{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,25]],"date-time":"2026-09-25T12:18:29Z","timestamp":1790338709907,"version":"4.1.0"},"reference-count":54,"publisher":"National Academy of Sciences","issue":"49","license":[{"start":{"date-parts":[[2025,11,26]],"date-time":"2025-11-26T00:00:00Z","timestamp":1764115200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"funder":[{"DOI":"10.13039\/100015691","name":"HHS | NIH | NIAID | Division of Microbiology and Infectious Diseases","doi-asserted-by":"publisher","award":["AI153197"],"award-info":[{"award-number":["AI153197"]}],"id":[{"id":"10.13039\/100015691","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100015691","name":"HHS | NIH | NIAID | Division of Microbiology and Infectious Diseases","doi-asserted-by":"publisher","award":["T32 AI007180"],"award-info":[{"award-number":["T32 AI007180"]}],"id":[{"id":"10.13039\/100015691","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100005237","name":"Helen Hay Whitney Foundation","doi-asserted-by":"publisher","award":["NA"],"award-info":[{"award-number":["NA"]}],"id":[{"id":"10.13039\/100005237","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["www.pnas.org"],"crossmark-restriction":true},"short-container-title":["Proc. Natl. Acad. Sci. U.S.A."],"published-print":{"date-parts":[[2025,12,9]]},"abstract":"<jats:p>\n                    During\n                    <jats:italic toggle=\"yes\">Mycobacterium tuberculosis<\/jats:italic>\n                    infections bacteria are engulfed by macrophages, a main line of defense against invading pathogens. Upon activation, macrophages increase glycolysis, producing the antibacterial aldehyde methylglyoxal. To test whether bacterial methylglyoxal resistance is required for robust infections, we sought to identify\n                    <jats:italic toggle=\"yes\">M. tuberculosis<\/jats:italic>\n                    defense mechanisms against methylglyoxal. We identified\n                    <jats:italic toggle=\"yes\">phoP<\/jats:italic>\n                    mutants were among the most highly sensitive strains to methylglyoxal in vitro.\n                    <jats:italic toggle=\"yes\">phoP<\/jats:italic>\n                    mutants are highly attenuated in mice but a\n                    <jats:italic toggle=\"yes\">phoP<\/jats:italic>\n                    mutant was even more attenuated in mice that accumulate methylglyoxal. We further found\n                    <jats:italic toggle=\"yes\">phoP<\/jats:italic>\n                    bacilli were more permeable to methylglyoxal and accumulated glycated proteins. Suppressor mutations in the fatty acid \u03b2-oxidation genes\n                    <jats:italic toggle=\"yes\">fadE25<\/jats:italic>\n                    or\n                    <jats:italic toggle=\"yes\">fixB<\/jats:italic>\n                    restored impermeability and resistance to methylglyoxal to a\n                    <jats:italic toggle=\"yes\">phoP<\/jats:italic>\n                    mutant. Together, our data show that an important role for PhoP is to provide\n                    <jats:italic toggle=\"yes\">M. tuberculosis<\/jats:italic>\n                    resistance to methylglyoxal toxicity in vivo by regulating cell envelope integrity.\n                  <\/jats:p>","DOI":"10.1073\/pnas.2523024122","type":"journal-article","created":{"date-parts":[[2025,11,26]],"date-time":"2025-11-26T18:31:13Z","timestamp":1764181873000},"update-policy":"https:\/\/doi.org\/10.1073\/pnas.cm10313","source":"Crossref","is-referenced-by-count":1,"title":["Cell envelope maintenance by PhoP is essential for\n                    <i>Mycobacterium tuberculosis<\/i>\n                    methylglyoxal resistance"],"prefix":"10.1073","volume":"122","author":[{"given":"Phuong M.","family":"Tran","sequence":"first","affiliation":[{"name":"Department of Microbiology, NYU Grossman School of Medicine","place":["New York"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Andrea","family":"Anaya-Sanchez","sequence":"additional","affiliation":[{"name":"Microbiology Graduate Group, University of California","place":["Berkeley"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Daisy X.","family":"Ji","sequence":"additional","affiliation":[{"name":"Department of Microbiology, NYU Grossman School of Medicine","place":["New York"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Madeline C. R.","family":"Schwarz","sequence":"additional","affiliation":[{"name":"Mycobacteria Research Laboratories, Department of Microbiology, Immunology and Pathology, Colorado State University","place":["Fort Collins"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shiva K.","family":"Angala","sequence":"additional","affiliation":[{"name":"Mycobacteria Research Laboratories, Department of Microbiology, Immunology and Pathology, Colorado State University","place":["Fort Collins"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9212-0258","authenticated-orcid":false,"given":"Mary C.","family":"Jackson","sequence":"additional","affiliation":[{"name":"Mycobacteria Research Laboratories, Department of Microbiology, Immunology and Pathology, Colorado State University","place":["Fort Collins"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4182-9048","authenticated-orcid":false,"given":"Sarah A.","family":"Stanley","sequence":"additional","affiliation":[{"name":"Microbiology Graduate Group, University of California","place":["Berkeley"]},{"name":"Department of Molecular and Cell Biology, Division of Immunology and Pathogenesis, University of California","place":["Berkeley"]},{"name":"Division of Infectious Disease and Vaccinology, School of Public Health, University of California","place":["Berkeley"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5043-7548","authenticated-orcid":false,"given":"K. 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