{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,8]],"date-time":"2026-09-08T18:10:00Z","timestamp":1788891000139,"version":"build-2803163510"},"reference-count":90,"publisher":"National Academy of Sciences","issue":"37","license":[{"start":{"date-parts":[[2026,9,8]],"date-time":"2026-09-08T00:00:00Z","timestamp":1788825600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001665","name":"Agence Nationale de la Recherche","doi-asserted-by":"publisher","award":["PRC AAPG2022 NEW-corset"],"award-info":[{"award-number":["PRC AAPG2022 NEW-corset"]}],"id":[{"id":"10.13039\/501100001665","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001665","name":"Agence Nationale de la Recherche","doi-asserted-by":"publisher","award":["PRC AAPG2022 ForcesOnCell"],"award-info":[{"award-number":["PRC AAPG2022 ForcesOnCell"]}],"id":[{"id":"10.13039\/501100001665","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"MOST | National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["NSFC 12402073"],"award-info":[{"award-number":["NSFC 12402073"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100004543","name":"China Scholarship Council","doi-asserted-by":"publisher","award":["201909650006"],"award-info":[{"award-number":["201909650006"]}],"id":[{"id":"10.13039\/501100004543","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100004543","name":"China Scholarship Council","doi-asserted-by":"publisher","award":["202190850021"],"award-info":[{"award-number":["202190850021"]}],"id":[{"id":"10.13039\/501100004543","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100004097","name":"Fondation ARC pour la Recherche sur le Cancer","doi-asserted-by":"publisher","award":["AAP 4th"],"award-info":[{"award-number":["AAP 4th"]}],"id":[{"id":"10.13039\/501100004097","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["www.pnas.org"],"crossmark-restriction":true},"short-container-title":["Proc. Natl. Acad. Sci. U.S.A."],"published-print":{"date-parts":[[2026,9,15]]},"abstract":"<jats:p>\n                    Pulsatile actomyosin networks emerge as a widespread mechanism driving tissue morphogenesis. Compared with myosin-II pulses, F-actin pulses are largely unexplored. By studying\n                    <jats:italic toggle=\"yes\">Drosophila<\/jats:italic>\n                    oogenesis, we report that basal pulsatile actomyosin networks consist of F-actin bundle and meshwork architectures, implicating the existence of different F-actin microstructures and corresponding nucleators. Here, Rac1 and the Scar\/WAVE-Arp2\/3 complex (branched F-actin nucleator), exhibiting constant levels, are necessary to support F-actin pulsation, whereas pulses of Dia (unbranched F-actin nucleator) trigger F-actin pulsation. The pulsatile F-actin networks recruit the F-actin turnover regulator cofilin, thus generating cofilin pulses. Cofilin at relatively low concentration can cooperate with F-actin nucleators to amplify F-actin and enhance its pulsation, while cofilin at relatively high concentration might trigger the F-actin disassembly to attenuate its pulsation. These two different effects of cofilin on F-actin pulsation were confirmed by Zdk-cofilin optogenetics. Rac1 signaling, Dia, and cofilin are also crucial for F-actin pulses in border cell migration. Our findings thus reveal a coordinated regulation of F-actin pulses conferring actomyosin contractility in morphogenesis and cell migration.\n                  <\/jats:p>","DOI":"10.1073\/pnas.2530056123","type":"journal-article","created":{"date-parts":[[2026,9,8]],"date-time":"2026-09-08T17:47:37Z","timestamp":1788889657000},"update-policy":"https:\/\/doi.org\/10.1073\/pnas.cm10313","source":"Crossref","is-referenced-by-count":0,"title":["Coordinated regulation of diverse F-actin organizations orchestrates F-actin pulses in the actomyosin network"],"prefix":"10.1073","volume":"123","author":[{"ORCID":"https:\/\/orcid.org\/0009-0008-4834-1185","authenticated-orcid":false,"given":"Hao","family":"Li","sequence":"first","affiliation":[{"id":[{"id":"https:\/\/ror.org\/01ahyrz84","id-type":"ROR","asserted-by":"publisher"}],"name":"CNRS, Centre