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Natl. Acad. Sci. U.S.A."],"published-print":{"date-parts":[[2026,8,25]]},"abstract":"<jats:p>\n                    <jats:italic toggle=\"yes\">Rhodnius prolixus<\/jats:italic>\n                    , an insect vector of Chagas disease, ingests blood meals several-fold its own body weight. This nutritional overload triggers specific adaptive responses that prevent or remediate damage from multiple stressors, including oxidative, osmotic, and microbial challenges. Transcriptomic analysis of first-instar nymph digestive apparatus revealed five members of the small Heat Shock Protein (sHSP) family that were highly expressed after blood meals but were downregulated upon\n                    <jats:italic toggle=\"yes\">Trypanosoma cruzi<\/jats:italic>\n                    infection. sHSPs are proteins involved in cellular homeostasis and stress responses. Simultaneous knockdown of all five sHSPs profoundly disrupted insect physiology, causing decreased actin filament network formation, interrupted peristalsis, blocked endoplasmic reticulum (ER) expansion, and reduced ER\u2013mitochondria association, while increasing reactive oxygen species generation and inducing premature epithelial cell mitosis. This apparent loss of digestive apparatus homeostasis was recapitulated by trypanosome infection and, conversely, sHSPs silencing in infected insects increased parasite numbers. Taken together, our data reveal a paramount role for sHSPs in midgut cell homeostasis following blood meals and support the hypothesis that sHSPs expression down regulation represents an adaptive manipulation of the insect host by the parasite.\n                  <\/jats:p>","DOI":"10.1073\/pnas.2600647123","type":"journal-article","created":{"date-parts":[[2026,8,17]],"date-time":"2026-08-17T19:03:46Z","timestamp":1786993426000},"update-policy":"https:\/\/doi.org\/10.1073\/pnas.cm10313","source":"Crossref","is-referenced-by-count":0,"title":["The role of small Heat Shock Proteins in\n                    <i>Trypanosoma cruzi<\/i>\n                    infection and intestinal homeostasis in a Chagas disease vector"],"prefix":"10.1073","volume":"123","author":[{"given":"Tainan C.","family":"Guedes-Silva","sequence":"first","affiliation":[{"id":[{"id":"https:\/\/ror.org\/03490as77","id-type":"ROR","asserted-by":"publisher"}],"name":"Laborat\u00f3rio de Bioqu\u00edmica de Artr\u00f3podes Hemat\u00f3fagos, Instituto de Bioqu\u00edmica M\u00e9dica Leopoldo de Meis, Universidade Federal do Rio de Janeiro","place":["Rio de Janeiro, Brazil"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ana B.","family":"Walter-Nuno","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/03490as77","id-type":"ROR","asserted-by":"publisher"}],"name":"Laborat\u00f3rio de Bioqu\u00edmica de Artr\u00f3podes Hemat\u00f3fagos, Instituto de Bioqu\u00edmica M\u00e9dica Leopoldo de Meis, Universidade Federal do Rio de Janeiro","place":["Rio de Janeiro, Brazil"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"J\u00e9ssica C. 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