{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,19]],"date-time":"2026-09-19T18:00:08Z","timestamp":1789840808311,"version":"4.0.1"},"reference-count":26,"publisher":"National Academy of Sciences","issue":"3","content-domain":{"domain":["www.pnas.org"],"crossmark-restriction":true},"short-container-title":["Proc. Natl. Acad. Sci. U.S.A."],"published-print":{"date-parts":[[2003,2,4]]},"abstract":"<jats:p>\n                    Gene expression profiling was carried out comparing Con A elicited peritoneal macrophages from C57BL\/6 and FVB\/N wild-type and apolipoprotein (apo)E knockout mice. An EST,\n                    <jats:ext-link xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" ext-link-type=\"gen\" xlink:href=\"W20829\">W20829<\/jats:ext-link>\n                    , was expressed at higher levels in C57BL\/6 compared with FVB\/N mice.\n                    <jats:ext-link xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" ext-link-type=\"gen\" xlink:href=\"W20829\">W20829<\/jats:ext-link>\n                    mapped to an atherosclerosis susceptibility locus on chromosome 19 revealed in an intercross between atherosclerosis-susceptible C57BL\/6 and atherosclerosis-resistant FVB\/N apoE knockout mice. A combination of database search and Northern analysis confirmed that\n                    <jats:ext-link xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" ext-link-type=\"gen\" xlink:href=\"W20829\">W20829<\/jats:ext-link>\n                    corresponded to 3\u2032-UTR of a hitherto predicted gene, named\n                    <jats:italic>HspA12A<\/jats:italic>\n                    . Blasting the National Center for Biotechnology Information database revealed a closely related homologue,\n                    <jats:italic>HspA12B<\/jats:italic>\n                    . HspA12A and -B have very close human homologues. TaqMan analysis confirmed the increased\n                    <jats:italic>HspA12A<\/jats:italic>\n                    expression (2.6-fold) in elicited peritoneal macrophages from C57BL\/6 compared with FVB\/N mice. TaqMan analysis also revealed increased\n                    <jats:italic>HspA12A<\/jats:italic>\n                    and\n                    <jats:italic>HspA12B<\/jats:italic>\n                    expression (87- and 6-fold, respectively) in lesional versus nonlesional portions of the thoracic aorta from C57BL\/6 apoE knockout mice on a chow diet.\n                    <jats:italic>In situ<\/jats:italic>\n                    hybridization confirmed that both genes were expressed within lesions but not within nonlesional aortic tissue. Blasting of HspA12A and HspA12B against the National Center for Biotechnology Information database (NR) revealed a hit with the Conserved Domain database for Hsp70 (pfam00012.5, Hsp70). Both genes appear to contain an atypical Hsp70 ATPase domain. The BLAST search also revealed that both genes were more similar to primitive eukaryote and prokaryote than mammalian Hsp70s, making these two genes distant members of the mammalian Hsp70 family. In summary, we describe two genes that code for a subfamily of Hsp70 proteins that may be involved in atherosclerosis susceptibility.\n                  <\/jats:p>","DOI":"10.1073\/pnas.252764399","type":"journal-article","created":{"date-parts":[[2003,2,4]],"date-time":"2003-02-04T12:24:41Z","timestamp":1044361481000},"page":"1256-1261","update-policy":"https:\/\/doi.org\/10.1073\/pnas.cm10313","source":"Crossref","is-referenced-by-count":109,"title":["Two Hsp70 family members expressed in atherosclerotic lesions"],"prefix":"10.1073","volume":"100","author":[{"given":"Zhihua","family":"Han","sequence":"first","affiliation":[{"name":"Laboratory of Biochemical Genetics and Metabolism, The Rockefeller University, 1230 York Avenue, New York, NY 10021"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Quynh A.","family":"Truong","sequence":"additional","affiliation":[{"name":"Laboratory