{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,6]],"date-time":"2026-10-06T16:03:02Z","timestamp":1791302582312,"version":"4.1.0"},"reference-count":54,"publisher":"National Academy of Sciences","issue":"11","license":[{"start":{"date-parts":[[2026,3,10]],"date-time":"2026-03-10T00:00:00Z","timestamp":1773100800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"funder":[{"DOI":"10.13039\/100000057","name":"HHS | NIH | National Institute of General Medical Sciences","doi-asserted-by":"publisher","award":["R15GM152959"],"award-info":[{"award-number":["R15GM152959"]}],"id":[{"id":"10.13039\/100000057","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000057","name":"HHS | NIH | National Institute of General Medical Sciences","doi-asserted-by":"publisher","award":["1DP2GM146253"],"award-info":[{"award-number":["1DP2GM146253"]}],"id":[{"id":"10.13039\/100000057","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000060","name":"HHS | NIH | National Institute of Allergy and Infectious Diseases","doi-asserted-by":"publisher","award":["R01AI114261"],"award-info":[{"award-number":["R01AI114261"]}],"id":[{"id":"10.13039\/100000060","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000078","name":"NSF | MPS | Division of Materials Research","doi-asserted-by":"publisher","award":["2205006"],"award-info":[{"award-number":["2205006"]}],"id":[{"id":"10.13039\/100000078","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000879","name":"Alfred P. Sloan Foundation","doi-asserted-by":"publisher","award":["FG-2023-20857"],"award-info":[{"award-number":["FG-2023-20857"]}],"id":[{"id":"10.13039\/100000879","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["www.pnas.org"],"crossmark-restriction":true},"short-container-title":["Proc. Natl. Acad. Sci. U.S.A."],"published-print":{"date-parts":[[2026,3,17]]},"abstract":"<jats:p>\n                    Exopolysaccharides are carbohydrate polymers secreted by bacteria to perform various roles including adhesion to surfaces, protection from environmental stressors, and as key components in the production of biofilms. The human pathogen\n                    <jats:italic toggle=\"yes\">Vibrio cholerae<\/jats:italic>\n                    produces an exopolysaccharide called VPS, which is essential for the formation of its biofilm matrix through crosslinking interactions with a series of secreted accessory proteins. VPS consists of a repeating tetrasaccharide unit consisting of a uniquely modified \u03b1-L-gulose moiety (with N-acetyl, O-acetyl, and amide-linked glycine modifications). Encoded within the cluster containing the biofilm-production genes is a glycoside lyase called RbmB, which cleaves VPS and has been implicated in biofilm dispersal. Here we describe the ~2 \u00c5 X-ray structure of RbmB bound to tetrameric and octameric fragments of VPS representing 10 total monosaccharides of an enzyme\u2013product complex. The structure, along with molecular dynamics simulations and complementary functional analyses of mutant proteins in vitro and in vivo, illustrates how recognition and cleavage of VPS relies on salt-bridging interactions between arginine residues in RbmB and the amide-linked glycine moiety uniquely found on the VPS L-gulose and a ~30\u00b0 bend near the cleavage site that strains the scissile bond. We further present results regarding the localization of RbmB in\n                    <jats:italic toggle=\"yes\">V. cholerae<\/jats:italic>\n                    cells, suggesting that cleavage of VPS by RbmB likely takes place in the periplasm. Sequence conservation suggests that homologous biofilm systems found in many\n                    <jats:italic toggle=\"yes\">Vibrio<\/jats:italic>\n                    species likely utilize a similar VPS cleavage mechanism suggesting that RbmB could be an effective tool for dispersing biofilms across the\n                    <jats:italic toggle=\"yes\">Vibrio<\/jats:italic>\n                    genus and beyond.\n                  <\/jats:p>","DOI":"10.1073\/pnas.2534280123","type":"journal-article","created":{"date-parts":[[2026,3,10]],"date-time":"2026-03-10T17:47:37Z","timestamp":1773164857000},"update-policy":"https:\/\/doi.org\/10.1073\/pnas.cm10313","source":"Crossref","is-referenced-by-count":4,"title":["Crystal structure of\n                    <i>Vibrio cholerae<\/i>\n                    polysaccharide lyase RbmB bound to\n                    <i>Vibrio<\/i>\n                    polysaccharide (VPS) fragments provides insights into substrate recognition and cleavage"],"prefix":"10.1073","volume":"123","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-4740-9474","authenticated-orcid":false,"given":"Ranjuna","family":"Weerasekera","sequence":"first","affiliation":[{"name":"Department of Molecular Biology and Biochemistry, Molecular Biophysics Program, Wesleyan University","place":["Middletown"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0559-6179","authenticated-orcid":false,"given":"Alexis","family":"Moreau","sequence":"additional","affiliation":[{"name":"Department of Molecular, Cellular and Developmental Biology, Yale University","place":["New Haven"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1745-8598","authenticated-orcid":false,"given":"Xin","family":"Huang","sequence":"additional","affiliation":[{"name":"Department of Molecular, Cellular