{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,6]],"date-time":"2026-10-06T21:50:26Z","timestamp":1791323426217,"version":"4.3.1"},"reference-count":72,"publisher":"National Academy of Sciences","issue":"25","license":[{"start":{"date-parts":[[2026,6,16]],"date-time":"2026-06-16T00:00:00Z","timestamp":1781568000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"funder":[{"DOI":"10.13039\/100014440","name":"Ministerio de Ciencia, Innovaci\u00f3n y Universidades","doi-asserted-by":"publisher","award":["PID2022\u2212136765OB I00"],"award-info":[{"award-number":["PID2022\u2212136765OB I00"]}],"id":[{"id":"10.13039\/100014440","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100014440","name":"Ministerio de Ciencia, Innovaci\u00f3n y Universidades","doi-asserted-by":"publisher","award":["PID2021\u2212123399OB I00"],"award-info":[{"award-number":["PID2021\u2212123399OB I00"]}],"id":[{"id":"10.13039\/100014440","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100004410","name":"European Molecular Biology Organization","doi-asserted-by":"publisher","award":["short term fellowship 11234"],"award-info":[{"award-number":["short term fellowship 11234"]}],"id":[{"id":"10.13039\/100004410","id-type":"DOI","asserted-by":"publisher"}]},{"name":"MOSBRI","award":["MOSBRI 2024 296"],"award-info":[{"award-number":["MOSBRI 2024 296"]}]}],"content-domain":{"domain":["www.pnas.org"],"crossmark-restriction":true},"short-container-title":["Proc. Natl. Acad. Sci. U.S.A."],"published-print":{"date-parts":[[2026,6,23]]},"abstract":"<jats:p>Microtubules are dynamic cytoskeletal polymers whose lattice architecture regulates force generation, nucleotide hydrolysis, and recognition by motor proteins and microtubule-associated proteins (MAPs). Microtubule-stabilizing agents (MSAs), including taxanes and laulimalide\/peloruside-site ligands, suppress depolymerization by binding to defined lattice sites, yet stabilization is not structurally neutral. How ligand chemistry reshapes lattice organization and function remains unresolved. Here, we address three mechanistic questions. First, do distinct ligand classes induce defined lattice states? Using X-ray fiber diffraction, we show that MSAs selectively stabilize two preferred longitudinal conformations, a compact state (~4.06 nm monomer rise) and an expanded state (~4.17 nm), while modulating lateral organization reflected in shifts in mean MT radius. These axial spacings cluster around discrete values across chemotypes, indicating stabilization of preexisting conformational minima rather than continuous distortion. Second, are these states interconvertible upon changes in ligand occupancy? Time-resolved diffraction reveals that longitudinal transitions occur within seconds of ligand addition even at substoichiometric occupancy, whereas, lateral equilibration proceeds slower, consistent with redistribution within heterogeneous protofilament organizations. Third, do such structural states alter nucleotide hydrolysis and motor\/MAP behavior? Expanded lattices are associated with reduced apparent GTP hydrolysis rates under steady-state assembly conditions and altered kinesin motility, whereas compact lattices preferentially promote tau binding and distinct motor interaction profiles. Together, these findings establish longitudinal lattice conformation as a regulatory parameter and position MSAs as chemical tools that bias a dynamic structural landscape with predictable catalytic and transport consequences.<\/jats:p>","DOI":"10.1073\/pnas.2532791123","type":"journal-article","created":{"date-parts":[[2026,6,16]],"date-time":"2026-06-16T16:16:51Z","timestamp":1781626611000},"update-policy":"https:\/\/doi.org\/10.1073\/pnas.cm10313","source":"Crossref","is-referenced-by-count":5,"title":["An atlas of microtubule lattice parameters regulated through ligand binding to the microtubule-stabilizing sites"],"prefix":"10.1073","volume":"123","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-7314-8696","authenticated-orcid":false,"given":"Daniel","family":"Lucena-Agell","sequence":"first","affiliation":[{"id":[{"id":"https:\/\/ror.org\/02gfc7t72","id-type":"ROR","asserted-by":"publisher"}],"name":"Unidad Interdepartamental