{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,29]],"date-time":"2026-09-29T17:02:36Z","timestamp":1790701356763,"version":"4.1.0"},"reference-count":47,"publisher":"National Academy of Sciences","issue":"40","license":[{"start":{"date-parts":[[2026,9,29]],"date-time":"2026-09-29T00:00:00Z","timestamp":1790640000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"content-domain":{"domain":["www.pnas.org"],"crossmark-restriction":true},"short-container-title":["Proc. Natl. Acad. Sci. U.S.A."],"published-print":{"date-parts":[[2026,10,6]]},"abstract":"<jats:p>\n                    Vascular endothelial cells express abundant sialylated glycans, but their function remains unclear. To determine the role of endothelial overall sialylation, which is controlled by\n                    <jats:italic toggle=\"yes\">Slc35a1<\/jats:italic>\n                    , in adult mice. We generated mice with inducible deletion of\n                    <jats:italic toggle=\"yes\">Slc35a1<\/jats:italic>\n                    in endothelial cells (iEHC\n                    <jats:italic toggle=\"yes\">Slc35a1<\/jats:italic>\n                    <jats:sup>\u2013\/\u2013<\/jats:sup>\n                    ) using tamoxifen-inducible\n                    <jats:italic toggle=\"yes\">\n                      Cdh5Cre\n                      <jats:sup>ERT2<\/jats:sup>\n                    <\/jats:italic>\n                    . Induced deletion of\n                    <jats:italic toggle=\"yes\">Slc35a1<\/jats:italic>\n                    in adulthood caused progressive weight loss, anemia, and spontaneous intestinal bleeding. Adult intestinal villus capillaries exhibited disorganized networks, loss of vascular integrity, and bleeding. Multiplexed Error-Robust Fluorescence in Situ Hybridization spatial transcriptomic analysis revealed downregulation of endothelial genes essential for angiogenesis and endothelial specialization, including\n                    <jats:italic toggle=\"yes\">Vegfr2<\/jats:italic>\n                    ,\n                    <jats:italic toggle=\"yes\">Cdh5<\/jats:italic>\n                    , and\n                    <jats:italic toggle=\"yes\">Plvap<\/jats:italic>\n                    . Immunostaining confirmed loss of VEGFR2, disorganized VE-cadherin junctions, and impaired PLVAP-dependent capillary fenestration. Endothelial\n                    <jats:italic toggle=\"yes\">Slc35a1<\/jats:italic>\n                    deletion also reduced endothelial von Willebrand factor expression and plasma multimerization, indicating impaired hemostatic function. Our results reveal new roles for\n                    <jats:italic toggle=\"yes\">Slc35a1<\/jats:italic>\n                    -dependent endothelial sialylation in maintaining the fate and integrity of the intestinal microvascular network in adult mice.\n                  <\/jats:p>","DOI":"10.1073\/pnas.2601083123","type":"journal-article","created":{"date-parts":[[2026,9,29]],"date-time":"2026-09-29T16:18:26Z","timestamp":1790698706000},"update-policy":"https:\/\/doi.org\/10.1073\/pnas.cm10313","source":"Crossref","is-referenced-by-count":0,"title":["Slc35a1-mediated endothelial sialylation controls the fate and integrity of the intestinal microvascular network in adult mice"],"prefix":"10.1073","volume":"123","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-9386-3678","authenticated-orcid":false,"given":"Yizhi","family":"Jiang","sequence":"first","affiliation":[{"id":[{"id":"https:\/\/ror.org\/035z6xf33","id-type":"ROR","asserted-by":"publisher"}],"name":"Cardiovascular Biology Research Program, Oklahoma Medical Research Foundation","place":["Oklahoma City"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shipra","family":"Rathore","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/035z6xf33","id-type":"ROR","asserted-by":"publisher"}],"name":"Cardiovascular Biology Research Program, Oklahoma Medical Research Foundation","place":["Oklahoma City"]},{"id":[{"id":"https:\/\/ror.org\/0457zbj98","id-type":"ROR","asserted-by":"publisher"}],"name":"Department of Biochemistry and Physiology, University of Oklahoma Health Sciences Center","place":["Oklahoma City"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xin","family":"Geng","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/035z6xf33","id-type":"ROR","asserted-by":"publisher"}],"name":"Cardiovascular Biology Research Program, Oklahoma Medical Research Foundation","place":["Oklahoma City"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0007-8923-8144","authenticated-orcid":false,"given":"Nicole","family":"Kirby","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/035z6xf33","id-type":"ROR","asserted-by":"publisher"}],"name":"Cardiovascular Biology Research Program, Oklahoma Medical Research Foundation","place":["Oklahoma City"]},{"id":[{"id":"https:\/\/ror.org\/0457zbj98","id-type":"ROR","asserted-by":"publisher"}],"name":"Department of Biochemistry and Physiology, University of Oklahoma Health Sciences Center","place":["Oklahoma City"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0003-3461-4772","authenticated-orcid":false,"given":"Chihiro","family":"Ito","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/035z6xf33","id-type":"ROR","asserted-by":"publisher"}],"name":"Cardiovascular Biology Research Program, Oklahoma Medical Research Foundation","place":["Oklahoma City"]},{"id":[{"id":"https:\/\/ror.org\/04chrp450","id-type":"ROR","asserted-by":"publisher"}],"name":"Department of Biochemistry II, Nagoya University School of Medicine","place":["Showa-ku, Nagoya, Japan"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"J. Michael","family":"McDaniel","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/035z6xf33","id-type":"ROR","asserted-by":"publisher"}],"name":"Cardiovascular Biology Research Program, Oklahoma Medical Research Foundation","place":["Oklahoma City"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Meixiang","family":"Zhou","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/035z6xf33","id-type":"ROR","asserted-by":"publisher"}],"name":"Cardiovascular Biology Research Program, Oklahoma Medical Research Foundation","place":["Oklahoma City"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Samuel","family":"McGee","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/035z6xf33","id-type":"ROR","asserted-by":"publisher"}],"name":"Cardiovascular Biology Research Program, Oklahoma Medical Research Foundation","place":["Oklahoma City"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4465-3340","authenticated-orcid":false,"given":"R. 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