{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,20]],"date-time":"2026-10-20T19:06:46Z","timestamp":1792523206962,"version":"4.5.0"},"reference-count":62,"publisher":"National Academy of Sciences","issue":"18","license":[{"start":{"date-parts":[[2026,4,30]],"date-time":"2026-04-30T00:00:00Z","timestamp":1777507200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/100000865","name":"Bill and Melinda Gates Foundation","doi-asserted-by":"publisher","award":["OPP1193833"],"award-info":[{"award-number":["OPP1193833"]}],"id":[{"id":"10.13039\/100000865","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000865","name":"Bill and Melinda Gates Foundation","doi-asserted-by":"publisher","award":["INV-007833"],"award-info":[{"award-number":["INV-007833"]}],"id":[{"id":"10.13039\/100000865","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["www.pnas.org"],"crossmark-restriction":true},"short-container-title":["Proc. Natl. Acad. Sci. U.S.A."],"published-print":{"date-parts":[[2026,5,5]]},"abstract":"<jats:p>\n                    Maize lethal necrosis (MLN) is a severe disease caused by the combined infection of maize chlorotic mottle virus (MCMV) and a potyvirus, most often sugarcane mosaic virus (SCMV). This disease seriously threatens food security across sub-Saharan Africa (SSA). We investigated a major-effect quantitative trait locus for resistance on chromosome 6, named the\n                    <jats:italic toggle=\"yes\">maize lethal necrosis susceptibility locus 1<\/jats:italic>\n                    (\n                    <jats:italic toggle=\"yes\">qMLNS1<\/jats:italic>\n                    ), derived from the Thai line KS23-6. Fine mapping and CRISPR-Cas9 editing of the candidate genes within the narrowed 105 kb interval revealed a peroxisomal\n                    <jats:italic toggle=\"yes\">peptidase<\/jats:italic>\n                    as the underlying cause of susceptibility. Confocal microscopy confirmed the localization of the MLNS1 protein within peroxisomes. Targeted knockout of the\n                    <jats:italic toggle=\"yes\">Mlns1<\/jats:italic>\n                    gene in the susceptible elite line CML536 from SSA conferred resistance comparable to KS23-6 in field trials conducted in Naivasha, Kenya. This knockout specifically blocked MCMV accumulation without affecting SCMV. The edited lines showed no yield penalty or agronomic defects under disease-free conditions. Our findings uncover a mechanistic link between a peroxisomal enzyme and viral susceptibility. They also establish a rapid, scalable gene editing strategy for incorporating MLN resistance into elite germplasm, offering a model for combating similar viral diseases in staple crops globally.\n                  <\/jats:p>","DOI":"10.1073\/pnas.2535202123","type":"journal-article","created":{"date-parts":[[2026,4,30]],"date-time":"2026-04-30T18:03:43Z","timestamp":1777572223000},"update-policy":"https:\/\/doi.org\/10.1073\/pnas.cm10313","source":"Crossref","is-referenced-by-count":1,"title":["Targeted knockout of a host peroxisomal peptidase confers field resistance to maize lethal necrosis"],"prefix":"10.1073","volume":"123","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-2312-1353","authenticated-orcid":false,"given":"Mark","family":"Jung","sequence":"first","affiliation":[{"id":[{"id":"https:\/\/ror.org\/02pm1jf23","id-type":"ROR","asserted-by":"publisher"}],"name":"Corteva 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