and Developmental Biology, Yale University","place":["New Haven"]},{"name":"Department of Chemistry, Yale University","place":["New Haven"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6855-8954","authenticated-orcid":false,"given":"Anna","family":"Potapova","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Environmental Toxicology, University of California","place":["Santa Cruz"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Carmen","family":"Schwechheimer","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Environmental Toxicology, University of California","place":["Santa Cruz"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0006-9060-9777","authenticated-orcid":false,"given":"Rajan","family":"Kandel","sequence":"additional","affiliation":[{"name":"Complex Carbohydrate Research Center, University of Georgia","place":["Athens"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yun","family":"Huynh","sequence":"additional","affiliation":[{"name":"Department of Molecular Biology and Biochemistry, Molecular Biophysics Program, Wesleyan University","place":["Middletown"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Owen","family":"Cannizzo","sequence":"additional","affiliation":[{"name":"Department of Molecular Biology and Biochemistry, Molecular Biophysics Program, Wesleyan University","place":["Middletown"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ryan","family":"Gordon","sequence":"additional","affiliation":[{"name":"Department of Molecular Biology and Biochemistry, Molecular Biophysics Program, Wesleyan University","place":["Middletown"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Emma","family":"Gerace","sequence":"additional","affiliation":[{"name":"Department of Molecular Biology and Biochemistry, Molecular Biophysics Program, Wesleyan University","place":["Middletown"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alexander J.","family":"Hinbest","sequence":"additional","affiliation":[{"name":"Department of Molecular Biology and Biochemistry, Molecular Biophysics Program, Wesleyan University","place":["Middletown"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yiyan","family":"Yang","sequence":"additional","affiliation":[{"name":"National Library of Medicine, National Institutes of Health","place":["Bethesda"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiaofang","family":"Jiang","sequence":"additional","affiliation":[{"name":"National Library of Medicine, National Institutes of Health","place":["Bethesda"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2400-6293","authenticated-orcid":false,"given":"Robert J.","family":"Woods","sequence":"additional","affiliation":[{"name":"Complex Carbohydrate Research Center, University of Georgia","place":["Athens"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2773-0348","authenticated-orcid":false,"given":"Jing","family":"Yan","sequence":"additional","affiliation":[{"name":"Department of Molecular, Cellular and Developmental Biology, Yale University","place":["New Haven"]},{"name":"Quantitative Biology Institute, Yale University","place":["New Haven"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6384-7167","authenticated-orcid":false,"given":"Fitnat H.","family":"Yildiz","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Environmental Toxicology, University of California","place":["Santa Cruz"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5432-206X","authenticated-orcid":false,"given":"Rich","family":"Olson","sequence":"additional","affiliation":[{"name":"Department of Molecular Biology and Biochemistry, Molecular Biophysics Program, Wesleyan 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Weerasekera Crystal structure of the polysaccharide lyase RbmB from Vibrio cholerae bound to Vibrio Polysaccharide. RCSB Protein Data Bank. https:\/\/www.rcsb.org\/structure\/9OZD. Deposited 5 June 2025.","DOI":"10.2210\/pdb9ozd\/pdb"},{"key":"e_1_3_4_54_2","doi-asserted-by":"crossref","unstructured":"R. Weerasekera Crystal structure of the polysaccharide lyase RbmB from Vibrio cholerae. RCSB Protein Data Bank. https:\/\/www.rcsb.org\/structure\/9OZL. Deposited 5 June 2025.","DOI":"10.2210\/pdb9ozl\/pdb"}],"container-title":["Proceedings of the National Academy of Sciences"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/pnas.org\/doi\/pdf\/10.1073\/pnas.2534280123","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/pnas.org\/doi\/pdf\/10.1073\/pnas.2534280123","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,3,10]],"date-time":"2026-03-10T17:47:44Z","timestamp":1773164864000},"score":1,"resource":{"primary":{"URL":"https:\/\/pnas.org\/doi\/10.1073\/pnas.2534280123"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,3,10]]},"references-count":54,"journal-issue":{"issue":"11","published-print":{"date-parts":[[2026,3,17]]}},"alternative-id":["10.1073\/pnas.2534280123"],"URL":"https:\/\/doi.org\/10.1073\/pnas.2534280123","relation":{},"ISSN":["0027-8424","1091-6490"],"issn-type":[{"value":"0027-8424","type":"print"},{"value":"1091-6490","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,3,10]]},"assertion":[{"value":"2025-12-02","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-02-04","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-03-10","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e2534280123"}}