{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,3]],"date-time":"2026-08-03T20:01:55Z","timestamp":1785787315365,"version":"3.56.0"},"reference-count":55,"publisher":"National Academy of Sciences","issue":"32","license":[{"start":{"date-parts":[[2026,8,3]],"date-time":"2026-08-03T00:00:00Z","timestamp":1785715200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"funder":[{"DOI":"10.13039\/100000060","name":"HHS | NIH | National Institute of Allergy and Infectious Diseases","doi-asserted-by":"publisher","award":["UM1 AI164570"],"award-info":[{"award-number":["UM1 AI164570"]}],"id":[{"id":"10.13039\/100000060","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000060","name":"HHS | NIH | National Institute of Allergy and Infectious Diseases","doi-asserted-by":"publisher","award":["UM1AI164556"],"award-info":[{"award-number":["UM1AI164556"]}],"id":[{"id":"10.13039\/100000060","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000060","name":"HHS | NIH | National Institute of Allergy and Infectious Diseases","doi-asserted-by":"publisher","award":["UM1AI191272"],"award-info":[{"award-number":["UM1AI191272"]}],"id":[{"id":"10.13039\/100000060","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000060","name":"HHS | NIH | National Institute of Allergy and Infectious Diseases","doi-asserted-by":"publisher","award":["UM1AI164560"],"award-info":[{"award-number":["UM1AI164560"]}],"id":[{"id":"10.13039\/100000060","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000060","name":"HHS | NIH | National Institute of Allergy and Infectious Diseases","doi-asserted-by":"publisher","award":["P30AI045008"],"award-info":[{"award-number":["P30AI045008"]}],"id":[{"id":"10.13039\/100000060","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["www.pnas.org"],"crossmark-restriction":true},"short-container-title":["Proc. Natl. Acad. Sci. U.S.A."],"published-print":{"date-parts":[[2026,8,11]]},"abstract":"<jats:p>\n                    Bispecific antibodies that reroute cytotoxic effectors toward infected cells are promising HIV-1 cure agents, yet existing formats bind Env and are limited by antigenic variation and Env down-regulation. We engineered a TCR-mimic single-chain diabody, HI12, that recognizes a conserved Pol-derived peptide presented by HLA-A*02:01 and evaluated its effect in HLA-matched, HIV-infected humanized mice. When administered during early antiretroviral therapy (ART), HI12 was well tolerated, activated CD8\n                    <jats:sup>+<\/jats:sup>\n                    T cells, and accelerated plasma virus decay. Treatment produced three- to eightfold reductions in intact and total proviral DNA within lymph-node and splenic CD4\n                    <jats:sup>+<\/jats:sup>\n                    T cells, indicating substantive reservoir clearance. After ART interruption, HI12-treated animals showed a significant delay in viral rebound compared with controls, linking reservoir reduction to improved posttherapy control. These findings provide in vivo evidence that a peptide-HLA-directed bispecific antibody can both shrink the intact HIV reservoir and defer viral recrudescence, supporting further development of TCR-mimic bispecific antibodies for cure strategies.\n                  <\/jats:p>","DOI":"10.1073\/pnas.2527880123","type":"journal-article","created":{"date-parts":[[2026,8,3]],"date-time":"2026-08-03T19:06:16Z","timestamp":1785783976000},"update-policy":"https:\/\/doi.org\/10.1073\/pnas.cm10313","source":"Crossref","is-referenced-by-count":0,"title":["A TCR-mimic bispecific antibody reduces HIV-1 provirus and delays viral rebound in HLA-matched humanized mice"],"prefix":"10.1073","volume":"123","author":[{"given":"Zhe","family":"Yuan","sequence":"first","affiliation":[{"id":[{"id":"https:\/\/ror.org\/04wncat98","id-type":"ROR","asserted-by":"publisher"}],"name":"The Wistar Institute","place":["Philadelphia"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5208-9970","authenticated-orcid":false,"given":"Nathan L.","family":"Board","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/00za53h95","id-type":"ROR","asserted-by":"publisher"}],"name":"Department