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We show that the vas deferens actively modulates luminal geometry in response to these flow dynamics: ROCK activity in smooth muscle is required for global contraction and cAMP-associated signaling modulates this contractile response. By contrast, extracellular signal-regulated kinase activity in circumferential smooth muscle is dispensable for the ductal contraction but essential for active, flow-dependent remodeling of the distal lumen, forming the core of the mechano-signaling module that couples sperm flow to valve opening. 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