de Desarrollo de Nuevos F\u00e1rmacos Biol\u00f3gicos, Inmunol\u00f3gicos y Qu\u00edmicos para la Salud Global, Centro de Investigaciones Biol\u00f3gicas Margarita Salas, Consejo Superior de Investigaciones Cient\u00edficas","place":["Madrid, Spain"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0009-2589-1069","authenticated-orcid":false,"given":"\u00d3scar","family":"Fern\u00e1ndez","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/02gfc7t72","id-type":"ROR","asserted-by":"publisher"}],"name":"Unidad Interdepartamental de Desarrollo de Nuevos F\u00e1rmacos Biol\u00f3gicos, Inmunol\u00f3gicos y Qu\u00edmicos para la Salud Global, Centro de Investigaciones Biol\u00f3gicas Margarita Salas, Consejo Superior de Investigaciones Cient\u00edficas","place":["Madrid, Spain"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7953-0949","authenticated-orcid":false,"given":"Rebeca","family":"Par\u00eds-Og\u00e1yar","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/02gfc7t72","id-type":"ROR","asserted-by":"publisher"}],"name":"Unidad Interdepartamental de Desarrollo de Nuevos F\u00e1rmacos Biol\u00f3gicos, Inmunol\u00f3gicos y Qu\u00edmicos para la Salud Global, Centro de Investigaciones Biol\u00f3gicas Margarita Salas, Consejo Superior de Investigaciones Cient\u00edficas","place":["Madrid, Spain"]},{"name":"Instituto Madrile\u00f1o de Estudios Avanzados en Nanociencia","place":["Madrid, Spain"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Juan","family":"Est\u00e9vez-Gallego","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/03eh3y714","id-type":"ROR","asserted-by":"publisher"}],"name":"Laboratory of Biomolecular Research, Department of Biology and Chemistry, Paul Scherrer Institute","place":["Villigen, Switzerland"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0004-1255-6302","authenticated-orcid":false,"given":"Denisa","family":"Ondr\u00fa\u0161kov\u00e1","sequence":"additional","affiliation":[{"name":"Institute of Biotechnology Czech Academy of Sciences, Biotechnology and Biomedicine Centre of the Academy of Sciences and Charles University","place":["Prague West, Czech Republic"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Beatriz","family":"\u00c1lvarez-Bernad","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/02gfc7t72","id-type":"ROR","asserted-by":"publisher"}],"name":"Unidad Interdepartamental de Desarrollo de Nuevos F\u00e1rmacos Biol\u00f3gicos, Inmunol\u00f3gicos y Qu\u00edmicos para la Salud Global, Centro de Investigaciones Biol\u00f3gicas Margarita Salas, Consejo Superior de Investigaciones Cient\u00edficas","place":["Madrid, Spain"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0579-2874","authenticated-orcid":false,"given":"Francesca","family":"Bonato","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/02gfc7t72","id-type":"ROR","asserted-by":"publisher"}],"name":"Unidad Interdepartamental de Desarrollo de Nuevos F\u00e1rmacos Biol\u00f3gicos, Inmunol\u00f3gicos y Qu\u00edmicos para la Salud Global, Centro de Investigaciones Biol\u00f3gicas Margarita Salas, Consejo Superior de Investigaciones Cient\u00edficas","place":["Madrid, Spain"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7293-7239","authenticated-orcid":false,"given":"Jaime","family":"Larraga","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/02gfc7t72","id-type":"ROR","asserted-by":"publisher"}],"name":"Unidad Interdepartamental de Desarrollo de Nuevos F\u00e1rmacos Biol\u00f3gicos, Inmunol\u00f3gicos y Qu\u00edmicos para la Salud Global, Centro de Investigaciones Biol\u00f3gicas Margarita Salas, Consejo Superior de Investigaciones Cient\u00edficas","place":["Madrid, Spain"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0002-7334-8983","authenticated-orcid":false,"given":"Diego Ort\u00edz","family":"de Elguea","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/02gfc7t72","id-type":"ROR","asserted-by":"publisher"}],"name":"Unidad Interdepartamental de Desarrollo de Nuevos F\u00e1rmacos Biol\u00f3gicos, Inmunol\u00f3gicos y Qu\u00edmicos para la Salud Global, Centro de Investigaciones Biol\u00f3gicas Margarita Salas, Consejo Superior de Investigaciones