of Medicine, The Johns Hopkins University School of Medicine","place":["Baltimore"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Miaoyun","family":"Zhao","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/04wncat98","id-type":"ROR","asserted-by":"publisher"}],"name":"The Wistar Institute","place":["Philadelphia"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Guorui","family":"Zu","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/04wncat98","id-type":"ROR","asserted-by":"publisher"}],"name":"The Wistar Institute","place":["Philadelphia"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Srona","family":"Sengupta","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/00za53h95","id-type":"ROR","asserted-by":"publisher"}],"name":"Department of Medicine, The Johns Hopkins University School of Medicine","place":["Baltimore"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qingsheng","family":"Li","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/04wncat98","id-type":"ROR","asserted-by":"publisher"}],"name":"The Wistar Institute","place":["Philadelphia"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Janet D.","family":"Siliciano","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/00za53h95","id-type":"ROR","asserted-by":"publisher"}],"name":"Department of Medicine, The Johns Hopkins University School of Medicine","place":["Baltimore"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1764-1993","authenticated-orcid":false,"given":"Robert F.","family":"Siliciano","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/00za53h95","id-type":"ROR","asserted-by":"publisher"}],"name":"Department of Medicine, The Johns Hopkins University School of Medicine","place":["Baltimore"]},{"name":"HHMI","place":["Baltimore"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5799-6759","authenticated-orcid":false,"given":"Luis J.","family":"Montaner","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/04wncat98","id-type":"ROR","asserted-by":"publisher"}],"name":"The Wistar Institute","place":["Philadelphia"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"341","published-online":{"date-parts":[[2026,8,3]]},"reference":[{"key":"e_1_3_4_1_2","doi-asserted-by":"publisher","DOI":"10.1038\/387188a0"},{"key":"e_1_3_4_2_2","doi-asserted-by":"publisher","DOI":"10.1086\/314623"},{"key":"e_1_3_4_3_2","doi-asserted-by":"publisher","DOI":"10.1038\/nm1295-1284"},{"key":"e_1_3_4_4_2","doi-asserted-by":"publisher","DOI":"10.1038\/387183a0"},{"key":"e_1_3_4_5_2","doi-asserted-by":"publisher","DOI":"10.1038\/nm880"},{"key":"e_1_3_4_6_2","doi-asserted-by":"publisher","DOI":"10.1172\/jci.insight.132997"},{"key":"e_1_3_4_7_2","doi-asserted-by":"publisher","DOI":"10.1172\/JCI171554"},{"key":"e_1_3_4_8_2","doi-asserted-by":"publisher","DOI":"10.1172\/JCI82314"},{"key":"e_1_3_4_9_2","doi-asserted-by":"publisher","DOI":"10.1038\/ncomms9447"},{"key":"e_1_3_4_10_2","doi-asserted-by":"publisher","DOI":"10.3389\/fimmu.2020.00713"},{"key":"e_1_3_4_11_2","doi-asserted-by":"publisher","DOI":"10.1097\/QAD.0000000000002543"},{"key":"e_1_3_4_12_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.2123406119"},{"key":"e_1_3_4_13_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41590-023-01741-5"},{"key":"e_1_3_4_14_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.abc8697"},{"key":"e_1_3_4_15_2","doi-asserted-by":"publisher","DOI":"10.1126\/sciimmunol.abd5515"},{"key":"e_1_3_4_16_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41586-019-0898-8"},{"key":"e_1_3_4_17_2","doi-asserted-by":"publisher","DOI":"10.20411\/pai.v1i1.100"},{"key":"e_1_3_4_18_2","doi-asserted-by":"publisher","DOI":"10.1084\/jem.20090365"},{"key":"e_1_3_4_19_2","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.00182-09"},{"key":"e_1_3_4_20_2","doi-asserted-by":"publisher","DOI":"10.1038\/nm992"},{"key":"e_1_3_4_21_2","doi-asserted-by":"publisher","DOI":"10.1038\/nri1417"},{"key":"e_1_3_4_22_2","doi-asserted-by":"publisher","DOI":"10.1038\/nature14053"},{"key":"e_1_3_4_23_2","first-page":"661","article-title":"The effect of interference on the CD8(+) T cell escape rates in HIV","volume":"5","author":"Garcia V.","year":"2014","unstructured":"V. Garcia, R. R. Regoes, The effect of interference on the CD8(+) T cell escape rates in HIV. Front. Immunol. 