{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,20]],"date-time":"2026-08-20T18:26:08Z","timestamp":1787250368479,"version":"build-2736575974"},"reference-count":49,"publisher":"National Academy of Sciences","issue":"34","license":[{"start":{"date-parts":[[2026,8,20]],"date-time":"2026-08-20T00:00:00Z","timestamp":1787184000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"funder":[{"DOI":"10.13039\/100000152","name":"NSF | BIO | Division of Molecular and Cellular Biosciences","doi-asserted-by":"publisher","award":["MCB-1947720"],"award-info":[{"award-number":["MCB-1947720"]}],"id":[{"id":"10.13039\/100000152","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["www.pnas.org"],"crossmark-restriction":true},"short-container-title":["Proc. Natl. Acad. Sci. U.S.A."],"published-print":{"date-parts":[[2026,8,25]]},"abstract":"\n De novo proteins that share no ancestry with natural sequences can serve as additions to the evolved proteomes of living cells. Upon expression in cells, these novel proteins can provide biological functions that alter cell viability and growth. To isolate such proteins, we searched a combinatorial library of novel sequences by selecting for sequences that sustain the growth of\n Escherichia coli<\/jats:italic>\n under conditions where the recipient cell would otherwise be inviable. This led to the identification of\n Resc4<\/jats:italic>\n (\n Rescuer 4<\/jats:italic>\n ), a de novo protein that sustains growth on minimal medium of an\n E. coli<\/jats:italic>\n strain harboring a lethal deletion of\n metC<\/jats:italic>\n , which encodes cystathionine\n \n \n \u03b2<\/mml:mi>\n <\/mml:math>\n <\/jats:inline-formula>\n -lyase, a conditionally essential enzyme in the biosynthesis of methionine. Surprisingly, despite its ability to rescue the deletion of a biosynthetic enzyme,\n Resc4<\/jats:italic>\n is insoluble. Nonetheless,\n Resc4<\/jats:italic>\n sustains the growth of\n \n \n \u0394<\/mml:mi>\n <\/mml:math>\n <\/jats:inline-formula>\n metC<\/jats:italic>\n cells by upregulating expression of\n metB<\/jats:italic>\n , which encodes a different enzyme, cystathionine\n \n \n \u03b3<\/mml:mi>\n <\/mml:math>\n <\/jats:inline-formula>\n -synthase, which has a moonlighting activity that compensates for the deleted activity encoded by\n metC<\/jats:italic>\n . Proteomic analysis revealed that\n Resc4<\/jats:italic>\n sequesters MetJ, the repressor of the methionine biosynthesis operon. Sequestration of MetJ leads to overproduction of cystathionine\n \n \n \u03b3<\/mml:mi>\n <\/mml:math>\n <\/jats:inline-formula>\n -synthase, thereby allowing it to rescue the deletion of\n metC<\/jats:italic>\n . These results, taken together with previous findings on other de novo proteins, demonstrate that novel proteins added to a cell\u2019s proteome can perform life-sustaining functions, and may shed light on de novo gene birth\u2014both in synthetic biology and in natural evolution.\n <\/jats:p>","DOI":"10.1073\/pnas.2601204123","type":"journal-article","created":{"date-parts":[[2026,8,20]],"date-time":"2026-08-20T17:54:21Z","timestamp":1787248461000},"update-policy":"https:\/\/doi.org\/10.1073\/pnas.cm10313","source":"Crossref","is-referenced-by-count":0,"title":["An insoluble de novo protein enables survival of\n Escherichia coli<\/i>\n by sequestering a gene repressor"],"prefix":"10.1073","volume":"123","author":[{"ORCID":"https:\/\/orcid.org\/0009-0009-5786-7477","authenticated-orcid":false,"given":"Guanyu","family":"Liao","sequence":"first","affiliation":[{"id":[{"id":"https:\/\/ror.org\/00hx57361","id-type":"ROR","asserted-by":"publisher"}],"name":"Department of Chemistry, Princeton University","place":["Princeton"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sha","family":"Tao","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/00hx57361","id-type":"ROR","asserted-by":"publisher"}],"name":"Department of Chemistry, Princeton University","place":["Princeton"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jessica L.","family":"Dessau","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/00hx57361","id-type":"ROR","asserted-by":"publisher"}],"name":"Department of Chemistry, Princeton University","place":["Princeton"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0002-4473-5212","authenticated-orcid":false,"given":"Yejin","family":"Bann","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/00hx57361","id-type":"ROR","asserted-by":"publisher"}],"name":"Department of Chemistry, Princeton University","place":["Princeton"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5538-9813","authenticated-orcid":false,"given":"Michael H.","family":"Hecht","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/00hx57361","id-type":"ROR","asserted-by":"publisher"}],"name":"Department of Chemistry, Princeton University","place":["Princeton"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"341","published-online":{"date-parts":[[2026,8,20]]},"reference":[{"key":"e_1_3_4_1_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.sbi.2017.11.009"},{"key":"e_1_3_4_2_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0015364"},{"key":"e_1_3_4_3_2","doi-asserted-by":"publisher","DOI":"10.1002\/pro.2871"},{"key":"e_1_3_4_4_2","doi-asserted-by":"publisher","DOI":"10.1002\/pro.2611"},{"key":"e_1_3_4_5_2","doi-asserted-by":"publisher","DOI":"10.1038\/nchembio.2550"},{"key":"e_1_3_4_6_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.2218281120"},{"key":"e_1_3_4_7_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1600566113"},{"key":"e_1_3_4_8_2","doi-asserted-by":"publisher","DOI":"10.1021\/acssynbio.6b00336"},{"key":"e_1_3_4_9_2","doi-asserted-by":"crossref","unstructured":"I. 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