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Natl. Acad. Sci. U.S.A."],"published-print":{"date-parts":[[2026,9,15]]},"abstract":"<jats:p>\n                    Of the ~1,100 mitochondrial proteins, only a handful like PINK1 and ATFS-1 are known to stabilize and relocalize upon collapse of the proton motive force (PMF) to execute signaling roles. To systematically identify genes that increase exclusively at the protein level upon PMF collapse, we performed a joint proteomic and RNA-seq screen. The screen revealed 10 candidates (six mitochondrial), including two genes in vitamin B\n                    <jats:sub>12<\/jats:sub>\n                    metabolism - the B\n                    <jats:sub>12<\/jats:sub>\n                    chaperone MMADHC and cytosolic B\n                    <jats:sub>12<\/jats:sub>\n                    -dependent 5-methyltetrahydrofolate-homocysteine methyltransferase (MTR). MMADHC is short-lived across cell types and we show that its levels increase with PMF collapse. MMADHC stabilization precedes PINK1 activation in a time course of increasing mtDNA depletion, suggesting greater sensitivity to PMF collapse. MMADHC accumulates in mitochondria with LONP1 inhibition but in the cytosol upon PMF collapse, likely due to mitochondrial import failure. Cytosol-stabilized MMADHC increases MTR levels and activity. Altogether, the mitochondrial PMF regulates the cytosolic B\n                    <jats:sub>12<\/jats:sub>\n                    -dependent MTR, integral to one-carbon metabolism, by controlling the stability and compartmentalization of the B\n                    <jats:sub>12<\/jats:sub>\n                    chaperone MMADHC.\n                  <\/jats:p>","DOI":"10.1073\/pnas.2537797123","type":"journal-article","created":{"date-parts":[[2026,9,8]],"date-time":"2026-09-08T17:47:37Z","timestamp":1788889657000},"update-policy":"https:\/\/doi.org\/10.1073\/pnas.cm10313","source":"Crossref","is-referenced-by-count":0,"title":["Mitochondrial depolarization stabilizes the vitamin B\n                    <sub>12<\/sub>\n                    chaperone MMADHC in the cytosol to increase MTR activity"],"prefix":"10.1073","volume":"123","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-8839-1285","authenticated-orcid":false,"given":"Sneha P.","family":"Rath","sequence":"first","affiliation":[{"id":[{"id":"https:\/\/ror.org\/002pd6e78","id-type":"ROR","asserted-by":"publisher"}],"name":"Department of Molecular Biology, Mass General Hospital","place":["Boston"]},{"name":"Department of Systems Biology and Medicine, Harvard Medical School","place":["Boston"]},{"id":[{"id":"https:\/\/ror.org\/042nb2s44","id-type":"ROR","asserted-by":"publisher"}],"name":"Broad Institute of Massachusetts Institute of Technology and Harvard","place":["Cambridge"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhu","family":"Li","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/00jmfr291","id-type":"ROR","asserted-by":"publisher"}],"name":"Department of Biological Chemistry, University of Michigan","place":["Ann Arbor"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Arkajit","family":"Guha","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/00jmfr291","id-type":"ROR","asserted-by":"publisher"}],"name":"Department of Biological Chemistry, University of Michigan","place":["Ann Arbor"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fangcong","family":"Dong","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/002pd6e78","id-type":"ROR","asserted-by":"publisher"}],"name":"Department of Molecular Biology, Mass General Hospital","place":["Boston"]},{"name":"Department of Systems Biology and Medicine, Harvard Medical School","place":["Boston"]},{"id":[{"id":"https:\/\/ror.org\/042nb2s44","id-type":"ROR","asserted-by":"publisher"}],"name":"Broad Institute of Massachusetts Institute of Technology and Harvard","place":["Cambridge"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8332-3275","authenticated-orcid":false,"given":"Ruma","family":"Banerjee","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/00jmfr291","id-type":"ROR","asserted-by":"publisher"}],"name":"Department of Biological Chemistry, University of Michigan","place":["Ann Arbor"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9924-642X","authenticated-orcid":false,"given":"Vamsi K.","family":"Mootha","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/002pd6e78","id-type":"ROR","asserted-by":"publisher"}],"name":"Department of Molecular Biology, Mass General Hospital","place":["Boston"]},{"name":"Department of Systems Biology and Medicine, Harvard Medical School","place":["Boston"]},{"id":[{"id":"https:\/\/ror.org\/042nb2s44","id-type":"ROR","asserted-by":"publisher"}],"name":"Broad Institute of Massachusetts Institute of Technology and Harvard","place":["Cambridge"]},{"name":"HHMI","place":["Boston"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"341","published-online":{"date-parts":[[2026,9,8]]},"reference":[{"key":"e_1_3_4_1_2","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkaa1011"},{"key":"e_1_3_4_2_2","doi-asserted-by":"publisher","DOI":"10.1038\/nrm2959"},{"key":"e_1_3_4_3_2","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.201008084"},{"key":"e_1_3_4_4_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41556-021-00840-5"},{"key":"e_1_3_4_5_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pbio.1000298"},{"key":"e_1_3_4_6_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.1223560"},{"key":"e_1_3_4_7_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41586-020-2076-4"},{"key":"e_1_3_4_8_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41586-020-2078-2"},{"key":"e_1_3_4_9_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.molcel.2023.05.031"},{"key":"e_1_3_4_10_2","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.biochem.72.121801.161828"},{"key":"e_1_3_4_11_2","doi-asserted-by":"publisher","DOI":"10.1096\/fasebj.4.5.2407589"},{"key":"e_1_3_4_12_2","volume-title":"Methylmalonyl-CoA Mutase","author":"Banerjee R.","year":"1999","unstructured":"R. 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