{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,17]],"date-time":"2026-08-17T22:29:41Z","timestamp":1787005781417,"version":"build-2736575974"},"reference-count":43,"publisher":"National Academy of Sciences","issue":"8","license":[{"start":{"date-parts":[[2026,2,20]],"date-time":"2026-02-20T00:00:00Z","timestamp":1771545600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100012166","name":"National Key R&D Program of China","doi-asserted-by":"crossref","award":["2023YFE0125800"],"award-info":[{"award-number":["2023YFE0125800"]}],"id":[{"id":"10.13039\/501100012166","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/501100012166","name":"National Key R&D Program of China","doi-asserted-by":"crossref","award":["2023YFE0113600"],"award-info":[{"award-number":["2023YFE0113600"]}],"id":[{"id":"10.13039\/501100012166","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":["www.pnas.org"],"crossmark-restriction":true},"short-container-title":["Proc. Natl. Acad. Sci. U.S.A."],"published-print":{"date-parts":[[2026,2,24]]},"abstract":"<jats:p>\n                    Using gas chromatography-electroantennographic detection (GC-EAD), gas chromatography-mass spectrometry (GC-MS), and chiral capillary GC with synthetic stereoisomers, followed by laboratory and field validation, we identified the sex pheromone of the copper green chafer (\n                    <jats:italic toggle=\"yes\">Anomala corpulenta<\/jats:italic>\n                    ) as (\n                    <jats:italic toggle=\"yes\">R<\/jats:italic>\n                    )-japonilure, with its antipode, (\n                    <jats:italic toggle=\"yes\">S<\/jats:italic>\n                    )-japonilure, acting as a behavioral antagonist. Single sensillum recordings (SSR) revealed two types of pheromone-responsive sensilla, one narrowly tuned to the sex pheromone, and another that responded to both (\n                    <jats:italic toggle=\"yes\">R<\/jats:italic>\n                    )- and (\n                    <jats:italic toggle=\"yes\">S<\/jats:italic>\n                    )-japonilure, albeit with lower sensitivity to the latter. Functional characterization of\n                    <jats:italic toggle=\"yes\">A. corpulenta<\/jats:italic>\n                    odorant receptors (ORs) by two-electrode voltage clamp (TEVC) led to the identification of two key receptors, AcorOR18, which was selective for (\n                    <jats:italic toggle=\"yes\">R<\/jats:italic>\n                    )-japonilure, and AcorOR29, which was activated by both enantiomers, and 10 ORs sensitive to plant-derived compounds. SSR combined with fluorescence in situ hybridization (FISH) experiments suggested that\n                    <jats:italic toggle=\"yes\">AcorOR18<\/jats:italic>\n                    is expressed in pheromone-specific sensilla, whereas\n                    <jats:italic toggle=\"yes\">AcorOR29<\/jats:italic>\n                    is localized in sensilla responsive to both the pheromone and its antagonist. RNA interference (RNAi) knockdown of either receptor reduced male attraction to (\n                    <jats:italic toggle=\"yes\">R<\/jats:italic>\n                    )-japonilure, while simultaneous knockdown of both genes abolished behavioral responses, confirming that both receptors are required for full pheromone detection. Three orthologous receptors were identified in the Japanese beetle (\n                    <jats:italic toggle=\"yes\">Popillia japonica<\/jats:italic>\n                    ) genome. Two of them, PjapOR42 and PjapOR37, exhibited strict enantioselectivity for (\n                    <jats:italic toggle=\"yes\">R<\/jats:italic>\n                    )-japonilure, whereas PjapOR39 showed a relaxed enantiospecificity, responding to both enantiomers and preferentially to the behavioral antagonist. These results suggest that PjapOR39 may mediate detection of (\n                    <jats:italic toggle=\"yes\">S<\/jats:italic>\n                    )-japonilure and contribute to behavioral antagonism, revealing a shared molecular mechanism of enantiomeric discrimination in scarab beetles.\n                  <\/jats:p>","DOI":"10.1073\/pnas.2532942123","type":"journal-article","created":{"date-parts":[[2026,2,20]],"date-time":"2026-02-20T18:44:21Z","timestamp":1771613061000},"update-policy":"https:\/\/doi.org\/10.1073\/pnas.cm10313","source":"Crossref","is-referenced-by-count":3,"title":["Pheromone receptors for japonilure in\n                    <i>Anomala corpulenta<\/i>\n                    and\n                    <i>Popillia japonica<\/i>"],"prefix":"10.1073","volume":"123","author":[{"ORCID":"https:\/\/orcid.org\/0009-0004-5161-3613","authenticated-orcid":false,"given":"Yinliang","family":"Wang","sequence":"first","affiliation":[{"name":"State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences","place":["Beijing, China"]},{"name":"Agriculture Gene Engineering Research Center of the Ministry of Education, School of Life Sciences Northeast Normal University","place":["Changchun, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kun","family":"Feng","sequence":"additional","affiliation":[{"name":"State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences","place":["Beijing, China"]},{"name":"Agriculture Gene Engineering Research Center of the Ministry of Education, School of Life Sciences Northeast Normal University","place":["Changchun, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0006-6327-7711","authenticated-orcid":false,"given":"Haoqin","family":"Ke","sequence":"additional","affiliation":[{"name":"Agriculture Gene Engineering Research Center of the Ministry of Education, School of Life Sciences Northeast Normal University","place":["Changchun, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Huanhuan","family":"Dong","sequence":"additional","affiliation":[{"name":"State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences","place":["Beijing, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kebin","family":"Li","sequence":"additional","affiliation":[{"name":"State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences","place":["Beijing, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bingzhong","family":"Ren","sequence":"additional","affiliation":[{"name":"Agriculture Gene Engineering Research Center of the Ministry of Education, School of Life Sciences Northeast Normal University","place":["Changchun, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chen","family":"Luo","sequence":"additional","affiliation":[{"name":"College of Plant Protection, Yangzhou University","place":["Yangzhou, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Cheng","family":"Qu","sequence":"additional","affiliation":[{"name":"Institute of Plant Protection, Beijing Academy of Agriculture and Forestry Sciences","place":["Beijing, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ran","family":"Wang","sequence":"additional","affiliation":[{"name":"Institute of Plant Protection, Beijing Academy of Agriculture and Forestry Sciences","place":["Beijing, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jiao","family":"Yin","sequence":"additional","affiliation":[{"name":"State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences","place":["Beijing, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6800-1240","authenticated-orcid":false,"given":"Walter S.","family":"Leal","sequence":"additional","affiliation":[{"name":"Department of Molecular and Cellular Biology, University of California-Davis","place":["Davis"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"341","published-online":{"date-parts":[[2026,2,20]]},"reference":[{"key":"e_1_3_4_1_2","volume-title":"Beetles: Pheromonal Chemists Par Excellence","author":"Tumlinson J. 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