de Biologie Integrative, Molecular Cellular and Developmental Biology Unit, University of Toulouse","place":["Toulouse, France"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8897-4165","authenticated-orcid":false,"given":"Bing","family":"Liu","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/01ahyrz84","id-type":"ROR","asserted-by":"publisher"}],"name":"CNRS, Centre de Biologie Integrative, Molecular Cellular and Developmental Biology Unit, University of Toulouse","place":["Toulouse, France"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0649-4710","authenticated-orcid":false,"given":"Jiaying","family":"Liu","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/01ahyrz84","id-type":"ROR","asserted-by":"publisher"}],"name":"CNRS, Centre de Biologie Integrative, Molecular Cellular and Developmental Biology Unit, University of Toulouse","place":["Toulouse, France"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Thomas","family":"Mangeat","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/01ahyrz84","id-type":"ROR","asserted-by":"publisher"}],"name":"CNRS, Centre de Biologie Integrative, Molecular Cellular and Developmental Biology Unit, University of Toulouse","place":["Toulouse, France"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Karine","family":"Belguise","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/01ahyrz84","id-type":"ROR","asserted-by":"publisher"}],"name":"CNRS, Centre de Biologie Integrative, Molecular Cellular and Developmental Biology Unit, University of Toulouse","place":["Toulouse, France"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shi-Lei","family":"Xue","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/05hfa4n20","id-type":"ROR","asserted-by":"publisher"}],"name":"Department of Materials Science and Engineering, School of Engineering, Westlake University","place":["Hangzhou, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3311-1378","authenticated-orcid":false,"given":"Xiaobo","family":"Wang","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/01ahyrz84","id-type":"ROR","asserted-by":"publisher"}],"name":"CNRS, Centre de Biologie Integrative, Molecular Cellular and Developmental Biology Unit, University of Toulouse","place":["Toulouse, France"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"341","published-online":{"date-parts":[[2026,9,8]]},"reference":[{"key":"e_1_3_4_1_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2013.05.008"},{"key":"e_1_3_4_2_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.ceb.2010.10.014"},{"key":"e_1_3_4_3_2","doi-asserted-by":"publisher","DOI":"10.1038\/nrm2786"},{"key":"e_1_3_4_4_2","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.cb.03.110187.002115"},{"key":"e_1_3_4_5_2","doi-asserted-by":"publisher","DOI":"10.1242\/dev.01938"},{"key":"e_1_3_4_6_2","first-page":"2153","article-title":"Apical constriction is driven by a pulsatile apical myosin network in delaminating Drosophila neuroblasts","volume":"144","author":"An Y.","year":"2017","unstructured":"Y. An , Apical constriction is driven by a pulsatile apical myosin network in delaminating Drosophila neuroblasts. Development 144, 2153\u20132164 (2017).","journal-title":"Development"},{"key":"e_1_3_4_7_2","doi-asserted-by":"publisher","DOI":"10.1002\/dvdy.24363"},{"key":"e_1_3_4_8_2","doi-asserted-by":"publisher","DOI":"10.1038\/ncb2124"},{"key":"e_1_3_4_9_2","doi-asserted-by":"publisher","DOI":"10.1242\/dev.146761"},{"key":"e_1_3_4_10_2","doi-asserted-by":"publisher","DOI":"10.1242\/jcs.067579"},{"key":"e_1_3_4_11_2","doi-asserted-by":"publisher","DOI":"10.1038\/ncb3185"},{"key":"e_1_3_4_12_2","doi-asserted-by":"publisher","DOI":"10.1038\/nature07522"},{"key":"e_1_3_4_13_2","doi-asserted-by":"publisher","DOI":"10.1038\/nature09566"},{"key":"e_1_3_4_14_2","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.201608038"},{"key":"e_1_3_4_15_2","doi-asserted-by":"publisher","DOI":"10.1242\/dev.014704"},{"key":"e_1_3_4_16_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2009.03.050"},{"key":"e_1_3_4_17_