Cient\u00edficas","place":["Madrid, Spain"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0008-0645-6320","authenticated-orcid":false,"given":"Gregorio Javier","family":"Cano","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/02gfc7t72","id-type":"ROR","asserted-by":"publisher"}],"name":"Unidad Interdepartamental de Desarrollo de Nuevos F\u00e1rmacos Biol\u00f3gicos, Inmunol\u00f3gicos y Qu\u00edmicos para la Salud Global, Centro de Investigaciones Biol\u00f3gicas Margarita Salas, Consejo Superior de Investigaciones Cient\u00edficas","place":["Madrid, Spain"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0003-6922-888X","authenticated-orcid":false,"given":"Maarten","family":"Scheers","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/02gfc7t72","id-type":"ROR","asserted-by":"publisher"}],"name":"Unidad Interdepartamental de Desarrollo de Nuevos F\u00e1rmacos Biol\u00f3gicos, Inmunol\u00f3gicos y Qu\u00edmicos para la Salud Global, Centro de Investigaciones Biol\u00f3gicas Margarita Salas, Consejo Superior de Investigaciones Cient\u00edficas","place":["Madrid, Spain"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8939-2255","authenticated-orcid":false,"given":"Hiroshi","family":"Imai","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/035t8zc32","id-type":"ROR","asserted-by":"publisher"}],"name":"Department of Biological Sciences, Graduate School of Science, The University of Osaka","place":["Osaka, Japan"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1379-460X","authenticated-orcid":false,"given":"Toshiki","family":"Yagi","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/0059h1f24","id-type":"ROR","asserted-by":"publisher"}],"name":"Department of Life Sciences, Faculty of Biological Resources, Prefectural University of Hiroshima","place":["Hiroshima, Japan"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4980-8495","authenticated-orcid":false,"given":"Hiroyuki","family":"Iwamoto","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/01xjv7358","id-type":"ROR","asserted-by":"publisher"}],"name":"Research and Utilization Division, Japan Synchrotron Radiation Research Institute, SPring-8","place":["Hyogo, Japan"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2411-5446","authenticated-orcid":false,"given":"Juan","family":"Carlos Mart\u00ednez-Guil","sequence":"additional","affiliation":[{"name":"ALBA Synchrotron, Consorcio para la Construcci\u00f3n, Equipamiento y Explotaci\u00f3n del Laboratorio de Luz de Sincrotr\u00f3n","place":["Cerdanyola del Vall\u00e8s, Spain"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1722-066X","authenticated-orcid":false,"given":"A. Jonathan","family":"Singh","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/0040r6f76","id-type":"ROR","asserted-by":"publisher"}],"name":"Ferrier Research Institute, and Centre for Biodiscovery, Victoria University of Wellington","place":["Lower Hutt, New Zealand"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7658-7421","authenticated-orcid":false,"given":"Robert Alexander","family":"Keyzers","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/0040r6f76","id-type":"ROR","asserted-by":"publisher"}],"name":"School of Chemical and Physical Sciences, and Centre for Biodiscovery, Victoria University of Wellington","place":["Wellington, New Zealand"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7218-4521","authenticated-orcid":false,"given":"Christopher D.","family":"Vanderwal","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/04gyf1771","id-type":"ROR","asserted-by":"publisher"}],"name":"Department of Chemistry, 1102 Natural Sciences II, University of California","place":["Irvine"]},{"id":[{"id":"https:\/\/ror.org\/04gyf1771","id-type":"ROR","asserted-by":"publisher"}],"name":"Department of Pharmaceutical Sciences, University of California","place":["Irvine"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0747-9734","authenticated-orcid":false,"given":"Karl-Heinz","family":"Altmann","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/04n35qp23","id-type":"ROR","asserted-by":"publisher"}],"name":"ETH Zurich, Department of Chemistry and Applied Biosciences, Institute of Pharmaceutical Sciences","place":["Z\u00fcrich, Switzerland"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1623-6750","authenticated-orcid":false,"given":"Johan","family":"Van