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The lysines modified in free RT but protected from biotinylation in the nucleoprotein complex were readily revealed by this approach. Results of a control experiment examining the RT-DNA:DNA complex were in excellent agreement with the crystal structure data on the identical complex. Probing the RT-viral RNA:tRNA complex revealed that a majority of protein contacts are located in the primer-template binding cleft in common with the RT-DNA:DNA and RT-RNA:DNA species. However, our footprinting data indicate that the p66 fingers subdomain makes additional contacts to the viral RNA:tRNA specific for this complex and not detected with DNA:DNA. The protein footprinting method described herein has a generic application for high-resolution solution structural studies of multiprotein-nucleic acid contacts.<\/jats:p>","DOI":"10.1073\/pnas.252550199","type":"journal-article","created":{"date-parts":[[2002,12,10]],"date-time":"2002-12-10T12:42:57Z","timestamp":1039524177000},"page":"15988-15993","update-policy":"https:\/\/doi.org\/10.1073\/pnas.cm10313","source":"Crossref","is-referenced-by-count":53,"title":["Identification of specific HIV-1 reverse transcriptase contacts to the viral RNA:tRNA complex by mass spectrometry and a primary amine selective reagent"],"prefix":"10.1073","volume":"99","author":[{"given":"Mamuka","family":"Kvaratskhelia","sequence":"first","affiliation":[{"name":"HIV Drug Resistance Program, National Cancer Institute, Frederick, MD 21702; and National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, Bethesda, MD 20892-0805"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jennifer T.","family":"Miller","sequence":"additional","affiliation":[{"name":"HIV Drug Resistance Program, National Cancer Institute, Frederick, MD 21702; and National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, Bethesda, MD 20892-0805"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Scott R.","family":"Budihas","sequence":"additional","affiliation":[{"name":"HIV Drug Resistance Program, National Cancer Institute, Frederick, MD 21702; and National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, Bethesda, MD 20892-0805"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lewis K.","family":"Pannell","sequence":"additional","affiliation":[{"name":"HIV Drug Resistance Program, National Cancer Institute, Frederick, MD 21702; and National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, Bethesda, MD 20892-0805"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Stuart F. 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