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Natl. Acad. Sci. U.S.A."],"published-print":{"date-parts":[[2025,11,18]]},"abstract":"<jats:p>\n                    Carboxysomes in cyanobacteria and certain proteobacteria enable efficient CO\n                    <jats:sub>2<\/jats:sub>\n                    fixation by encapsulating ribulose-1,5-bisphosphate carboxylase\/oxygenase (Rubisco) and carbonic anhydrase (CA) within a semipermeable shell. Sequestered CA catalyze the rapid interconversion of CO\n                    <jats:sub>2<\/jats:sub>\n                    and HCO\n                    <jats:sub>3<\/jats:sub>\n                    <jats:sup>\u2212<\/jats:sup>\n                    , supplying elevated levels of CO\n                    <jats:sub>2<\/jats:sub>\n                    to boost Rubisco carboxylation. Despite its essential role, the structure and encapsulation of CA within carboxysomes remain poorly understood. Here, we determined the molecular structure of \u03b1-carboxysomal CA from the model chemoautotrophic bacterium\n                    <jats:italic toggle=\"yes\">Halothiobacillus neapolitanus<\/jats:italic>\n                    (\n                    <jats:italic toggle=\"yes\">Hn<\/jats:italic>\n                    CsoSCA).\n                    <jats:italic toggle=\"yes\">Hn<\/jats:italic>\n                    CsoSCA adopts a trimer-of-dimers oligomeric structure without the incorporation of a zinc ion at its symmetric center. Using synthetic minishells, we demonstrate that\n                    <jats:italic toggle=\"yes\">Hn<\/jats:italic>\n                    CsoSCA interacts with the CsoS1A shell hexamer and is incorporated into the minishells at the inner surface, independent of the CsoS2 linker protein.\n                    <jats:italic toggle=\"yes\">Hn<\/jats:italic>\n                    CsoSCA truncations suggest nonspecific interactions between\n                    <jats:italic toggle=\"yes\">Hn<\/jats:italic>\n                    CsoSCA and CsoS1A. We further show that\n                    <jats:italic toggle=\"yes\">Hn<\/jats:italic>\n                    CsoSCA bridges Rubisco and the shell facets. Our study offers insights into the assembly and encapsulation mechanisms of \u03b1-carboxysomes and provides the framework for reprogramming carboxysome structures for synthetic biology and biotechnological applications.\n                  <\/jats:p>","DOI":"10.1073\/pnas.2523723122","type":"journal-article","created":{"date-parts":[[2025,11,12]],"date-time":"2025-11-12T18:40:57Z","timestamp":1762972857000},"update-policy":"https:\/\/doi.org\/10.1073\/pnas.cm10313","source":"Crossref","is-referenced-by-count":6,"title":["Structure and encapsulation of carbonic anhydrase within the \u03b1-carboxysome"],"prefix":"10.1073","volume":"122","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-0905-0584","authenticated-orcid":false,"given":"Pei Cing","family":"Ng","sequence":"first","affiliation":[{"name":"Institute of Systems, Molecular and Integrative Biology, University of Liverpool","place":["Liverpool, United 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