der Eycken","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/00cv9y106","id-type":"ROR","asserted-by":"publisher"}],"name":"Department of Organic and Macromolecular Chemistry, Faculty of Sciences, Ghent University","place":["Ghent, Belgium"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1473-4086","authenticated-orcid":false,"given":"Valle","family":"Palomo","sequence":"additional","affiliation":[{"name":"Instituto Madrile\u00f1o de Estudios Avanzados en Nanociencia","place":["Madrid, Spain"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wei-Shuo","family":"Fang","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/02drdmm93","id-type":"ROR","asserted-by":"publisher"}],"name":"Institute of Materia Medica, Chinese Academy of Medical Sciences and Peking Union Medical College","place":["Beijing, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3071-4878","authenticated-orcid":false,"given":"Federico","family":"Gago","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/04pmn0e78","id-type":"ROR","asserted-by":"publisher"}],"name":"Department of Biomedical Sciences, University of Alcal\u00e1","place":["Madrid, Spain"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8474-053X","authenticated-orcid":false,"given":"Zden\u011bk","family":"L\u00e1nsk\u00fd","sequence":"additional","affiliation":[{"name":"Institute of Biotechnology Czech Academy of Sciences, Biotechnology and Biomedicine Centre of the Academy of Sciences and Charles University","place":["Prague West, Czech Republic"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2026-2238","authenticated-orcid":false,"given":"Marcus","family":"Braun","sequence":"additional","affiliation":[{"name":"Institute of Biotechnology Czech Academy of Sciences, Biotechnology and Biomedicine Centre of the Academy of Sciences and Charles University","place":["Prague West, Czech Republic"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2215-4639","authenticated-orcid":false,"given":"Mar\u00eda \u00c1.","family":"Oliva","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/02gfc7t72","id-type":"ROR","asserted-by":"publisher"}],"name":"Unidad Interdepartamental de Desarrollo de Nuevos F\u00e1rmacos Biol\u00f3gicos, Inmunol\u00f3gicos y Qu\u00edmicos para la Salud Global, Centro de Investigaciones Biol\u00f3gicas Margarita Salas, Consejo Superior de Investigaciones Cient\u00edficas","place":["Madrid, Spain"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4359-0859","authenticated-orcid":false,"given":"Shinji","family":"Kamimura","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/03qvqb743","id-type":"ROR","asserted-by":"publisher"}],"name":"Department of Biological Sciences, Faculty of Fundamental Science and Engineering, Chuo University","place":["Tokyo, Japan"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2743-3319","authenticated-orcid":false,"given":"J.","family":"Fernando D\u00edaz","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/02gfc7t72","id-type":"ROR","asserted-by":"publisher"}],"name":"Unidad Interdepartamental de Desarrollo de Nuevos F\u00e1rmacos Biol\u00f3gicos, Inmunol\u00f3gicos y Qu\u00edmicos para la Salud Global, Centro de Investigaciones Biol\u00f3gicas Margarita Salas, Consejo Superior de Investigaciones Cient\u00edficas","place":["Madrid, Spain"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"341","published-online":{"date-parts":[[2026,6,16]]},"reference":[{"key":"e_1_3_4_1_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0091-679X(10)97006-4"},{"key":"e_1_3_4_2_2","doi-asserted-by":"publisher","DOI":"10.1126\/sciadv.adf3021"},{"key":"e_1_3_4_3_2","doi-asserted-by":"publisher","DOI":"10.1038\/nature02393"},{"key":"e_1_3_4_4_2","doi-asserted-by":"publisher","DOI":"10.1021\/bi060334m"},{"key":"e_1_3_4_5_2","doi-asserted-by":"publisher","DOI":"10.1007\/978-3-540-87895-7_7"},{"key":"e_1_3_4_6_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.tcb.2018.05.001"},{"key":"e_1_3_4_7_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.2021847118"},{"key":"e_1_3_4_8_2","doi-asserted-by":"publisher","DOI":"10.1002\/anie.202204052"},{"key":"e_1_3_4_9_2","doi-asserted-by":"publisher","DOI":"10.1038\/nature03566"},{"key":"e_1_3_4_10_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1408124111"},{"key":"e_1_3_4_11_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jmb.2016.06.023"},{"key":"e_1_3_4_12_2","doi-asserted-by":"publisher","DOI":"10.1038\/ncomms12103"},{"key":"e_1_3_4_13_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.neuro.2020.10.004"},{"key":"e_1_3_4_14_2","doi-asserted-by":"publisher","DOI":"10.1634\/theoncologist.1-4-240"},{"key":"e_1_3_4_15_2","doi-asserted-by":"publisher","DOI":"10.7554\/eLife.84791"},{"key":"e_1_3_4_16_2","doi-asserted-by":"publisher","DOI":"10.1002\/anie.201307749"},{"key":"e_1_3_4_17_2","doi-asserted-by":"publisher","DOI":"10.1007\/s12640-018-9929-8"},{"key":"e_1_3_4_18_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.advms.2020.04.001"},{"key":"e_1_3_4_19_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.str.2022.11.006"},{"key":"e_1_3_4_20_2","doi-asserted-by":"publisher","DOI":"10.1242\/jcs.062414"},{"key":"e_1_3_4_21_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41565-018-0084-4"},{"key":"e_1_3_4_22_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41589-022-01096-2"},{"key":"e_1_3_4_23_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.neuropharm.2013.08.016"},{"key":"e_1_3_4_24_2","doi-asserted-by":"publisher","DOI":"10.1016\/0022-2836(92)90132-4"},{"key":"e_1_3_4_25_2","doi-asserted-by":"publisher","DOI":"10.7554\/eLife.50155"},{"key":"e_1_3_4_26_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9258(18)31764-2"},{"key":"e_1_3_4_27_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.bpj.2011.11.005"},{"key":"e_1_3_4_28_2","doi-asserted-by":"publisher","DOI":"10.1021\/bi00457a017"},{"key":"e_1_3_4_29_2","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.128.1.117"},{"key":"e_1_3_4_30_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2014.03.053"},{"key":"e_1_3_4_31_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.2114994119"},{"key":"e_1_3_4_32_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.bpj.2009.09.041"},{"key":"e_1_3_4_33_2","unstructured":"F. Bonato Probing the structural signaling of microtubules with engineered chemically controlled filaments. bioRxiv [Preprint] (2026) 10.1101\/2025.10.23.684077 (Accessed 5 January 2026)."},{"key":"e_1_3_4_34_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jmb.2017.01.001"},{"key":"e_1_3_4_35_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.273.50.33803"},{"key":"e_1_3_4_36_2","doi-asserted-by":"publisher","DOI":"10.1007\/s10822-019-00208-w"},{"key":"e_1_3_4_37_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jmb.2025.168996"},{"key":"e_1_3_4_38_2","doi-asserted-by":"publisher","DOI":"10.1038\/nsb0698-451"},{"key":"e_1_3_4_39_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1319854110"},{"key":"e_1_3_4_40_2","doi-asserted-by":"publisher","DOI":"10.1038\/nrm2782"},{"key":"e_1_3_4_41_2","doi-asserted-by":"publisher","DOI":"10.15252\/embj.2022112101"},{"key":"e_1_3_4_42_2","first-page":"225","article-title":"Interaction of Epothilone analogs with the Paclitaxel binding site; relationship between binding affinity, microtubule stabilization, and cytotoxicity","volume":"11","author":"Buey R. M.","year":"2004","unstructured":"R. M. Buey , Interaction of Epothilone analogs with the Paclitaxel binding site; relationship between binding affinity, microtubule stabilization, and cytotoxicity. Chem. Biol. 11, 225\u2013236 (2004).","journal-title":"Chem. Biol."},{"key":"e_1_3_4_43_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.chembiol.2005.09.010"},{"key":"e_1_3_4_44_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.chembiol.2012.05.008"},{"key":"e_1_3_4_45_2","doi-asserted-by":"publisher","DOI":"10.3389\/fneur.2020.590059"},{"key":"e_1_3_4_46_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41556-019-0375-5"},{"key":"e_1_3_4_47_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41556-019-0374-6"},{"key":"e_1_3_4_48_2","doi-asserted-by":"publisher","DOI":"10.1093\/oso\/9780198524946.001.0001"},{"key":"e_1_3_4_49_2","doi-asserted-by":"publisher","DOI":"10.1007\/978-1-59745-336-3_6"},{"key":"e_1_3_4_50_2","doi-asserted-by":"publisher","DOI":"10.1007\/978-1-59745-442-1_2"},{"key":"e_1_3_4_51_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-020-16972-5"},{"key":"e_1_3_4_52_2","doi-asserted-by":"publisher","DOI":"10.1002\/1096-987X(200009)21:12<1049::AID-JCC3>3.0.CO;2-F"},{"key":"e_1_3_4_53_2","doi-asserted-by":"publisher","DOI":"10.1063\/1.3359469"},{"key":"e_1_3_4_54_2","volume-title":"Gaussian 09","author":"Frisch M. J.","year":"2009","unstructured":"M. J. Frisch , Gaussian 09 (Gaussian Inc, Wallingford, CT, 2009)."