5, 661 (2014).","journal-title":"Front. Immunol."},{"key":"e_1_3_4_24_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pcbi.1004721"},{"key":"e_1_3_4_25_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.ppat.1005233"},{"key":"e_1_3_4_26_2","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.00491-18"},{"key":"e_1_3_4_27_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.ppat.1003461"},{"key":"e_1_3_4_28_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.aav5095"},{"key":"e_1_3_4_29_2","doi-asserted-by":"publisher","DOI":"10.1172\/JCI44932"},{"key":"e_1_3_4_30_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2013.09.020"},{"key":"e_1_3_4_31_2","doi-asserted-by":"publisher","DOI":"10.1038\/nm.4156"},{"key":"e_1_3_4_32_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.chom.2017.03.008"},{"key":"e_1_3_4_33_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1917876117"},{"key":"e_1_3_4_34_2","doi-asserted-by":"publisher","DOI":"10.1172\/JCI165245"},{"key":"e_1_3_4_35_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1705301114"},{"key":"e_1_3_4_36_2","doi-asserted-by":"publisher","DOI":"10.1172\/JCI162694"},{"key":"e_1_3_4_37_2","doi-asserted-by":"publisher","DOI":"10.3390\/microorganisms11081984"},{"key":"e_1_3_4_38_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M109.027078"},{"key":"e_1_3_4_39_2","doi-asserted-by":"publisher","DOI":"10.1093\/protein\/gzr061"},{"key":"e_1_3_4_40_2","doi-asserted-by":"publisher","DOI":"10.1097\/COH.0000000000000293"},{"key":"e_1_3_4_41_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1318249111"},{"key":"e_1_3_4_42_2","doi-asserted-by":"publisher","DOI":"10.1124\/jpet.119.259150"},{"key":"e_1_3_4_43_2","doi-asserted-by":"publisher","DOI":"10.1126\/scitranslmed.aap8758"},{"key":"e_1_3_4_44_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.01384-20"},{"key":"e_1_3_4_45_2","doi-asserted-by":"publisher","DOI":"10.1097\/QAD.0000000000003201"},{"key":"e_1_3_4_46_2","doi-asserted-by":"publisher","DOI":"10.1186\/s12977-016-0268-7"},{"key":"e_1_3_4_47_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0211746"},{"key":"e_1_3_4_48_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41586-020-1951-3"},{"key":"e_1_3_4_49_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41586-020-1946-0"},{"key":"e_1_3_4_50_2","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.00860-16"},{"key":"e_1_3_4_51_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41426-018-0062-9"},{"key":"e_1_3_4_52_2","doi-asserted-by":"publisher","DOI":"10.1097\/QAD.0000000000003443"},{"key":"e_1_3_4_53_2","doi-asserted-by":"publisher","DOI":"10.1021\/acssynbio.6b00373"},{"key":"e_1_3_4_54_2","doi-asserted-by":"publisher","DOI":"10.1016\/S2055-6640(20)30298-3"},{"key":"e_1_3_4_55_2","doi-asserted-by":"publisher","DOI":"10.1007\/s11481-018-9809-2"}],"container-title":["Proceedings of the National Academy of Sciences"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/pnas.org\/doi\/pdf\/10.1073\/pnas.2527880123","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,8,3]],"date-time":"2026-08-03T19:06:46Z","timestamp":1785784006000},"score":1,"resource":{"primary":{"URL":"https:\/\/pnas.org\/doi\/10.1073\/pnas.2527880123"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,8,3]]},"references-count":55,"journal-issue":{"issue":"32","published-print":{"date-parts":[[2026,8,11]]}},"alternative-id":["10.1073\/pnas.2527880123"],"URL":"https:\/\/doi.org\/10.1073\/pnas.2527880123","relation":{},"ISSN":["0027-8424","1091-6490"],"issn-type":[{"value":"0027-8424","type":"print"},{"value":"1091-6490","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,8,3]]},"assertion":[{"value":"2025-10-02","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-06-29","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-08-03","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e2527880123"}}