of Biochemical Genetics and Metabolism, The Rockefeller University, 1230 York Avenue, New York, NY 10021"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shirley","family":"Park","sequence":"additional","affiliation":[{"name":"Laboratory of Biochemical Genetics and Metabolism, The Rockefeller University, 1230 York Avenue, New York, NY 10021"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jan L.","family":"Breslow","sequence":"additional","affiliation":[{"name":"Laboratory of Biochemical Genetics and Metabolism, The Rockefeller University, 1230 York Avenue, New York, NY 10021"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"341","published-online":{"date-parts":[[2003,1,27]]},"reference":[{"key":"e_1_3_4_1_2","doi-asserted-by":"crossref","first-page":"343","DOI":"10.1016\/0092-8674(92)90362-G","volume":"71","author":"Plump A S","year":"1992","unstructured":"A S Plump, J D Smith, T Hayek, K Aalto-Setala, A Walsh, J G Verstuyft, E M Rubin, J L Breslow Cell 71, 343\u2013353 (1992).","journal-title":"Cell"},{"key":"e_1_3_4_2_2","doi-asserted-by":"crossref","first-page":"468","DOI":"10.1126\/science.1411543","volume":"258","author":"Zhang S H","year":"1992","unstructured":"S H Zhang, R L Reddick, J A Piedrahita, N Maeda Science 258, 468\u2013471 (1992).","journal-title":"Science"},{"key":"e_1_3_4_3_2","doi-asserted-by":"crossref","first-page":"8264","DOI":"10.1073\/pnas.92.18.8264","volume":"92","author":"Smith J D","year":"1995","unstructured":"J D Smith, E Trogan, M Ginsberg, C Grigaux, J Tian, M Miyata Proc Natl Acad Sci USA 92, 8264\u20138268 (1995).","journal-title":"Proc Natl Acad Sci USA"},{"key":"e_1_3_4_4_2","doi-asserted-by":"crossref","first-page":"894","DOI":"10.1038\/29788","volume":"394","author":"Boring L","year":"1998","unstructured":"L Boring, J Gosling, M Cleary, I F Charo Nature 394, 894\u2013897 (1998).","journal-title":"Nature"},{"key":"e_1_3_4_5_2","doi-asserted-by":"crossref","first-page":"205","DOI":"10.1016\/S0021-9150(98)00318-9","volume":"143","author":"Dawson T C","year":"1999","unstructured":"T C Dawson, W A Kuziel, T A Osahar, N Maeda Atherosclerosis 143, 205\u2013211 (1999).","journal-title":"Atherosclerosis"},{"key":"e_1_3_4_6_2","doi-asserted-by":"crossref","first-page":"1255","DOI":"10.1172\/JCI11871","volume":"107","author":"Cybulsky M I","year":"2001","unstructured":"M I Cybulsky, K Iiyama, H Li, S Zhu, M Chen, M Iiyama, V Davis, J C Gutierrez-Ramos, P W Connelly, D S Milstone J Clin Invest 107, 1255\u20131262 (2001).","journal-title":"J Clin Invest"},{"key":"e_1_3_4_7_2","doi-asserted-by":"crossref","first-page":"1662","DOI":"10.1161\/hq1001.096625","volume":"21","author":"Dansky H M","year":"2001","unstructured":"H M Dansky, C B Barlow, C Lominska, J L Sikes, C Kao, J Weinsaft, M I Cybulsky, J D Smith Arterioscler Thromb Vasc Biol 21, 1662\u20131667 (2001).","journal-title":"Arterioscler Thromb Vasc Biol"},{"key":"e_1_3_4_8_2","doi-asserted-by":"crossref","first-page":"839","DOI":"10.1038\/ncb0901-839","volume":"3","author":"Ravagnan L","year":"2001","unstructured":"L Ravagnan, S Gurbuxani, S A Susin, C Maisse, E Daugas, N Zamzami, T Mak, M Jaattela, J M Penninger, C Garrido, G Kroemer Nat Cell Biol 3, 839\u2013843 (2001).","journal-title":"Nat Cell Biol"},{"key":"e_1_3_4_9_2","doi-asserted-by":"crossref","first-page":"65","DOI":"10.1016\/0021-9150(85)90138-8","volume":"57","author":"Paigen B","year":"1985","unstructured":"B Paigen, A Morrow, C Brandon, D Mitchell, P Holmes Atherosclerosis 57, 65\u201373 (1985).","journal-title":"Atherosclerosis"},{"key":"e_1_3_4_10_2","doi-asserted-by":"crossref","first-page":"1599","DOI":"10.1093\/genetics\/160.4.1599","volume":"160","author":"Dansky H M","year":"2002","unstructured":"H M Dansky, P Shu, M Donavan, J Montagno, D L Nagle, J S Smutko, N Roy, S Whiteing, J Barrios, T J McBride, et al. 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