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-021-21372-4"},{"key":"e_1_3_4_18_2","doi-asserted-by":"publisher","DOI":"10.7554\/eLife.84019"},{"key":"e_1_3_4_19_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1400520111"},{"key":"e_1_3_4_20_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-018-03574-5"},{"key":"e_1_3_4_21_2","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.201402004"},{"key":"e_1_3_4_22_2","doi-asserted-by":"publisher","DOI":"10.1038\/nature14603"},{"key":"e_1_3_4_23_2","doi-asserted-by":"publisher","DOI":"10.1038\/ncb2205"},{"key":"e_1_3_4_24_2","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.200511093"},{"key":"e_1_3_4_25_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.bpj.2013.05.027"},{"key":"e_1_3_4_26_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.ydbio.2009.10.031"},{"key":"e_1_3_4_27_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1316572110"},{"key":"e_1_3_4_28_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-022-28961-x"},{"key":"e_1_3_4_29_2","doi-asserted-by":"publisher","DOI":"10.1101\/cshperspect.a018267"},{"key":"e_1_3_4_30_2","doi-asserted-by":"publisher","DOI":"10.1038\/ncb3251"},{"key":"e_1_3_4_31_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41556-020-0524-x"},{"key":"e_1_3_4_32_2","doi-asserted-by":"publisher","DOI":"10.4161\/sgtp.29513"},{"key":"e_1_3_4_33_2","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.biophys.35.040405.101936"},{"key":"e_1_3_4_34_2","doi-asserted-by":"publisher","DOI":"10.1038\/nrm2476"},{"key":"e_1_3_4_35_2","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.202309021"},{"key":"e_1_3_4_36_2","doi-asserted-by":"publisher","DOI":"10.3389\/fcell.2021.645235"},{"key":"e_1_3_4_37_2","doi-asserted-by":"publisher","DOI":"10.1038\/ncomms14708"},{"key":"e_1_3_4_38_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-020-15593-2"},{"key":"e_1_3_4_39_2","first-page":"1855","article-title":"Actin stress fibers\u2013assembly, dynamics and biological roles","volume":"125","author":"Tojkander S.","year":"2012","unstructured":"S. Tojkander, G. Gateva, P. Lappalainen, Actin stress fibers\u2013assembly, dynamics and biological roles. J. Cell Sci. 125, 1855\u20131864 (2012).","journal-title":"J. Cell Sci."},{"key":"e_1_3_4_40_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.crmeth.2021.100009"},{"key":"e_1_3_4_41_2","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.133.6.1403"},{"key":"e_1_3_4_42_2","doi-asserted-by":"publisher","DOI":"10.4161\/sgtp.27539"},{"key":"e_1_3_4_43_2","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.133.3.617"},{"key":"e_1_3_4_44_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.cub.2019.07.035"},{"key":"e_1_3_4_45_2","doi-asserted-by":"publisher","DOI":"10.1038\/sj.emboj.7600084"},{"key":"e_1_3_4_46_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.devcel.2022.10.004"},{"key":"e_1_3_4_47_2","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.200709092"},{"key":"e_1_3_4_48_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-022-33727-6"},{"key":"e_1_3_4_49_2","doi-asserted-by":"publisher","DOI":"10.1039\/c4ib00099d"},{"key":"e_1_3_4_50_2","doi-asserted-by":"publisher","DOI":"10.1080\/21541248.2016.1224399"},{"key":"e_1_3_4_51_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.tice.2020.101364"},{"key":"e_1_3_4_52_2","doi-asserted-by":"publisher","DOI":"10.1038\/nature08242"},{"key":"e_1_3_4_53_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.cub.2013.05.005"},{"key":"e_1_3_4_54_2","doi-asserted-by":"publisher","DOI":"10.1242\/jcs.01211"},{"key":"e_1_3_4_55_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0960-9822(01)00629-7"},{"key":"e_1_3_4_56_2","doi-asserted-by":"publisher","DOI":"10.1038\/nrm3609"},{"key":"e_1_3_4_57_2","doi-asserted-by":"publisher","DOI":"10.1038\/12963"},{"key":"e_1_3_4_58_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.285.5429.895"},{"key":"e_1_3_4_59_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.cub.2018.07.082"},{"key":"e_1_3_4_60_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.275.7.5163"},{"key":"e_1_3_4_61_2","first-page":"3796","article-title":"Initiation of lamellipodia and ruffles involves cooperation between mDia1 and the Arp2\/3 complex","volume":"128","author":"Isogai T.","year":"2015","unstructured":"T. Isogai , Initiation of lamellipodia and ruffles involves cooperation between mDia1 and the Arp2\/3 complex. J. Cell Sci. 128, 3796\u20133810 (2015).","journal-title":"J. Cell Sci."