},{"key":"e_1_3_4_55_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.ejmech.2023.115668"},{"key":"e_1_3_4_56_2","doi-asserted-by":"publisher","DOI":"10.1063\/5.0019056"},{"key":"e_1_3_4_57_2","doi-asserted-by":"publisher","DOI":"10.1039\/c3ob40407b"},{"key":"e_1_3_4_58_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1802637115"},{"key":"e_1_3_4_59_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2015.07.012"},{"key":"e_1_3_4_60_2","doi-asserted-by":"publisher","DOI":"10.1006\/jmbi.2001.5077"},{"key":"e_1_3_4_61_2","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkh368"},{"key":"e_1_3_4_62_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0006-3495(01)76033-X"},{"key":"e_1_3_4_63_2","doi-asserted-by":"publisher","DOI":"10.1021\/acs.jctc.5b00255"},{"key":"e_1_3_4_64_2","doi-asserted-by":"publisher","DOI":"10.1146\/annurev-biophys-052118-115325"},{"key":"e_1_3_4_65_2","doi-asserted-by":"publisher","DOI":"10.1016\/0165-022X(82)90031-8"},{"key":"e_1_3_4_66_2","doi-asserted-by":"publisher","DOI":"10.1038\/nmeth.2019"},{"key":"e_1_3_4_67_2","unstructured":"F. Gago J. F. D\u00edaz M. A. Oliva Probing the structural signaling of microtubules with engineered chemically controlled filaments. ModelArchive. https:\/\/modelarchive.org\/doi\/10.5452\/ma-rkctk. Deposited 29 July 2025."},{"key":"e_1_3_4_68_2","unstructured":"F. Gago J. F. D\u00edaz M. A. Oliva A short fragment of an expanded 13-protofilament microtubule saturated with docetaxel. ModelArchive. https:\/\/modelarchive.org\/doi\/10.5452\/ma-x9q02. Deposited 2 October 2025."},{"key":"e_1_3_4_69_2","unstructured":"F. Gago J. F. D\u00edaz M. A. Oliva A short fragment of a 12-protofilament microtubule saturated with the macrolide natural product epothilone B. ModelArchive. https:\/\/modelarchive.org\/doi\/10.5452\/ma-2embb. Deposited 2 October 2025."},{"key":"e_1_3_4_70_2","unstructured":"F. Gago J. F. D\u00edaz M. A. Oliva A short fragment of a 13-protofilament microtubule saturated with the polyketide natural product (+)-discodermolide. ModelArchive. https:\/\/modelarchive.org\/doi\/10.5452\/ma-ir5i7. Deposited 2 October 2025."},{"key":"e_1_3_4_71_2","unstructured":"F. Gago J. F. D\u00edaz M. A. Oliva A short fragment of a 15-protofilament microtubule with zampanolide covalently bonded to His229 in every beta-tubulin subunit. ModelArchive. https:\/\/modelarchive.org\/doi\/10.5452\/ma-qcuyh. Deposited 2 October 2025."},{"key":"e_1_3_4_72_2","unstructured":"F. Gago J. F. D\u00edaz M. A. Oliva Probing the structural signaling of microtubules with engineered chemically controlled filaments. ModelArchive. https:\/\/modelarchive.org\/doi\/10.5452\/ma-qzvr1. Deposited 2 October 2025."}],"container-title":["Proceedings of the National Academy of Sciences"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/pnas.org\/doi\/pdf\/10.1073\/pnas.2532791123","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,6,16]],"date-time":"2026-06-16T16:17:55Z","timestamp":1781626675000},"score":1,"resource":{"primary":{"URL":"https:\/\/pnas.org\/doi\/10.1073\/pnas.2532791123"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,6,16]]},"references-count":72,"journal-issue":{"issue":"25","published-print":{"date-parts":[[2026,6,23]]}},"alternative-id":["10.1073\/pnas.2532791123"],"URL":"https:\/\/doi.org\/10.1073\/pnas.2532791123","relation":{},"ISSN":["0027-8424","1091-6490"],"issn-type":[{"value":"0027-8424","type":"print"},{"value":"1091-6490","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,6,16]]},"assertion":[{"value":"2025-11-18","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-05-19","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-06-16","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e2532791123"}}