},{"key":"e_1_3_5_41_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pbio.3001811"},{"key":"e_1_3_5_42_2","doi-asserted-by":"publisher","DOI":"10.1038\/nrm3416"},{"key":"e_1_3_5_43_2","doi-asserted-by":"publisher","DOI":"10.1002\/advs.202303395"},{"key":"e_1_3_5_44_2","doi-asserted-by":"publisher","DOI":"10.1186\/s13287-024-03884-0"},{"key":"e_1_3_5_45_2","doi-asserted-by":"publisher","DOI":"10.1038\/s42003-025-07832-3"},{"key":"e_1_3_5_46_2","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.200506083"},{"key":"e_1_3_5_47_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.cub.2020.05.091"},{"key":"e_1_3_5_48_2","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.202204042"},{"key":"e_1_3_5_49_2","doi-asserted-by":"publisher","DOI":"10.1242\/jcs.226753"},{"key":"e_1_3_5_50_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.biomaterials.2017.09.012"},{"key":"e_1_3_5_51_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0914005107"},{"key":"e_1_3_5_52_2","first-page":"100141","article-title":"clusterProfiler 4.0: A universal enrichment tool for interpreting omics data","volume":"2","author":"Wu T.","year":"2021","unstructured":"T. Wu , clusterProfiler 4.0: A universal enrichment tool for interpreting omics data. Innovation 2, 100141 (2021).","journal-title":"Innovation"},{"key":"e_1_3_5_53_2","doi-asserted-by":"publisher","DOI":"10.1093\/bioinformatics\/btp616"},{"key":"e_1_3_5_54_2","doi-asserted-by":"publisher","DOI":"10.1038\/nmeth.4197"},{"key":"e_1_3_5_55_2","unstructured":"A. Ganguly The ALT-LINC complex mediates cellular responses to matrix mechanics. Gene Expression Omnibus. https:\/\/www.ncbi.nlm.nih.gov\/geo\/query\/acc.cgi?acc=GSE304914. Deposited 8 August 2025."}],"updated-by":[{"DOI":"10.1073\/pnas.2608883123","type":"correction","label":"Correction","source":"publisher","updated":{"date-parts":[[2026,4,3]],"date-time":"2026-04-03T00:00:00Z","timestamp":1775174400000}}],"container-title":["Proceedings of the National Academy of Sciences"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/pnas.org\/doi\/pdf\/10.1073\/pnas.2521253123","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,4,3]],"date-time":"2026-04-03T17:43:17Z","timestamp":1775238197000},"score":1,"resource":{"primary":{"URL":"https:\/\/pnas.org\/doi\/10.1073\/pnas.2521253123"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,2,23]]},"references-count":55,"journal-issue":{"issue":"9","published-print":{"date-parts":[[2026,3,3]]}},"alternative-id":["10.1073\/pnas.2521253123"],"URL":"https:\/\/doi.org\/10.1073\/pnas.2521253123","relation":{},"ISSN":["0027-8424","1091-6490"],"issn-type":[{"value":"0027-8424","type":"print"},{"value":"1091-6490","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,2,23]]},"assertion":[{"value":"2025-08-06","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-01-08","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-02-23","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e2521253123"}}