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Natl. Acad. Sci. U.S.A."],"published-print":{"date-parts":[[2026,9,1]]},"abstract":"<jats:p>\n                    Geminiviruses pose a severe threat to grain and vegetable crops worldwide, often resulting in significant economic losses. In cultivated tomato (\n                    <jats:italic toggle=\"yes\">Solanum lycopersicum<\/jats:italic>\n                    ),\n                    <jats:italic toggle=\"yes\">Ty<\/jats:italic>\n                    resistance alleles have been introduced from wild tomato relatives, providing partial to strong resistance to geminivirus infections. The\n                    <jats:italic toggle=\"yes\">Ty-6<\/jats:italic>\n                    resistance locus from\n                    <jats:italic toggle=\"yes\">Solanum chilense<\/jats:italic>\n                    was previously mapped to chromosome 10. It was recently shown to contain a mutant allele of the\n                    <jats:italic toggle=\"yes\">DNA POLYMERASE DELTA 1<\/jats:italic>\n                    (\n                    <jats:italic toggle=\"yes\">POLD1<\/jats:italic>\n                    ) gene that provides resistance to Tomato yellow leaf curl virus (TYLCV) infections. However, the resistance mechanism remained unknown. Here, we report another\n                    <jats:italic toggle=\"yes\">POLD1<\/jats:italic>\n                    allele at the\n                    <jats:italic toggle=\"yes\">Ty-6<\/jats:italic>\n                    locus of\n                    <jats:italic toggle=\"yes\">S. chilense<\/jats:italic>\n                    with an E622D mutation in the catalytic site of the POLD1 protein.\n                    <jats:italic toggle=\"yes\">\n                      POLD1\n                      <jats:sup>E622D<\/jats:sup>\n                    <\/jats:italic>\n                    is maintained as a heterozygous dominant allele in\n                    <jats:italic toggle=\"yes\">S. chilense<\/jats:italic>\n                    and the AVTO2225 breeding line. It provides full resistance to the severe TYLCV Thailand (TYLCTHV) strain. The E622D amino acid change does not alter the predicted structure of POLD1. Replication of the TYLCTHV genome in plants carrying the\n                    <jats:italic toggle=\"yes\">\n                      POLD1\n                      <jats:sup>E622D<\/jats:sup>\n                    <\/jats:italic>\n                    allele is severely compromised by a high frequency of mutations that accumulate in viral DNA, which results in nonfunctional proteins that are essential for continuous viral replication. Ectopically expressing the\n                    <jats:italic toggle=\"yes\">\n                      POLD1\n                      <jats:sup>E622D<\/jats:sup>\n                    <\/jats:italic>\n                    allele cDNA alone causes mutations in TYLCTHV genes in inoculated leaves.\n                    <jats:italic toggle=\"yes\">S. chilense<\/jats:italic>\n                    and AVTO2225 plants carrying the\n                    <jats:italic toggle=\"yes\">\n                      POLD1\n                      <jats:sup>E622D<\/jats:sup>\n                    <\/jats:italic>\n                    allele mount a hypersensitive response after TYLCTHV infection, indicating that the defective virus genome cannot suppress the plant defense. The dominant\n                    <jats:italic toggle=\"yes\">\n                      POLD1\n                      <jats:sup>E622D<\/jats:sup>\n                    <\/jats:italic>\n                    allele is therefore an effective resistance gene that geminiviruses cannot overcome.\n                  <\/jats:p>","DOI":"10.1073\/pnas.2531841123","type":"journal-article","created":{"date-parts":[[2026,8,24]],"date-time":"2026-08-24T19:02:04Z","timestamp":1787598124000},"update-policy":"https:\/\/doi.org\/10.1073\/pnas.cm10313","source":"Crossref","is-referenced-by-count":0,"title":["A dominant mutation in tomato DNA POLYMERASE DELTA 1 causes geminivirus DNA replication catastrophe"],"prefix":"10.1073","volume":"123","author":[{"ORCID":"https:\/\/orcid.org\/0009-0003-9638-9005","authenticated-orcid":false,"given":"Deri","family":"Gustian","sequence":"first","affiliation":[{"name":"Department of Plant Pathology, National Chung Hsing University","place":["Taichung, Taiwan"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5662-3026","authenticated-orcid":false,"given":"Chen-Hsin","family":"Yu","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/047sbcx71","id-type":"ROR","asserted-by":"publisher"}],"name":"Institute of Molecular Biology, Academia Sinica","place":["Taipei, Taiwan"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8312-293X","authenticated-orcid":false,"given":"Fuh-Jyh","family":"Jan","sequence":"additional","affiliation":[{"name":"Department of Plant Pathology, National Chung Hsing University","place":["Taichung, Taiwan"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1872-2998","authenticated-orcid":false,"given":"Wilhelm","family":"Gruissem","sequence":"additional","affiliation":[{"name":"Biotechnology Center, National Chung Hsing University","place":["Taichung, Taiwan"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"341","published-online":{"date-parts":[[2026,8,24]]},"reference":[{"key":"e_1_3_4_1_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-3059.2003.00893.x"},{"key":"e_1_3_4_2_2","doi-asserted-by":"publisher","DOI":"10.1146\/annurev-phyto-072910-095235"},{"key":"e_1_3_4_3_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.virusres.2011.04.018"},{"key":"e_1_3_4_4_2","doi-asserted-by":"publisher","DOI":"10.1099\/vir.0.049627-0"},{"key":"e_1_3_4_5_2","doi-asserted-by":"publisher","DOI":"10.1094\/PDIS.1997.81.12.1358"},{"key":"e_1_3_4_6_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0168-1702(00)00194-5"},{"key":"e_1_3_4_7_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1744-7348.2003.tb00240.x"},{"key":"e_1_3_4_8_2","first-page":"637","article-title":"World management of geminiviruses","volume":"17","author":"Rojas M. 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