},{"key":"e_1_3_4_62_2","doi-asserted-by":"publisher","DOI":"10.1529\/biophysj.106.080937"},{"key":"e_1_3_4_63_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.cub.2017.05.036"},{"key":"e_1_3_4_64_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0092-8674(01)00638-9"},{"key":"e_1_3_4_65_2","doi-asserted-by":"publisher","DOI":"10.1038\/sj.emboj.7600543"},{"key":"e_1_3_4_66_2","doi-asserted-by":"publisher","DOI":"10.1091\/mbc.e12-08-0589"},{"key":"e_1_3_4_67_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-018-04231-7"},{"key":"e_1_3_4_68_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.devcel.2015.11.018"},{"key":"e_1_3_4_69_2","doi-asserted-by":"publisher","DOI":"10.1007\/s00418-012-0988-2"},{"key":"e_1_3_4_70_2","doi-asserted-by":"publisher","DOI":"10.1091\/mbc.e13-03-0156"},{"key":"e_1_3_4_71_2","doi-asserted-by":"publisher","DOI":"10.1002\/cm.20053"},{"key":"e_1_3_4_72_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.cellbi.2005.07.005"},{"key":"e_1_3_4_73_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.274.22.15538"},{"key":"e_1_3_4_74_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pbio.3000317"},{"key":"e_1_3_4_75_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.1094561"},{"key":"e_1_3_4_76_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-024-45878-9"},{"key":"e_1_3_4_77_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41589-019-0405-4"},{"key":"e_1_3_4_78_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41563-020-00825-z"},{"key":"e_1_3_4_79_2","doi-asserted-by":"publisher","DOI":"10.7554\/eLife.60710"},{"key":"e_1_3_4_80_2","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.201806161"},{"key":"e_1_3_4_81_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41580-023-00682-z"},{"key":"e_1_3_4_82_2","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.202203017"},{"key":"e_1_3_4_83_2","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.201711006"},{"key":"e_1_3_4_84_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2011.10.050"},{"key":"e_1_3_4_85_2","doi-asserted-by":"publisher","DOI":"10.1038\/ncb2859"},{"key":"e_1_3_4_86_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pbio.0050221"},{"key":"e_1_3_4_87_2","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.201706052"},{"key":"e_1_3_4_88_2","doi-asserted-by":"publisher","DOI":"10.7554\/eLife.42413"},{"key":"e_1_3_4_89_2","doi-asserted-by":"publisher","DOI":"10.1038\/nrm.2016.172"},{"key":"e_1_3_4_90_2","unstructured":"H. Li Semi-automatic MATLAB code for isolate fiber (Sobel method). GitHub. https:\/\/github.com\/ritterlihao\/semi-automatic-matlab-code-for-isolate-fiber-sobel-. Accessed 27 August 2026."}],"container-title":["Proceedings of the National Academy of Sciences"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/pnas.org\/doi\/pdf\/10.1073\/pnas.2530056123","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,9,8]],"date-time":"2026-09-08T17:47:51Z","timestamp":1788889671000},"score":1,"resource":{"primary":{"URL":"https:\/\/pnas.org\/doi\/10.1073\/pnas.2530056123"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,9,8]]},"references-count":90,"journal-issue":{"issue":"37","published-print":{"date-parts":[[2026,9,15]]}},"alternative-id":["10.1073\/pnas.2530056123"],"URL":"https:\/\/doi.org\/10.1073\/pnas.2530056123","relation":{},"ISSN":["0027-8424","1091-6490"],"issn-type":[{"value":"0027-8424","type":"print"},{"value":"1091-6490","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,9,8]]},"assertion":[{"value":"2025-10-23","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-08-11","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-09-08","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e2530056123"}}