{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,30]],"date-time":"2026-07-30T14:45:06Z","timestamp":1785422706375,"version":"3.56.0"},"reference-count":62,"publisher":"National Academy of Sciences","issue":"23","license":[{"start":{"date-parts":[[2026,6,2]],"date-time":"2026-06-02T00:00:00Z","timestamp":1780358400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001809","name":"MOST | National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["32141001"],"award-info":[{"award-number":["32141001"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["www.pnas.org"],"crossmark-restriction":true},"short-container-title":["Proc. Natl. Acad. Sci. U.S.A."],"published-print":{"date-parts":[[2026,6,9]]},"abstract":"<jats:p>\n                    Dominant carbapenem-resistant\n                    <jats:italic toggle=\"yes\">\n                      <jats:italic toggle=\"yes\">Klebsiella pneumoniae<\/jats:italic>\n                    <\/jats:italic>\n                    (CRKP) clones have markedly intensified the burden of invasive infections while severely restricting treatment options. Although genomic determinants of these high-risk lineages are well characterized, adaptive regulatory mechanisms beyond fixed DNA variation remain poorly understood. Here we systematically map the adenosine-to-inosine (A-to-I) mRNA editing landscape in CRKP and reveal that clinically dominant lineages possess a constrained and clone-specific landscape of RNA editing. We identify PncR (a previously uncharacterized AraC\/XylS family transcription factor) and the response regulator DcuR as prominent A-to-I editing targets enriched in high-risk clones. Editing of these regulators reshapes redox and metabolic programs, thereby increasing tolerance to oxidative stress associated with innate immune attack. These results support a model in which stress-responsive RNA editing promotes stress adaptation specifically in high-risk CRKP clones. Furthermore, we demonstrate that the tRNA deaminase TadA is the sole A-to-I editing enzyme in\n                    <jats:italic toggle=\"yes\">\n                      <jats:italic toggle=\"yes\">K. pneumoniae<\/jats:italic>\n                    <\/jats:italic>\n                    , and that its abundance determines both the extent and diversity of mRNA editing. These findings establish TadA-driven A-to-I editing as a selectable, posttranscriptional regulatory layer that reshapes bacterial metabolism and promotes the success of high-risk CRKP lineages, highlighting this axis as a potential target for therapeutic intervention.\n                  <\/jats:p>","DOI":"10.1073\/pnas.2536397123","type":"journal-article","created":{"date-parts":[[2026,6,2]],"date-time":"2026-06-02T16:20:03Z","timestamp":1780417203000},"update-policy":"https:\/\/doi.org\/10.1073\/pnas.cm10313","source":"Crossref","is-referenced-by-count":0,"title":["TadA-mediated A-to-I mRNA editing rewires redox metabolism to promote dominance of epidemic\n                    <i>\n                      <i>Klebsiella pneumoniae<\/i>\n                    <\/i>\n                    clones"],"prefix":"10.1073","volume":"123","author":[{"given":"Xingyu","family":"Wu","sequence":"first","affiliation":[{"id":[{"id":"https:\/\/ror.org\/035adwg89","id-type":"ROR","asserted-by":"publisher"}],"name":"Department of Clinical Laboratory, Peking University People\u2019s Hospital","place":["Beijing, China"]},{"name":"Beijing Key Laboratory of Innovative & Transformable Warning and Intervention Technologies for Drug-Resistant Pathogens","place":["Beijing, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9010-4994","authenticated-orcid":false,"given":"Longyang","family":"Jin","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/035adwg89","id-type":"ROR","asserted-by":"publisher"}],"name":"Department of Clinical Laboratory, Peking University People\u2019s Hospital","place":["Beijing, China"]},{"name":"Beijing Key Laboratory of Innovative & Transformable Warning and Intervention Technologies for 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Intervention Technologies for Drug-Resistant Pathogens","place":["Beijing, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kaiwen","family":"Song","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/035adwg89","id-type":"ROR","asserted-by":"publisher"}],"name":"Department of Clinical Laboratory, Peking University People\u2019s Hospital","place":["Beijing, China"]},{"name":"Beijing Key Laboratory of Innovative & Transformable Warning and Intervention Technologies for Drug-Resistant Pathogens","place":["Beijing, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Long","family":"Chen","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/035adwg89","id-type":"ROR","asserted-by":"publisher"}],"name":"Department of Clinical Laboratory, Peking University People\u2019s Hospital","place":["Beijing, China"]},{"name":"Beijing Key Laboratory of Innovative & Transformable Warning and Intervention Technologies for Drug-Resistant Pathogens","place":["Beijing, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Guankun","family":"Yin","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/035adwg89","id-type":"ROR","asserted-by":"publisher"}],"name":"Department of Clinical Laboratory, Peking University People\u2019s Hospital","place":["Beijing, China"]},{"name":"Beijing Key Laboratory of Innovative & Transformable Warning and Intervention Technologies for Drug-Resistant Pathogens","place":["Beijing, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fengning","family":"Chen","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/035adwg89","id-type":"ROR","asserted-by":"publisher"}],"name":"Department of Clinical Laboratory, Peking University People\u2019s Hospital","place":["Beijing, China"]},{"name":"Beijing Key Laboratory of Innovative & Transformable Warning and Intervention Technologies for Drug-Resistant Pathogens","place":["Beijing, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiaoyun","family":"Liu","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Infectious Disease Center, National Health Commission Key Laboratory of Medical Immunology, School of Basic Medical Sciences, Peking University Health Science Center","place":["Beijing, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9220-0357","authenticated-orcid":false,"given":"Hui","family":"Wang","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/035adwg89","id-type":"ROR","asserted-by":"publisher"}],"name":"Department of Clinical Laboratory, Peking University People\u2019s Hospital","place":["Beijing, China"]},{"name":"Beijing Key Laboratory of Innovative & Transformable Warning and Intervention Technologies for Drug-Resistant Pathogens","place":["Beijing, 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Wu Genomic sequencing and RNA-seq data for Klebsiella pneumoniae clinical isolates. NCBI BioProject. https:\/\/www.ncbi.nlm.nih.gov\/bioproject\/PRJNA1467212. 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